Expression and characterization of an <Emphasis Type="Italic">α</Emphasis>-glucosidase from <Emphasis Type="Italic">Thermoanaerobacter ethanolicus</Emphasis> JW200 with potential for industrial application

In this study, a new α-glucosidase gene from Thermoanaerobacter ethanolicus JW200 was cloned and expressed in Escherichia coli by a novel heat-shock vector pHsh. The recombinant α-glucosidase exhibited its maximum hydrolytic activity at 70°C and pH 5.0∼5.5. With p-nitrophenyl-α-D-glucoside as a substrate and under the optimal condition (70°C, pH 5.5), K _m and V _max of the enzyme was 1.72 mM and 39 U/mg, respectively. The purified α-glucosidase could hydrolyze oligosaccharides with both α-1,4 and α-1,6 linkages. The enzyme also had strong transglycosylation activity when maltose was used as sugar donor. The transglucosylation products towards maltose are isomaltose, maltotriose, panose, isomaltotriose and tetrasaccharides. The enzyme could convert 400 g/L maltose to oligosaccharides with a conversion rate of 52%, and 83% of the oligosaccharides formed were prebiotic isomaltooligosaccharides (containing isomaltose, panose and isomaltotriose).     

湖南蕨类植物区系新资料

报道了湖南蕨类植物区系新记录17种（隶属7科,11属）,它们是桫椤（Alsophila spinulosa）、长尾铁线蕨（Adiantunm diaphanum）半月形铁线蕨（A. philippense）、新月蕨（Pronephrium gymnopteridifrons）、密毛紫柄蕨（Pseudophegopteris hirtirachis）、禾秆紫柄蕨（P. microstegia）、金佛山溪边蕨（Stegnogramma jinfoshanensis）、假大羽铁角蕨（Asplenium pseudolaserpitiifolium）、都匀铁角蕨（A. toramanum）、长镰羽耳蕨（Polystichum falcatilobum）、瓦鳞耳蕨（P. fimbriatum）、棕鳞耳蕨（P. polyblepharum）、倒鳞耳蕨（P. retroso-paleaceum）、膜边肋毛蕨（Ctenitis clarkei）、三叉蕨（Tecaria subtriphylla）、指叶假瘤蕨（Phymatopteris dactylina）、掌叶线蕨（Colysis digitata）。     

Quantum chemical study on influence of substituents and solvents in reaction complexing ethylene with nickel dithiolene

The influences induced by various terminal substituents and solvents on the reaction mechanism and chemical dynamics of complexing ethylene with Ni dithiolene are theoretically studied by using B3LYP method and Onsager model. It is shown that the reaction should be a two-step process, and the first step is the rate-determining step. We find that the rate constant of the rate-determining step becomes small when the electron-donating ability of the substituents is increased, while it becomes large when the electron-withdrawing ability of the substituents is increased. Subsequently, we consider the solvent effects on the reaction adding ethylene to the simplified hydrogen-substituted nickel dithiolene. It is found that the solvents will make slight changes to the geometries of the reactants, transition states, intermediates and products. However, the corresponding molecular dipole moments become large with the increase of the solvent polarity, which is beneficial to accelerate the reaction. Moreover, we show that, as the solvent polarity becomes large, the activation energies of the reaction decrease exponentially, while the reaction rate constants increase exponentially. These results demonstrate that in polar solvents, the reaction complexing ethylene with Ni dithiolene may become easier and faster to occur, and the product rate is improved. We believe that this research can be seen as a reference for complex and solvent selection in olefin separation process.     

Generation and Characterization of a Nanobody Against SARS-CoV

Virologica Sinica - The sudden emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) has caused global panic in 2003, and the risk of SARS-CoV outbreak still exists. However, no...     

中国双盖蕨属一新记录种——Diplazium×kidoi Sa.Kurata

首次报道了中国双盖蕨属一新记录种,中日双盖蕨（Diplazium × kidoi Sa. Kurata）,该种是耳羽短肠蕨（D. wichurae（Mett.）Diels）和薄叶双盖蕨（D. pinfaense Ching）的杂交种。本研究详细描述了中日双盖蕨的形态特征,并比较了其与两个亲本的差别。D × kidoi的发现对研究异域杂交物种形成和认识蕨类植物物种形成中广泛存在的杂交现象具有一定的意义。     

14种剑蕨属植物的孢子形态特征及其系统分类学意义

利用扫描电子显微镜对水龙骨科(Polypodiaceae)剑蕨属(Loxogramme)14种植物的孢子形态和表面纹饰进行了观察。结果显示:剑蕨属的孢子具有单裂缝和三裂缝两种类型,个别物种具有中间过渡类型。剑蕨属的孢子纹饰为蠕虫状或浅波纹状。经比较发现,具三裂缝孢子的物种通常植株较小,而具单裂缝的种类则通常植株较大。从进化的角度来看,三裂缝孢子为原始类型,单裂缝孢子为进化类型,因而从孢子形态特征的角度支持剑蕨属为水龙骨科基部类群。     

Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41

A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.     

自发性高血压大鼠多组织炎症状态

高血压是一种慢性血管性疾病,易累及肾、肝、心、脑等组织,引起脑卒中和心、肾损害等并发症.本研究对高血压时肾、肝、心、脑等组织的炎症状态进行了观察.实验采用自发性高血压大鼠(spontaneously hypertensive rat,SHR)和正常血压的Wistar-Kyoto(WKY)大鼠,用RT-PCR和Western blot法观察肾、肝、心、脑等组织炎症相关因子IL-1p、TNFα、ICAM-1、iNOS、C/EBPδ和PPARγ的基因表达;紫外分光光度法观察蛋白质羰基化水平和FRAP法检测组织总抗氧化能力.结果显示(1)SHR组织炎症相关因子表达较对照WKY增强,除IL-1βmRNA在肝和脑的增加不明显外,其余均有显著性差异(P＜0.05);(2)SHR和WKY大鼠肾、心、脑蛋白质羰基化水平(nmol/mg蛋白)分别为8.93±1.08和2.27±0.43、2.23±0.23和0.17±0.02、13.42±1.10和5.72±1.01,SHR明显增加(P＜0.05);而肝脏蛋白质羰基化水平无明显变化;(3)SHR肾、肝、心、脑总抗氧化能力水平显著低于WKY大鼠(P＜0.05).以上结果表明,SHR多个组织(肾、肝、心和脑)均存在炎症因子被诱导和氧化应激反应等明显的炎症状态,提示炎症可能在高血压及其并发症的病理改变中起重要作用.     

人重组GM－CSF／HAS－D3嵌合蛋白（GM－HSA）在大肠杆菌中的表达及其产物稳定性的研究

将人粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）和人血清白蛋白第三功能区（ＨＡＳ－Ｄ３）的基因串联后,在Ｅ．ｃｏｌｉ中获高效表达,表达量占菌体蛋白的３２．６％．利用ＴＦ－１体外细胞活性测定表明,ＧＭ－ＨＳＡ的活性单位为１．０４×１０~６Ｕ／ｍｇ,虽然其比活性低于ＧＭ－ＣＳＦ,但比后者具有更高的体外热稳定性和储藏稳定性．