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排序方式: 共有225条查询结果,搜索用时 203 毫秒
1.
Effect of propionate and pyruvate on citrulline synthesis and ATP content in rat liver mitochondria.
L Cathelineau F P Petit F X Coudé P P Kamoun 《Biochemical and biophysical research communications》1979,90(1):327-332
Propionate inhibits citrullinogenesis when succinate (plus rotenone) or glutamate are the oxidizable substrates used. Propionate decreases the intramitochondrial concentration of carbamylphosphate by decreasing the ATP content. When the energy supply for citrullinogenesis is provided by an influx of exogenous ATP, propionate is no longer an inhibitor. Pyruvate inhibits citrullinogenesis with glutamate but not with succinate (plus rotenone) as oxidizable substrates. Propionate and pyruvate deplete mitochondrial ATP but probably by different mechanisms. 相似文献
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Tolga O. Bozkurt Khaoula Belhaj Yasin F. Dagdas Angela Chaparro‐Garcia Chih‐Hang Wu Liliana M. Cano Sophien Kamoun 《Traffic (Copenhagen, Denmark)》2015,16(2):204-226
A number of plant pathogenic and symbiotic microbes produce specialized cellular structures that invade host cells where they remain enveloped by host‐derived membranes. The mechanisms underlying the biogenesis and functions of host–microbe interfaces are poorly understood. Here, we show that plant late endocytic trafficking is diverted toward the extrahaustorial membrane (EHM); a host–pathogen interface that develops in plant cells invaded by Irish potato famine pathogen Phytophthora infestans. A late endosome and tonoplast marker protein Rab7 GTPase RabG3c, but not a tonoplast‐localized sucrose transporter, is recruited to the EHM, suggesting specific rerouting of vacuole‐targeted late endosomes to a host–pathogen interface. We revealed the dynamic nature of this process by showing that, upon activation, a cell surface immune receptor traffics toward the haustorial interface. Our work provides insight into the biogenesis of the EHM and reveals dynamic processes that recruit membrane compartments and immune receptors to this host–pathogen interface. 相似文献
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Hiroki Takagi Akira Abe Kentaro Yoshida Shunichi Kosugi Satoshi Natsume Chikako Mitsuoka Aiko Uemura Hiroe Utsushi Muluneh Tamiru Shohei Takuno Hideki Innan Liliana M. Cano Sophien Kamoun Ryohei Terauchi 《The Plant journal : for cell and molecular biology》2013,74(1):174-183
The majority of agronomically important crop traits are quantitative, meaning that they are controlled by multiple genes each with a small effect (quantitative trait loci, QTLs). Mapping and isolation of QTLs is important for efficient crop breeding by marker‐assisted selection (MAS) and for a better understanding of the molecular mechanisms underlying the traits. However, since it requires the development and selection of DNA markers for linkage analysis, QTL analysis has been time‐consuming and labor‐intensive. Here we report the rapid identification of plant QTLs by whole‐genome resequencing of DNAs from two populations each composed of 20–50 individuals showing extreme opposite trait values for a given phenotype in a segregating progeny. We propose to name this approach QTL‐seq as applied to plant species. We applied QTL‐seq to rice recombinant inbred lines and F2 populations and successfully identified QTLs for important agronomic traits, such as partial resistance to the fungal rice blast disease and seedling vigor. Simulation study showed that QTL‐seq is able to detect QTLs over wide ranges of experimental variables, and the method can be generally applied in population genomics studies to rapidly identify genomic regions that underwent artificial or natural selective sweeps. 相似文献
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F. Ben Rebah F. Frikha W. Kamoun L. Belbahri Y. Gargouri N. Miled 《Letters in applied microbiology》2008,47(6):549-554
