全文获取类型
收费全文 | 3131篇 |
免费 | 274篇 |
国内免费 | 96篇 |
出版年
2024年 | 7篇 |
2023年 | 69篇 |
2022年 | 38篇 |
2021年 | 107篇 |
2020年 | 120篇 |
2019年 | 142篇 |
2018年 | 106篇 |
2017年 | 92篇 |
2016年 | 127篇 |
2015年 | 117篇 |
2014年 | 141篇 |
2013年 | 292篇 |
2012年 | 125篇 |
2011年 | 127篇 |
2010年 | 83篇 |
2009年 | 121篇 |
2008年 | 116篇 |
2007年 | 128篇 |
2006年 | 103篇 |
2005年 | 95篇 |
2004年 | 81篇 |
2003年 | 90篇 |
2002年 | 94篇 |
2001年 | 79篇 |
2000年 | 74篇 |
1999年 | 64篇 |
1998年 | 60篇 |
1997年 | 59篇 |
1996年 | 47篇 |
1995年 | 40篇 |
1994年 | 37篇 |
1993年 | 35篇 |
1992年 | 47篇 |
1991年 | 39篇 |
1990年 | 43篇 |
1989年 | 28篇 |
1988年 | 32篇 |
1987年 | 24篇 |
1986年 | 26篇 |
1985年 | 38篇 |
1984年 | 32篇 |
1983年 | 30篇 |
1982年 | 34篇 |
1981年 | 24篇 |
1980年 | 23篇 |
1979年 | 22篇 |
1978年 | 6篇 |
1977年 | 9篇 |
1975年 | 7篇 |
1974年 | 7篇 |
排序方式: 共有3501条查询结果,搜索用时 46 毫秒
1.
H. DELATTE S. A. JULIANO D. FONTENILLE S. QUILICI 《Medical and veterinary entomology》2013,27(4):387-397
Two invasive, container‐breeding mosquito species, Aedes aegypti (Stegomyia aegypti) and Aedes albopictus (Stegomyia albopicta) (Diptera: Culicidae), have different distribution patterns on Reunion Island. Aedes albopictus occurs in all areas and Ae. aegypti colonizes only some restricted areas already occupied by Ae. albopictus. This study investigates the abiotic and biotic ecological mechanisms that determine the distribution of Aedes species on Reunion Island. Life history traits (duration of immature stages, survivorship, fecundity, estimated finite rate of increase) in Ae. aegypti and Ae. albopictus were compared at different temperatures. These fitness measures were characterized in both species in response to competitive interactions among larvae. Aedes aegypti was drastically affected by temperature, performing well only at around 25 °C, at which it achieved its highest survivorship and greatest estimated rate of increase. The narrow distribution of this species in the field on Reunion Island may thus relate to its poor ability to cope with unfavourable temperatures. Aedes aegypti was also more negatively affected by high population densities and to some extent by interactions with Ae. albopictus, particularly in the context of limited food supplies. Aedes albopictus exhibited better population performance across a range of environmental conditions. Its ecological plasticity and its superior competitive ability relative to its congener may further enhance its invasion success on Reunion Island. 相似文献
2.
Barbara A. Booth Jouni Uitto 《Biochimica et Biophysica Acta (BBA)/General Subjects》1981,675(1):117-122
Human skin fibroblasts were cultured under conditions optimized for collagen synthesis, and the effects of ascorbic acid on procollagen production, proline hydroxylation and the activity of prolyl hydroxylase were examined in cultures. The results indicated that addition of ascorbic acid to confluent monolayer cultures of adult human skin fibroblasts markedly increased tha amount of [3H]hydroxyproline syntehsized. Ascorbic acid, however, did not increase the synthesis of 3H-labeled collagenous polypeptides assayed independently of hydroxylation of proline residues, nor did it affect the amount of prolyl hydroxylase detectable by an in vitro enzyme assay. Also long-term cultures of the cells or initiation of fibroblast cultures in the presence of ascorbic acid did not lead to an apparent selection of a cell population which might be abnormally responsive to ascorbic acid. Thus, ascorbic acid appears to have one primary action on the synthesis of procollagen by cultured human skin fibroblasts: it is necessary for synthesis of hydroxyproline, and consequently for proper triple helix formation and selection of procollagen. 相似文献
3.
4.
