Ciliate Protozoa in the Rumen of Sassaby Antelope, Damaliscus lunatus lunatus, Including the Description of a New Species and Form

ABSTRACT. Composition of rumen ciliate fauna in five Zambian, sassaby antelopes was determined. Six genera, 18 species, and four forms were identified. One new species and form, belonging to the subfamily Diplodiniinae, were found, then labeled Ostracodinium damaliscus n. sp. and Diplodinium bubalidis f. aspinosum n. f., respectively. Only ophryoscolecid species were present while isotrichids were absent. Twelve of 18 total species are commmonly found in African antelopes. Three of those 12 species, Entodinium fyferi, Enoploplastron garstangi and Opisthotrichum janus , are only found in African antelopes. Percentage composition was low in the genera ml of rumen fluid, and the average number of ciliate species per host was 17.2.     

Increase in Shedding of Intercellular Adhesion Molecule-1 in Human Malignant Melanoma Cell Lines Treated With Hyperthermia In Vitro

Human malignant melanoma cell lines were found to increase shedding of soluble intercellular adhesion molecule-1 (sICAM-1) into the culture medium when the cells were treated with hyperthermia at 41–43°C for 3–6 hr in vitro. The content of ICAM-1 in the cell lysate was also found to be increased after hyperthermia. The increased rate of ICAM-1 concentration in the cells was at maximum when they were incubated at 41°C for 3 hr. Also, the melanoma cell lines heat-treated at 41°C showed more intense immuno-fluorescence in the ICAM-1 expression on the cell surface. It remains to be investigated further whether the effects of hyperthermia on the ICAM-1 expression in melanoma cells is to augment membrane ICAM-1 expression, which in turn leads to shedding of soluble ICAM-1 or only to acceleration of shedding of sICAM-1 by unknown mechanisms.     

THREE-DIMENSIONAL STRUCTURE OF EXTRACELLULAR MATRIX REVERSIBLY REGULATES MORPHOLOGY,PROLIFERATION AND COLLAGEN METABOLISM OF PERISINUSOIDAL STELLATE CELLS (VITAMIN A-STORING CELLS)

The three-dimensional structure of the extracellular substratum was found to regulate reversibly the morphology, proliferation and collagen synthesis of perisinusoidal stellate cells (lipocytes, i.e. fat-storing ‘Ito’ cells). On non-coated polystyrene and type I collagen-coated culture dishes, the cells spread well and extended their cellular processes. On the surface of type I collagen gels, the cells gathered and formed a mesh-like structure. However, in type I collagen gel where the cells were surrounded by type I collagen three-dimensionally, the cells extended their fine cellular processes and resembled the star-shaped stellate cells seenin vivo. The cell proliferation was more prominent in culture dishes coated with type I collagen or in polystyrene culture dishes than on or in type I collagen gels. The collagen synthesis was affected in the same manner. These data indicate that the nature and the three-dimensional structure of the extracellular matrix (ECM) can regulate morphology, proliferation and functions of the perisinusoidal stellate cells. In order to examine the reversibility of these regulations, we liberated cultured cells with trypsin or with purified bacterial collagenase and re-seeded them onto or into each substratum. The cells changed their shape, rate of proliferation and collagen synthesis according to each new substratum. These results indicate that the three-dimensional structure of ECM reversibly regulates the morphology, proliferation rate and functions of the perisinusoidal stellate cells.     

水稻半矮生基因sd—1的遗传性状表现：Ⅲ.半矮生与穗粒数的结合

半矮生基因ｓｄ－１有抗倒伏和改善株型的效果,广泛存在于世界各地的水稻丰产品种中,最近发现该基因具有减少穗长和千粒重的副作用。为澄清此副作用,能否通过遗传背景的改良得以克服,进而培育成粳稻型的半矮生大穗品咱,本研究用高秆品种农林２９号及其半矮生纯合系ＳＣ－ＴＮ１和大穗品种Ａｋｅｎｏｈｏｓｈｉ的杂种进行了分析。用Ｆ４代系统的实验结果表明,半矮生系统的穗长较高秆系统的为小。但是,由于半矮生系统的枝梗数。     

Ciliate Protozoa in the Rumen of Kafue Lechwe, Kobus leche kafuensis, in Zambia, with the Description of Four New Species

ABSTRACT The composition of the rumen ciliate fauna in 76 Kafue lechwe inhabiting a limited area in Zambia was surveyed and five genera containing 24 species with 16 formae belonging to the family Ophryoscolecidae were identified. Four new species belonging to Diplodiniinae were recognized and described as Diplodinium lochinvarense n. sp., Diplodinium leche n. sp., Diplodinium zambiense n. sp., and Metadinium ossiculi n. sp. In addition, Ostracodinium gracile form fissilaminatum Dogiel, 1932 was found for the second time and described as Metadinium fissilaminatum n. comb. The species composition was fairly unusual. Seven of the species have been found only in African wild antelopes and these species were found more frequently than cosmopolitan species. There was no evidence of isotrichid species. The average density of ciliates per 1 ml of the rumen fluid was 25.7 times 10⁴, and the number of ciliate species per head of host was 10.8.