Aims: The objective of this study was to demonstrate that fish‐processing by‐products could be used as sole raw material to sustain the growth of Staphylococcus xylosus for lipase production. Methods and Results: Bacterial growth was tested on supernatants generated by boiling (100°C for 20 min) of tuna, sardine, cuttlefish and shrimp by‐products from fish processing industries. Among all samples tested, only supernatants generated from shrimp and cuttlefish by‐products sustained the growth of S. xylosus. Shrimp‐based medium gave the highest growth (A600 = 22) after 22 h of culture and exhibited the maximum lipase activity (28 U ml?1). This effect may be explained by better availability of nutrients, especially, in shrimp by‐products. Standard medium (SM) amendments to sardine and tuna by‐product‐based media stimulated the growth of S. xylosus and the highest A600 values were obtained with 75% SM. Lipase activity, however, remained below 4 U ml?1 for both sardine and tuna by‐product‐based media. Conclusions: Fish by‐products could be used for the production of highly valuable enzymes. Significance and Impact of the Study: The use of fish by‐products in producing S. xylosus‐growth media can reduce environmental problems associated with waste disposal and, simultaneously, lower the cost of biomass and enzyme production. 相似文献
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Kui Lin Erik Limpens Zhonghua Zhang Sergey Ivanov Diane G. O. Saunders Desheng Mu Erli Pang Huifen Cao Hwangho Cha Tao Lin Qian Zhou Yi Shang Ying Li Trupti Sharma Robin van Velzen Norbert de Ruijter Duur K. Aanen Joe Win Sophien Kamoun Ton Bisseling René Geurts Sanwen Huang 《PLoS genetics》2014,10(1)
Nuclei of arbuscular endomycorrhizal fungi have been described as highly diverse due to their asexual nature and absence of a single cell stage with only one nucleus. This has raised fundamental questions concerning speciation, selection and transmission of the genetic make-up to next generations. Although this concept has become textbook knowledge, it is only based on studying a few loci, including 45S rDNA. To provide a more comprehensive insight into the genetic makeup of arbuscular endomycorrhizal fungi, we applied de novo genome sequencing of individual nuclei of Rhizophagus irregularis. This revealed a surprisingly low level of polymorphism between nuclei. In contrast, within a nucleus, the 45S rDNA repeat unit turned out to be highly diverged. This finding demystifies a long-lasting hypothesis on the complex genetic makeup of arbuscular endomycorrhizal fungi. Subsequent genome assembly resulted in the first draft reference genome sequence of an arbuscular endomycorrhizal fungus. Its length is 141 Mbps, representing over 27,000 protein-coding gene models. We used the genomic sequence to reinvestigate the phylogenetic relationships of Rhizophagus irregularis with other fungal phyla. This unambiguously demonstrated that Glomeromycota are more closely related to Mucoromycotina than to its postulated sister Dikarya. 相似文献
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Thorsten Langner Adeline Harant Luis B. Gomez-Luciano Ram K. Shrestha Angus Malmgren Sergio M. Latorre Hernn A. Burbano Joe Win Sophien Kamoun 《PLoS genetics》2021,17(2)
Supernumerary mini-chromosomes–a unique type of genomic structural variation–have been implicated in the emergence of virulence traits in plant pathogenic fungi. However, the mechanisms that facilitate the emergence and maintenance of mini-chromosomes across fungi remain poorly understood. In the blast fungus Magnaporthe oryzae (Syn. Pyricularia oryzae), mini-chromosomes have been first described in the early 1990s but, until very recently, have been overlooked in genomic studies. Here we investigated structural variation in four isolates of the blast fungus M. oryzae from different grass hosts and analyzed the sequences of mini-chromosomes in the rice, foxtail millet and goosegrass isolates. The mini-chromosomes of these isolates turned out to be highly diverse with distinct sequence composition. They are enriched in repetitive elements and have lower gene density than core-chromosomes. We identified several virulence-related genes in the mini-chromosome of the rice isolate, including the virulence-related polyketide synthase Ace1 and two variants of the effector gene AVR-Pik. Macrosynteny analyses around these loci revealed structural rearrangements, including inter-chromosomal translocations between core- and mini-chromosomes. Our findings provide evidence that mini-chromosomes emerge from structural rearrangements and segmental duplication of core-chromosomes and might contribute to adaptive evolution of the blast fungus. 相似文献