Immediate fragmentation of parental DNA by near-ultraviolet irradiation at 313 nm was measured in cultured skin fibroblasts from normal individuals, patients with Xeroderma pigmentosum of complementation group A (XPA) and Xeroderma pigmentosum variants (XPV) by the alkaline elution procedure. For a dose of 2.25 KJm?2 given at Oo fragmentation was comparable in all cell strains. However, fragmentation was strongly increased relative to Oo in XPV but not in normal fibroblasts and the XPA strains when irradiation was carried out at 37o. From our results it appears that a step in the repair of DNA is abnormal in XPV. 相似文献
5.
David O. Simelane 《Biocontrol Science and Technology》2006,16(9):971-979
Compatibility between the root-feeding agent Longitarsus bethae Savini & Escalona and the varieties of the target weed Lantana camara L. could influence the chances of successful establishment and effectiveness of this agent as a biocontrol agent of lantana in South Africa. Laboratory experiments were conducted to determine the influence of major South African lantana varieties on feeding, colonization, oviposition preference and larval survival of L. bethae. Feeding preference and the number of adults per plant varied among the varieties. The most damaged and colonized varieties were 150 Orange, 009 Light Pink and 113 Dark Pink. Variety 018 Dark Pink was the least damaged, while 021 Total Pink and 029 White Pink were the least colonized. Females laid the highest number of eggs on variety 150 Orange. The lowest number of eggs was recorded on Australian Richmond Pink and the closely related species Lippia wilmsii. Larval survival differed significantly among the varieties. The highest survival was recorded on 150 Orange. Duration of development also varied significantly among the varieties, with the slowest rate of development recorded on variety 029 Light Pink and the quickest on variety 015 White Yellow. Although variations in female body size were less significant among varieties, males emerging from variety 150 Orange were slightly smaller than those emerged from other varieties. There was a significant tendency for females to select varieties that enhanced the survival of their offspring, which met the predictions of the preference-performance hypothesis. The data suggest that the chances of establishment and successful control of the weed are expected to be much greater on those varieties that were preferred for oviposition and provided better larval performance. 相似文献
6.
Francesca Zito Demian Koop Maria Byrne Valeria Matranga 《Development, growth & differentiation》2015,57(7):507-514
Carbonic anhydrases (CAs) are a family of widely distributed metalloenzymes, involved in diverse physiological processes. These enzymes catalyse the reversible conversion of carbon dioxide to protons and bicarbonate. At least 19 genes encoding for CAs have been identified in the sea urchin genome, with one of these localized to the skeletogenic mesoderm (primary mesenchyme cells, PMCs). We investigated the effects of a specific inhibitor of CA, acetazolamide (AZ), on development of two sea urchin species with contrasting investment in skeleton production, Paracentrotus lividus and Heliocidaris tuberculata, to determine the role of CA on PMC differentiation, skeletogenesis and on non‐skeletogenic mesodermal (NSM) cells. Embryos were cultured in the presence of AZ from the blastula stage prior to skeleton formation and development to the larval stage was monitored. At the dose of 8 mmol/L AZ, 98% and 90% of P. lividus and H. tuberculata embryos lacked skeleton, respectively. Nevertheless, an almost normal PMC differentiation was indicated by the expression of msp130, a PMC‐specific marker. Strikingly, the AZ‐treated embryos also lacked the echinochrome pigment produced by the pigment cells, a subpopulation of NSM cells with immune activities within the larva. Conversely, all ectoderm and endoderm derivatives and other subpopulations of mesoderm developed normally. The inhibitory effects of AZ were completely reversed after removal of the inhibitor from the medium. Our data, together with new information concerning the involvement of CA on skeleton formation, provide evidence for the first time of a possible role of the CAs in larval immune pigment cells. 相似文献
7.
皮肤作为人体最大器官覆盖于全身,能阻挡有害物质的侵入,保护人体内环境稳态,参与人体代谢过程。皮肤损伤、炎症和纤维化等,都会导致皮肤屏障功能的减退,影响正常的生命活动。溶血磷脂酸(lysophosphatidic acid,LPA)是十分活跃的磷脂信号分子,参与多种生理和病理生理过程。LPA是维持体内平衡所必需的生物活性脂质介质,在皮肤中通过不同的信号通路发挥多功能磷脂信使作用。本文综述了皮肤中溶血磷脂酸受体(lysophosphatidic acid receptor,LPA1-6)及其细胞信号通路的作用及机制,综述了LPA在皮肤创面愈合、皮肤瘢痕、皮肤黑色素瘤、硬皮病、皮肤瘙痒、过敏性皮炎、皮肤屏障、皮肤疼痛,皮肤毛发生长中的作用及分子机制,有助于了解LPA在皮肤中的生理和病理生理作用。深入研究LPA的作用机制将有助于挖掘其在皮肤治疗中的作用,开发以LPA为靶点的药物。 相似文献
8.
