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Shinichi Ikemoto Seiji Wada Masato Kamizuru Nobuyuki Hayahara Taketoshi Kishimoto Masanobu Maekawa 《Cancer immunology, immunotherapy : CII》1992,34(5):289-293
Summary The authors examined interleukin-2 (IL-2) production and interferon (IFN) production of peripheral blood mononuclear cells in 28 patients with renal cell carcinoma and 17 control subjects. The peripheral blood was obtained prior to the initiation of therapeutic procedures. The patients were divided into two groups according to tumor size, 5 cm and >5 cm. The production of IL-2 and IFN was measured by immunoradiometric assay. As a result, in the patients with tumors >5 cm, IL-2 and IFN production was impaired. However, in the patients with tumors 5 cm, IFN production was enhanced, though IL-2 production was not significantly different from that of the control subjects. There was no significant correlation between IL-2 production and IFN production. 相似文献
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Albert Faradji Alain Bohbot Marion Schmitt-Goguel Norbert Roeslin Serge Dumont Marie-Louise Wiesel Christian Lallot Michel Eber Jacques Bartholeyns Philippe Poindron Georges Morand Jean-Paul Witz Francis Oberling 《Cancer immunology, immunotherapy : CII》1991,33(5):319-326
Summary The purpose of this phase I study was to evaluate the toxicity and biological activity of autologous blood-derived macrophages activated ex-vivo with recombinant human interferon (rhuIFN) [monokine-activated killer (MAK) cells] and administered intravenously to 11 lung cancer patients once a week for 6 consecutive weeks. Peripheral blood monocytes were collected by leukapheresis and then purified by counterflow elutriation. The MAK cells were generated by culturing the purified monocytes in Teflon bags for 7 days and adding rhuIFN to the cultured cells for the last 18 h. These MAK cells expressed differentiation-associated surface antigen MAX1, and were cytotoxic in vitro against tumour cell line U937. The MAK cells were infused at dose levels from 1 × 107 to 5 × 108 on an intrapatient dose-escalating schedule. No severe adverse side-effects occurred. Toxicity was mild to moderate [primarly fever (75%) and chills (32%)], non-dose-dependent, and non-cumulative. No consistent change in haemostatic function, or liver or renal function was observed. Dose-limiting toxicity was not reached at 5 × 108 cells (optimal dose reproduced for each patient). The maximum tolerated dose was not determined. The immunomodulatory activity of i.v. infused MAK cells was demonstrated both in vivo by significant increases in granulocyte count and neopterin level in the patients' peripheral blood postinfusion and in vitro by secretory products (IL-1. TNF, neopterin, and thromboplastin-like substance) in the culture supernatants. The in vivo traffic patterns of autologous MAK cells labelled ex-vivo with111In oxine were studied in 7 patients. Gamma imaging showed an immediate but transient lung uptake (<24 h), and a progressive uptake of radioactivity in the liver and spleen was seen from 6 h to 72 h post-infusion. Our results indicate that the preparation of high numbers of autologous, blood-derived MAK cells is a feasible procedure, and their transfusion is safe for patients. This immunotherapeutic approach seems to be encouraging from the point of view of establishing an adjuvant therapeutic modality in cancer patients with minimal residual disease.This work was supported in part by a grant 6911 from the Association pour la Recherche contre le Cancer (ARC), grants from the Ligue Nationale contre le cancer and the Ligues Regionales (Bas-Rhin, Haut-Rhin) contre le cancer, and contract 891013 from the Institut National pour la Santé et la Recherche Médicale (INSERM), France 相似文献
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子宫颈糜烂病毒病因的探讨 总被引:17,自引:0,他引:17
