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Baukova NA Alekseeva SG Sorokoumova GM Selishcheva AA Martynova OM Rogozhkina EA Shvets VI 《Prikladnaia biokhimiia i mikrobiologiia》2002,38(2):183-189
A complex lipid preparation was obtained by extraction of soybean flour with organic solvents. This preparation was shown to include not only phospholipids (major components), but also up to 30% saponins. These compounds were identified by TLC, HPLC, and 1H-NMR spectroscopy. Minor components of the lipid extract were represented by polypeptides associated with phospholipids via electrostatic or hydrophobic forces. 相似文献
2.
Kiselev A. V. Ostapenko O. V. Rogozhkina E. V. Kholod N. S. Seit-Nebi A. S. Baranov A. N. Lesina E. A. Ivashchenko T. E. Sabetskii V. A. Shavlovskii M. M. Rechinsky V. O. Kisselev L. L. Baranov V. S. 《Molecular Biology》2002,36(1):30-33
Nonsense mutations in the dystrophin gene are the cause of Duchenne muscular dystrophy (DMD) in 10–15% of patients. In such an event, one approach to gene therapy for DMD is the use of suppressor tRNAs to overcome the premature termination of translation of the mutant mRNA. We have carried out cotransfection of the HeLa cell culture with constructs containing a suptRNA gene (pcDNA3suptRNA) and a marker LacZ gene (pNTLacZhis) using their polymer VSST-525 complexes. It was found that the number of cells producing -galactosidase depends inversely on the dose of the suptRNA gene. A single in vivo injection of the construct providing for expression of the suptRNAochre gene into mdx mouse muscle resulted in the production of dystrophin in 2.5% of fibers. This suggests that suppressor tRNAs are applicable in gene therapy for hereditary diseases caused by nonsense mutations. 相似文献
3.
Kiselev AV Ostapenko OV Rogozhkina EV Kholod NS Seit Nebi AS Baranov AN Lesina EA Ivashchenko TE Sabetskiĭ VA Shavlovskiĭ MM Rechinskiĭ VO Kiselev LL Baranov VC 《Molekuliarnaia biologiia》2002,36(1):43-47
Nonsense mutations in the dystrophin gene are the cause of Duchenne muscular dystrophy (DMD) in 10-15% of patients. In such an event, one approach to gene therapy for DMD is the use of suppressor tRNAs to overcome the premature termination of translation of the mutant mRNA. We have carried out cotransfection of the HeLa cell culture with constructs containing a suptRNA gene (pcDNA3suptRNA) and a marker LacZ gene (pNTLacZhis) using their polymer VSST-525 complexes. It was found that the number of cells producing beta-galactosidase depends inversely on the dose of the suptRNA gene. A single in vivo injection of the construct providing for expression of the suptRNAochre gene into mdx mouse muscle resulted in the production of dystrophin in 2.5% of fibers. This suggests that suppressor tRNAs are applicable in gene therapy for hereditary diseases caused by nonsense mutations. 相似文献
4.
E. A. Utkina S. V. Antoshina A. A. Selishcheva G. M. Sorokoumova E. A. Rogozhkina V. I. Shvets 《Russian Journal of Bioorganic Chemistry》2004,30(4):385-390
A mixture of isoflavones was obtained by acid hydrolysis of isoflavone glycosides isolated from the products of soybean processing by successive extraction with aqueous acetone and methanol. The homogeneous isoflavones daidzein and genistein were isolated from the aglycone mixture by adsorption chromatography and identified by spectral and chromatographic methods. The effect of both isoflavones on lipid peroxidation of soy phospholipids in multilamellar vesicles was studied at various concentrations. These aglycones were found to inhibit the formation of lipid hydroperoxides and malonic dialdehyde at concentrations as low as 1 mM. 相似文献
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Bechinger B Skladnev DA Ogrel A Li X Rogozhkina EV Ovchinnikova TV O'Neil JD Raap J 《Biochemistry》2001,40(31):9428-9437
The topologies of zervamicin II and alamethicin, labeled with (15)N uniformly, selectively, or specifically, have been investigated by oriented proton-decoupled (15)N solid-state NMR spectroscopy. Whereas at lipid-to-peptide (L/P) ratios of 50 (wt/wt) zervamicin II exhibits transmembrane alignments in 1,2-dicapryl (di-C10:0-PC) and 1,2-dilauroyl (di-C12:0-PC) phosphatidylcholine bilayers, it adopts orientations predominantly parallel to the membrane surface when the lengths of the fatty acyl chains are extended. The orientational order of zervamicin II increases with higher phospholipid concentrations, and considerable line narrowing is obtained in di-C10:0-PC/zervamicin II membranes at L/P ratios of 100 (wt/wt). In contrast to zervamicin, alamethicin is transmembrane throughout most, if not all, of its length when reconstituted into 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayers. The (31)P solid-state NMR spectra of all phospholipid/peptaibol samples investigated show a high degree of headgroup order, indicating that the peptides do not distort the bilayer structure. The observed differences in peptide orientation between zervamicin and alamethicin are discussed with reference to differences in their lengths, helical conformations, distribution of (hydroxy)proline residues, and hydrophobic moments. Possible implications for peptaibol voltage-gating are also described. 相似文献
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Extracellular hydrolases (protease and alkaline phosphatase) of the coccidioidal fungus possessed antigenic properties and caused production of the corresponding antibodies. Phosphatase-antiphosphatase-substrate system apparently has future prospects for the elaboration of immunobiochemical methods for the diagnosis of coccidioidomycosis. 相似文献
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Utkina EA Antoshina SV Selishcheva AA Sorokoumova GM Rogozhkina EA Shvets VI 《Bioorganicheskaia khimiia》2004,30(4):429-435
A mixture of isoflavones was obtained by acid hydrolysis of isoflavone glycosides isolated from the products of soybean processing by a successive extraction with aqueous acetone and methanol. Homogeneous isoflavones genistein and daidzein were isolated from the aglycone mixture by adsorption chromatography and identified by spectral and chromatographic methods. The effect of both isoflavones on lipid peroxidation of soy phospholipids in multilamellar vesicles was studied at various concentrations. These aglycones were found to inhibit the formation of lipid hydroperoxides and malonic dialdehyde at the concentrations as low as 1 mM. 相似文献
8.
N P Khrapova N M Rogozhkina S F Zharkova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1988,(8):90-94
A highly effective and specific solid-phase enzyme immunoassay system has been developed. The enzyme immunoassay is a highly sensitive technique for the detection and identification of C. immitis cellular and metabolic antigens. This technique is suitable for the study of strain differences in the antigenic composition of C. immitis, rendered harmless by different methods. The expediency of the preliminary sonification of cell suspensions of C. immitis, the causative agent of coccidioidomycosis, has been experimentally confirmed. 相似文献
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Baukova N. A. Alekseeva S. G. Sorokoumova G. M. Selishcheva A. A. Martynova O. M. Rogozhkina E. A. Shvets V. I. 《Applied Biochemistry and Microbiology》2002,38(2):159-164
A complex lipid preparation was obtained by extracting soybean flour with organic solvents. This preparation was shown to include not only phospholipids (major components), but also 30% saponins. These compounds were identified by TLC, HPLC, and 1H-NMR spectroscopy. Minor components of the lipid extract were represented by polypeptides bound to phospholipids by electrostatic or hydrophobic forces. 相似文献
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