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ABSTRACTCollagen-derived dipeptide prolyl hydroxyproline (Pro-Hyp) is involved in the proliferation and differentiation of various types of cultured cells. To elucidate the mechanism underlying Pro-Hyp actions during osteoblast differentiation, we hypothesized that proteins binding to Pro-Hyp serve to mediate cellular signaling, affecting Runx2 expression. Recently, we performed the characterization of Foxg1, that it enhances Runx2 expression in the presence of Pro-Hyp. Our findings indicate that Pro-Hyp directly binds to the Foxg1 recombinant protein, which leads to the structural alteration of the Foxg1 protein. In addition, Foxg1 appears to interact with Runx2 in the absence of Pro-Hyp, with Pro-Hyp disrupting the interaction between Foxg1 and Runx2. Collectively, our results indicate that the Pro-Hyp bound Foxg1 alters the structured conformation of Foxg1, resulting in conformational changes that lead to dissociation from Runx2. These novel findings suggest that during osteoblast differentiation, Pro-Hyp mediates Runx2 activity though directly binding to Foxg1 and increases Runx2 expression.Abbreviations: CPT: collagen peptide; GST: Glutathione S-transferase; PAGE: Polyacrylamide gel electrophoresis; PCR: Polymerase chain reaction; prolyl hydroxyproline: Pro-Hyp 相似文献
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Masayuki Sato Gaku Takahashi Shigehiro Shibata Makoto Onodera Yasushi Suzuki Yoshihiro Inoue Shigeatsu Endo 《PloS one》2015,10(12)
We previously reported that a soluble CD14-subtype (sCD14-ST) immunochromatographic test (ICT) for plasma is more convenient than chemiluminescent enzyme immunoassay (CLEIA), but plasma separation makes bedside measurements difficult. We developed a new sCD14-ST ICT for whole blood and investigated whether quantitative determinations of sCD14-ST by ICT were useful for diagnosing sepsis and severe sepsis/septic shock. We studied 20 patients who fulfilled two or more systemic inflammatory response syndrome (SIRS) criteria and 32 patients who had been diagnosed with sepsis or severe sepsis/septic shock. Whole blood was collected on day 0 (on admission) and day 7, and the sCD14-ST concentration was quantitatively measured by CLEIA and ICT for whole blood. The patients’ Acute Physiology and Chronic Health Evaluation (APACHE) II, Sequential Organ Failure Assessment (SOFA), and Mortality in Emergency Department Sepsis (MEDS) scores were also calculated. The cut-off values obtained by the quantitative measurements made by ICT were 464.5 pg/mL for sepsis and 762.7 pg/mL for severe sepsis/septic shock (P < 0.0001). A Bland–Altman plot showed that no fixed bias or proportional bias was detected between CLEIA and quantitative ICT for whole blood. sCD14-ST concentrations were significantly correlated with APACHE II, SOFA, and MEDS scores (P < 0.0001). These results suggest that the new sCD14-ST ICT for whole blood may be a useful tool for the convenient, rapid bedside diagnosis and treatment of sepsis. 相似文献
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Ridwansyah Iwan Yulianti Meti Apip Onodera Shin-ichi Shimizu Yuta Wibowo Hendro Fakhrudin M. 《Limnology》2020,21(3):487-498
Limnology - The Cimanuk River with a total watershed area of 4010.8 km2 flowing from the Garut Regency to Indramayu Delta is the longest in West Java Province. However, the cumulative... 相似文献
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Natsumi Takeyama Yasuhisa Ano Guoying Wu Nobuyoshi Kubota Keiichi Saeki Akikazu Sakudo Eiichi Momotani Katsuaki Sugiura Masayoshi Yukawa Takashi Onodera 《Life sciences》2009,84(19-20):678-687
AimsInsulinoma-associated protein 2 (IA-2) is a member of the protein tyrosine phosphatase family that is localized on the insulin granule membrane. IA-2 is also well known as one of the major autoantigens in Type 1 diabetes mellitus. IA-2 gene deficient mice were recently established and showed abnormalities in insulin secretion. Thus, detailed localization of IA-2 was studied using wild-type and IA-2 gene deficient mice.Main methodsTo localize IA-2 expression in mouse neuroendocrine tissues, monoclonal antibodies were generated against IA-2 and western blot and immunohistochemical analyses were carried out in IA-2+/+ mice. IA-2?/? mice served as a negative control.Key findingsWestern blot analysis revealed that the 65 kDa form of IA-2 was observed in the cerebrum, cerebellum, medulla oblongata, pancreas, adrenal gland, pituitary gland, muscular layers of the stomach, small intestine, and colon. By immunohistochemical analysis, IA-2 was produced in endocrine cells in pancreatic islets, adrenal medullary cells, thyroid C-cells, Kulchitsky cells, and anterior, intermediate, and posterior pituitary cells. In addition, IA-2 was found in somatostatin-producing D-cells and other small populations of cells were scattered in the gastric corpus. IA-2 expression in neurites was confirmed by the immunostaining of IA-2 using primary cultured neurons from the small intestine and nerve growth factor (NGF)-differentiated PC12 cells.SignificanceThe IA-2 distribution in peripheral neurons appeared more intensely in neurites rather than in the cell bodies. 相似文献
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Kenji Nomiya Kuniaki Onodera Ken Tsukagoshi Kouhei Shimada Akira Yoshizawa Tada-aki Itoyanagi Akiyoshi Sugie Shinichiro Tsuruta Ryo Sato Noriko Chikaraishi Kasuga 《Inorganica chimica acta》2009,362(1):43-55
Metal-oxygen bonding complexes (M = MgII, MnII, NiII, MoVI, WVI, PdII, SbIII, BiIII, FeIII, TiIV, KI, BaII, ZrIV and HfIV) with a hinokitiol (Hhino; 2-hydroxy-4-isopropylcyclohepta-2,4,6-trienone or β-thujaplicin) ligand, which has two unequivalent oxygen donor atoms, were synthesized and characterized by elemental analysis, TG/DTA, FT-IR and solution (1H and 13C) NMR spectroscopy. Single-crystal X-ray structure analysis revealed various molecular structures for the complexes, which were classified into several families of family, i.e. type A [MII(hino)2(L)]2 (M = MgII, MnII, NiII; L = EtOH or MeOH), with a dimeric structure consisting of one bridging hino− anion, one chelating hino− anion and one alcohol or water molecule, type B, with the octahedral, cis-dioxo, bis-chelate complexes cis-[MVIO2(hino)2] (M = MoVI, WVI), type C, with square planar complex [MII(hino)2] (M = PdII), type D, with tris-chelate, 7-coordinate complexes with one inert electron pair [MIII(hino)3] (M = SbIII, BiIII), type D′, with the bis-chelate, pseudo-6-coordinate complexes with one inert electron pair [MIII(hino)2X] (M = SbIII, X = Br), type E, with tris-chelate, 6-coordinate complexes with Δ and Λ isomers [MIII(hino)3] (M = FeIII), type E′ of bis-chelate, 6-coordinate complex [MIV(hino)2X2] (M = TiIV, X = Cl), type F, with water-soluble alkali metal salts [MI(hino)] (M = KI), and type H, with tetrakis-chelate, 8-coordinate complexes [MIV(hino)4](M = ZrIV, HfIV). These structural features were compared with those of metal complexes with a related ligand, tropolone (Htrop). The antimicrobial activities of these complexes, evaluated in terms of minimum inhibitory concentration (MIC; μg mL−1) in two systems, were compared to elucidate the relationship between structure and antimicrobial activity. 相似文献