491份宫颈拭子病毒分离结果表明:糜烂宫颈单纯疱疹病毒(HSV)分离阳性率(30.8%)是正常宫颈(2.6%)的11.8倍,用人干扰素治疗一个疗程后,病毒分离率下降至疗前的1/4.36例糜烂宫颈活体组织DNA分子杂交表明,乳头瘤病毒16型(HPV-16)阳性者占52.8和,HPV-18占17.9%,HPV-6B占28.1%,HPV-11占7.7%,251例宫颈糜烂患者经人(?)D型基因工程干扰素双盲对比治疗后,总有效率达93.8%,显效率达60%,分析临床疗效与HSV分离率的变化表明,临床有效病例中有35%(49/140)在治疗后病毒阴转,有57%在疗前疗后均未分离出HSV,有5%在疗前疗后保持阳性不变,有2.9%疗前阴性,疗后阳性,上述结果表明,HSV和HPV与慢性宫颈炎有一定关系。 相似文献
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利用DNA重组技术,去掉人γ干扰素(IFNγ)基因3′端含编码多肽羧基(C)端11个氨基酸的核苷酸序列,与编码P,G,I,L的DNA序列相连接,构成IFNγ突变体(rIFN-γ132-PGIL)基因,将后者插入pBV220P_RP_L串连启动子下游,转化大肠杆菌DH5α,在CIts857基因的调节下,通过升温诱导,获得了高效表达,表达量约占菌体可溶性蛋白的30%以上,抗病毒活性平均可达4.9×10~-8U/L,比母体菌株高10倍。SDS—PAGE结果表明,rIFN-γ132—PGIL分子量为17kd。 相似文献
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A. C. Passaquin G. Coupin W. A. Schreier P. Poindron R. A. Cole J. de Vellis 《Neurochemical research》1989,14(10):987-993
We investigated the effect of rat interferon-/ (IFN) on the expression of glycerol phosphate dehydrogenase (E.C.1.1.1.8; GPDH), in both C6 cells and pure cultures of oligodendrocytes. IFNs are naturally produced inhibitors of cell growth that can also affect differentiated cell functions. GPDH is a biochemical marker for oligodendrocytes and is known to be developmentally regulated and steroid inducible. GPDH activity is induced by hydrocortisone (HC) 3.5 fold in C6 cells and 5 fold in oligodendrocytes compared to untreated cultures. A pretreatment of these cells with 75 U/ml of rat IFN-/ resulted in an inhibition of the HC induction of GPDH enzymatic activity by 50% and 40% in C6 cells and oligodendrocytes respectively. We also found that IFN impaired the accumulation of GPDH mRNA in both cell types. These results demonstrate that IFNs are capable of modifying the cellular response to hormones in cells of neuroepithelial origin, and suggest the possibility that IFNs may be able to influence the development and function of the brain.Special issue dedicated to Dr. Paola S. Timiras 相似文献
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本文报道小剂量干扰素与胸腺肽联合对慢性乙肝20例抗病毒效应的追踪观察结果,并与同期同批同剂量单纯干扰素治疗13例慢性乙肝作对照,追踪时间均为治后半年—2年,从HBeAg、HBcAg、DNAP、HBV DNA阴转率来看,治疗组分别为58.8%、60%、60%及66.6%、对照组分别为50%、50%、100%及50%。再从HBV四项复制指标改变来看,则治疗组4例全阴转,7例仅一项阳性,总有效率达61.1%(11/18),而对照组仅为20%(2/10),P<0.01,认为干扰素与胸腺肽联合治疗优于对照组,并扼要讨论增强干扰素抗病毒效应的各种措施,认为干扰素与胸腺肽联合,不论从前已报道近期疗效和本文远期追踪来看均较安全而有效,二者合用起到增强抗病毒效应的作用,值得进一步探索。 相似文献
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Induction of Nitric Oxide Synthase in Rat C6 Glioma Cells 总被引:9,自引:1,他引:8
Douglas L. Feinstein Elena Galea Steven Roberts Henrik Berquist Hong Wang Donald J. Reis 《Journal of neurochemistry》1994,62(1):315-321
Abstract: We have examined the induction of nitric oxide syhthase (NOS) activity in the rat astrocyte-derived C6 glioma cell line. In contrast to the previous results with primary astrocyte cultures, incubation of C6 cells with bacterial endotoxin lipopolysaccharide (LPS; 1 μg/ml for 24 h) did not stimulate NO2 production. However, addition of either tumor necrosis factor-a (TNF-α) or interferon-γ (IFN-γ), cytokines that by themselves had no effect on NOS activity, imparted LPS responsiveness onto these cells in a dose-dependent manner (EC50 values of 39 ng/ml of TNF-α and 9.4 U/ml of IFN-γ), and the effect of TNF-α could be further potentiated (twofold) by the presence of interleukin-1β. The simultaneous presence of TNF-α and IFN-γ yielded a greater response than either cytokine alone; however, the respective EC50 values were not affected. A cytoplasmic extract from induced C6 cells catalyzed the Ca2+ -independent conversion of l -arginine to l - citrulline, with an apparent K m of 51.2 n M , and this activity could be blocked by l -arginine analogues in the potency order amino > methyl > nitroarginine. Immunoblot analysis revealed an apparent molecular mass of 125 kDa for the NOS protein induced in C6 cells. These results indicate that the combination of LPS plus cytokines can induce NOS activity in C6 glioma cells with properties similar to those of the enzyme expressed in primary astrocyte cultures. 相似文献