全文获取类型
收费全文 | 28596篇 |
免费 | 2859篇 |
国内免费 | 16篇 |
出版年
2021年 | 463篇 |
2020年 | 314篇 |
2019年 | 392篇 |
2018年 | 498篇 |
2017年 | 418篇 |
2016年 | 619篇 |
2015年 | 1118篇 |
2014年 | 1160篇 |
2013年 | 1602篇 |
2012年 | 1889篇 |
2011年 | 1901篇 |
2010年 | 1238篇 |
2009年 | 1118篇 |
2008年 | 1571篇 |
2007年 | 1569篇 |
2006年 | 1430篇 |
2005年 | 1476篇 |
2004年 | 1403篇 |
2003年 | 1298篇 |
2002年 | 1326篇 |
2001年 | 365篇 |
2000年 | 344篇 |
1999年 | 386篇 |
1998年 | 349篇 |
1997年 | 250篇 |
1996年 | 212篇 |
1995年 | 234篇 |
1994年 | 222篇 |
1993年 | 221篇 |
1992年 | 273篇 |
1991年 | 240篇 |
1990年 | 245篇 |
1989年 | 248篇 |
1988年 | 277篇 |
1987年 | 220篇 |
1986年 | 202篇 |
1985年 | 253篇 |
1984年 | 219篇 |
1983年 | 217篇 |
1982年 | 278篇 |
1981年 | 249篇 |
1980年 | 201篇 |
1979年 | 182篇 |
1978年 | 204篇 |
1977年 | 175篇 |
1976年 | 176篇 |
1975年 | 156篇 |
1974年 | 168篇 |
1973年 | 139篇 |
1972年 | 131篇 |
排序方式: 共有10000条查询结果,搜索用时 234 毫秒
1.
2.
3.
4.
5.
6.
Russell W. Avramenko Elizabeth M. Redman Roy Lewis Thomas A. Yazwinski James D. Wasmuth John S. Gilleard 《PloS one》2015,10(12)
Parasitic helminth infections have a considerable impact on global human health as well as animal welfare and production. Although co-infection with multiple parasite species within a host is common, there is a dearth of tools with which to study the composition of these complex parasite communities. Helminth species vary in their pathogenicity, epidemiology and drug sensitivity and the interactions that occur between co-infecting species and their hosts are poorly understood. We describe the first application of deep amplicon sequencing to study parasitic nematode communities as well as introduce the concept of the gastro-intestinal “nemabiome”. The approach is analogous to 16S rDNA deep sequencing used to explore microbial communities, but utilizes the nematode ITS-2 rDNA locus instead. Gastro-intestinal parasites of cattle were used to develop the concept, as this host has many well-defined gastro-intestinal nematode species that commonly occur as complex co-infections. Further, the availability of pure mono-parasite populations from experimentally infected cattle allowed us to prepare mock parasite communities to determine, and correct for, species representation biases in the sequence data. We demonstrate that, once these biases have been corrected, accurate relative quantitation of gastro-intestinal parasitic nematode communities in cattle fecal samples can be achieved. We have validated the accuracy of the method applied to field-samples by comparing the results of detailed morphological examination of L3 larvae populations with those of the sequencing assay. The results illustrate the insights that can be gained into the species composition of parasite communities, using grazing cattle in the mid-west USA as an example. However, both the technical approach and the concept of the ‘nemabiome’ have a wide range of potential applications in human and veterinary medicine. These include investigations of host-parasite and parasite-parasite interactions during co-infection, parasite epidemiology, parasite ecology and the response of parasite populations to both drug treatments and control programs. 相似文献
7.
8.
Abstract Two new species of water mites, Pionopsis (s.s.)longicosta sp. nov. and Pionopsis (s.s.) zhaoi sp. nov. are described in the present paper. This is the first record of the genus of the subfamily Tiphyinae in the family Pionidae from China. 相似文献
9.
Joseph P Valentine 《Journal of experimental marine biology and ecology》2003,295(1):63-90
Despite recent rapid increases in the occurrence of nonindigenous marine organisms in the marine environment, few studies have critically examined the invasion process for a marine species. Here we use manipulative experiments to examine processes of invasion for the Asian kelp Undaria pinnatifida (Harvey) Suringar at two sites on the east coast of Tasmania. Disturbance to reduce cover of the native algal canopy was found to be critical in the establishment of U. pinnatifida, while the presence of a stable native algal canopy inhibited invasion. In the first sporophyte growth season following disturbance of the canopy, U. pinnatifida recruited in high densities (up to 19 plants m−2) while remaining rare or absent in un-manipulated plots. The timing of disturbance was also important. U. pinnatifida recruited in higher densities in plots where the native canopy was removed immediately prior to the sporophyte growth season (winter 2000), compared with plots where the canopy was removed 6 months earlier during the period of spore release (spring 1999). Removal of the native canopy also resulted in a significant increase in cover of sediment on the substratum. In the second year following canopy removal, U. pinnatifida abundance declined significantly, associated with a substantial recovery of native canopy-forming species. A feature of the recovery of the native algal canopy was a significant shift in species composition. Species dominant prior to canopy removal showed little if any signs of recovery. The recovery was instead dominated by canopy-forming species that were either rare or absent in the study areas prior to manipulation of the canopy. 相似文献
10.