全文获取类型
收费全文 | 530253篇 |
免费 | 52146篇 |
国内免费 | 261篇 |
出版年
2018年 | 9746篇 |
2017年 | 9557篇 |
2016年 | 9413篇 |
2015年 | 7811篇 |
2014年 | 8943篇 |
2013年 | 13269篇 |
2012年 | 16679篇 |
2011年 | 21126篇 |
2010年 | 15259篇 |
2009年 | 14301篇 |
2008年 | 18480篇 |
2007年 | 20047篇 |
2006年 | 13140篇 |
2005年 | 13272篇 |
2004年 | 12856篇 |
2003年 | 12633篇 |
2002年 | 12227篇 |
2001年 | 22312篇 |
2000年 | 22352篇 |
1999年 | 17839篇 |
1998年 | 6088篇 |
1997年 | 6332篇 |
1996年 | 6049篇 |
1995年 | 5653篇 |
1994年 | 5752篇 |
1993年 | 5652篇 |
1992年 | 14801篇 |
1991年 | 14275篇 |
1990年 | 14177篇 |
1989年 | 13949篇 |
1988年 | 12794篇 |
1987年 | 12159篇 |
1986年 | 11274篇 |
1985年 | 11316篇 |
1984年 | 9220篇 |
1983年 | 8027篇 |
1982年 | 6055篇 |
1981年 | 5382篇 |
1980年 | 5177篇 |
1979年 | 8840篇 |
1978年 | 6866篇 |
1977年 | 6123篇 |
1976年 | 5926篇 |
1975年 | 6517篇 |
1974年 | 6831篇 |
1973年 | 6817篇 |
1972年 | 6668篇 |
1971年 | 5893篇 |
1970年 | 4818篇 |
1969年 | 4562篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
András Szilágyi István Scheuring David P. Edwards Jerome Orivel Douglas W. Yu 《Ecology letters》2009,12(12):1306-1316
Theory suggests that spatial structuring should select for intermediate levels of virulence in parasites, but empirical tests are rare and have never been conducted with castration (sterilizing) parasites. To test this theory in a natural landscape, we construct a spatially explicit model of the symbiosis between the ant-plant Cordia nodosa and its two, protecting ant symbionts, Allomerus and Azteca . Allomerus is also a castration parasite, preventing fruiting to increase colony fecundity. Limiting the dispersal of Allomerus and host plant selects for intermediate castration virulence. Increasing the frequency of the mutualist, Azteca , selects for higher castration virulence in Allomerus , because seeds from Azteca -inhabited plants are a public good that Allomerus exploits. These results are consistent with field observations and, to our knowledge, provide the first empirical evidence supporting the hypothesis that spatial structure can reduce castration virulence and the first such evidence in a natural landscape for either mortality or castration virulence. 相似文献
4.
E. Azizi A. R. Deslauriers N. C. Holt C. E. Eaton 《Biomechanics and modeling in mechanobiology》2017,16(5):1633-1643
The collagenous extracellular matrix (ECM) of skeletal muscle functions to transmit force, protect sensitive structures, and generate passive tension to resist stretch. The mechanical properties of the ECM change with age, atrophy, and neuromuscular pathologies, resulting in an increase in the relative amount of collagen and an increase in stiffness. Although numerous studies have focused on the effect of muscle fibrosis on passive muscle stiffness, few have examined how these structural changes may compromise contractile performance. Here we combine a mathematical model and experimental manipulations to examine how changes in the mechanical properties of the ECM constrain the ability of muscle fibers and fascicles to radially expand and how such a constraint may limit active muscle shortening. We model the mechanical interaction between a contracting muscle and the ECM using a constant volume, pressurized, fiber-wound cylinder. Our model shows that as the proportion of a muscle cross section made up of ECM increases, the muscle’s ability to expand radially is compromised, which in turn restricts muscle shortening. In our experiments, we use a physical constraint placed around the muscle to restrict radial expansion during a contraction. Our experimental results are consistent with model predictions and show that muscles restricted from radial expansion undergo less shortening and generate less mechanical work under identical loads and stimulation conditions. This work highlights the intimate mechanical interaction between contractile and connective tissue structures within skeletal muscle and shows how a deviation from a healthy, well-tuned relationship can compromise performance. 相似文献
5.
In the present work we studied the effect of antioxidants of the SkQ1 family (10-(6′-plastoquinonyl)decyltriphenylphosphonium) on the oxidative hemolysis of erythrocytes induced by a lipophilic free radical initiator 2,2′-azobis(2,4-dimethylvaleronitrile) (AMVN) and a water-soluble free radical initiator 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH). SkQ1 was found to protect erythrocytes from hemolysis, 2 μM being the optimal concentration. Both the oxidized and reduced SkQ1 forms exhibited protective properties. Both forms of SkQ1 also inhibited lipid peroxidation in erythrocytes induced by the lipophilic free radical initiator AMVN as detected by accumulation of malondialdehyde. However, in the case of induction of erythrocyte oxidation by AAPH, the accumulation of malondialdehyde was not inhibited by SkQ1. In the case of AAPH-induced hemolysis, the rhodamine-containing analog SkQR1 exerted a comparable protective effect at the concentration of 0.2 μM. At higher SkQ1 and SkQR1 concentrations, the protective effect was smaller, which was attributed to the ability of these compounds to facilitate hemolysis in the absence of oxidative stress. We found that plastoquinone in the oxidized form of SkQ1 could be reduced by erythrocytes, which apparently accounted for its protective action. Thus, the protective effect of SkQ in erythrocytes, which lack mitochondria, proceeded at concentrations that are two to three orders of magnitude higher than those that were active in isolated mitochondria. 相似文献
6.
Anna-Kristina Marel Matthias Zorn Christoph Klingner Roland Wedlich-S?ldner Erwin Frey Joachim?O. R?dler 《Biophysical journal》2014,107(5):1054-1064
Collective migration of mechanically coupled cell layers is a notable feature of wound healing, embryonic development, and cancer progression. In confluent epithelial sheets, the dynamics have been found to be highly heterogeneous, exhibiting spontaneous formation of swirls, long-range correlations, and glass-like dynamic arrest as a function of cell density. In contrast, the flow-like properties of one-sided cell-sheet expansion in confining geometries are not well understood. Here, we studied the short- and long-term flow of Madin-Darby canine kidney (MDCK) cells as they moved through microchannels. Using single-cell tracking and particle image velocimetry (PIV), we found that a defined averaged stationary cell current emerged that exhibited a velocity gradient in the direction of migration and a plug-flow-like profile across the advancing sheet. The observed flow velocity can be decomposed into a constant term of directed cell migration and a diffusion-like contribution that increases with density gradient. The diffusive component is consistent with the cell-density profile and front propagation speed predicted by the Fisher-Kolmogorov equation. To connect diffusion-mediated transport to underlying cellular motility, we studied single-cell trajectories and occurrence of vorticity. We discovered that the directed large-scale cell flow altered fluctuations in cellular motion at short length scales: vorticity maps showed a reduced frequency of swirl formation in channel flow compared with resting sheets of equal cell density. Furthermore, under flow, single-cell trajectories showed persistent long-range, random-walk behavior superimposed on drift, whereas cells in resting tissue did not show significant displacements with respect to neighboring cells. Our work thus suggests that active cell migration manifests itself in an underlying, spatially uniform drift as well as in randomized bursts of short-range correlated motion that lead to a diffusion-mediated transport. 相似文献
7.
8.
9.
10.