We studied the hibernation behavior of the water frog Rana lessonae and its hybridogenetic associate R. esculenta in their natural habitat during three successive winters. Animals caught in pitfall traps at a fenced pond were individually
marked with PIT tags and some (n=36) were additionally equipped with radio transmitters. Of the animals caught, 85% left the fenced pond for hibernation.
More R. esculenta remained inside the fenced area compared to R. lessonae. R. lessonae emigrated earlier in autumn and came back later in spring than R. esculenta, but the distance to their hibernation sites did not differ. Both species left the fenced pond earlier in the year when ambient
temperatures were lower. All radio-tracked animals hibernated in woodland, 3–7 cm below the surface in soil, under moss, fallen
leaves or small branches. Soil temperatures at the actual hibernation sites were significantly higher than at randomly chosen
control sites. A surprising finding was that most frogs changed their hibernation sites during winter, and often more than
once. Movements were more frequent in the warmer first half of the winter than in the cooler second half, but some animals
were active even on days with mean temperatures below 1°C. These results show that both species do not spend the whole winter
torpid in one particular hibernation site but move around, especially at higher temperatures. Most of the animals lost weight
during the winter, and the weight loss was greater in females than in males and higher in warm than in cold winters. To what
extent weight loss and survival is influenced by the chosen hibernation sites and the amount of movement during winter, and
whether this contributes to the differences in species and sex ratios found in mixed populations, needs more investigation.
Received: 4 August 1999 / Accepted: 15 November 1999 相似文献
9.
Climatic forcing and larval dispersal capabilities shape the replenishment of fishes and their habitat‐forming biota on a tropical coral reef 下载免费PDF全文
Shaun K. Wilson Martial Depcyznski Rebecca Fisher Thomas H. Holmes Mae M. Noble Ben T. Radford Michael Rule George Shedrawi Paul Tinkler Christopher J. Fulton 《Ecology and evolution》2018,8(3):1918-1928
Fluctuations in marine populations often relate to the supply of recruits by oceanic currents. Variation in these currents is typically driven by large‐scale changes in climate, in particular ENSO (El Nino Southern Oscillation). The dependence on large‐scale climatic changes may, however, be modified by early life history traits of marine taxa. Based on eight years of annual surveys, along 150 km of coastline, we examined how ENSO influenced abundance of juvenile fish, coral spat, and canopy‐forming macroalgae. We then investigated what traits make populations of some fish families more reliant on the ENSO relationship than others. Abundance of juvenile fish and coral recruits was generally positively correlated with the Southern Oscillation Index (SOI), higher densities recorded during La Niña years, when the ENSO‐influenced Leeuwin Current is stronger and sea surface temperature higher. The relationship is typically positive and stronger among fish families with shorter pelagic larval durations and stronger swimming abilities. The relationship is also stronger at sites on the coral back reef, although the strongest of all relationships were among the lethrinids (r = .9), siganids (r = .9), and mullids (r = .8), which recruit to macroalgal meadows in the lagoon. ENSO effects on habitat seem to moderate SOI–juvenile abundance relationship. Macroalgal canopies are higher during La Niña years, providing more favorable habitat for juvenile fish and strengthening the SOI effect on juvenile abundance. Conversely, loss of coral following a La Niña‐related heat wave may have compromised postsettlement survival of coral dependent species, weakening the influence of SOI on their abundance. This assessment of ENSO effects on tropical fish and habitat‐forming biota and how it is mediated by functional ecology improves our ability to predict and manage changes in the replenishment of marine populations. 相似文献
10.
Abstract This paper deals in detail with the morphology of the larva of Neopsylla specialis specialis Jordan, 1932. It may be distinguished from other larvae of 5 species or subspecies of Neopsylla by two fine setae lying on outside of each posterior long seta on the ventral plates of the first to third thoracic segments, ratio of the length and width of the egg burster, number and shape of mandibular teeth, number and length of the setae in the anterior and posterior row on dorsal side of head, and number of the setae of anal comb and the strut setae. The sense organs on the 10th tergite are discussed. 相似文献