全文获取类型
收费全文 | 132篇 |
免费 | 14篇 |
国内免费 | 1篇 |
出版年
2023年 | 3篇 |
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 6篇 |
2014年 | 11篇 |
2013年 | 11篇 |
2012年 | 9篇 |
2011年 | 6篇 |
2010年 | 4篇 |
2008年 | 6篇 |
2007年 | 6篇 |
2006年 | 8篇 |
2005年 | 14篇 |
2004年 | 7篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1994年 | 6篇 |
1993年 | 1篇 |
1991年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
排序方式: 共有147条查询结果,搜索用时 125 毫秒
1.
La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. 总被引:43,自引:22,他引:21 下载免费PDF全文
K Meerovitch Y V Svitkin H S Lee F Lejbkowicz D J Kenan E K Chan V I Agol J D Keene N Sonenberg 《Journal of virology》1993,67(7):3798-3807
Translation initiation on poliovirus RNA occurs by internal binding of ribosomes to a sequence within the 5' untranslated region. We have previously characterized a HeLa cell protein, p52, that binds to a fragment of the poliovirus 5' untranslated region (K. Meerovitch, J. Pelletier, and N. Sonenberg, Genes Dev. 3:1026-1034, 1989). Here we report the purification of the HeLa p52. Protein microsequencing identified p52 as La autoantigen. The La protein is a human antigen that is recognized by antibodies from patients with autoimmune disorders such as systemic lupus erythematosus and Sjögren's syndrome. We show that the La protein stimulates translation of poliovirus RNA, but not brome mosaic virus, tobacco mosaic virus, and alfalfa mosaic virus 4 RNA, translation in a reticulocyte lysate. In addition, La corrects aberrant translation of poliovirus RNA in a reticulocyte lysate. Subcellular immunolocalization showed that La protein is mainly nuclear, but after poliovirus infection, La is redistributed to the cytoplasm. Our results suggest that La protein is involved in poliovirus internal initiation of translation and might function through a similar mechanism in the translation of cellular mRNAs. 相似文献
2.
Terzin Marko Paletta Maria Grazia Matterson Kenan Coppari Martina Bavestrello Giorgio Abbiati Marco Bo Marzia Costantini Federica 《Coral reefs (Online)》2021,40(4):1391-1391
Coral Reefs - A correction to this paper has been published: https://doi.org/10.1007/s00338-021-02120-y 相似文献
3.
Kenan Gumustekin Mehmet Ciftci Abdulkadir Coban Sayit Altikat Omer Aktas Mustafa Gul 《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):497-502
Effects of nicotine, and nicotine + vitamin E on glucose 6-phosphate dehydrogenase (G-6PD) activity in rat muscle, heart, lungs, testicle, kidney, stomach, brain and liver were investigated in vivo and in vitro on partially purified homogenates. Supplementation period was 3 weeks (n = 8 rats per group): nicotine [0.5 mg/kg/day, intraperitoneal (ip)]; nicotine + vitamin E [75 mg/kg/day, intragastric (ig)]; and control group (receiving only vehicle). The results showed that nicotine (0.5 mg/kg, ip) inhibited G-6PD activity in the lungs, testicle, kidney, stomach and brain by 12.5% (p < 0.001), 48% (p < 0.001), 20.8% (p < 0.001), 13% (p < 0.001) and 23.35% (p < 0.001) respectively, and nicotine had no effects on the muscle, heart and liver G6PD activity. Also, nicotine + vitamin E inhibited G-6PD activity in the testicle, brain, and liver by 32.5% (p < 0.001), 21.5% (p < 0.001), and 16.5% (p < 0.001) respectively, and nicotine + vitamin E activated the muscle, and stomach G-6PD activity by 36% (p < 0.05), and 20% (p < 0.001) respectively. In addition, nicotine + vitamin E did not have any effects on the heart, lungs, and kidney G-6PD activity. In addition, in vitro studies were also carried out to elucidate the effects of nicotine and vitamin E on G-6PD activity, which correlated well with in vivo experimental results in lungs, testicles, kidney, stomach, brain and liver tissues. These results show that vitamin E administration generally restores the inactivation of G-6PD activity due to nicotine administration in various rat tissues in vivo, and also in vitro. 相似文献
4.
5.
6.
Cyclic AMP is sufficient for triggering the exocytic recruitment of aquaporin-2 in renal epithelial cells 总被引:1,自引:0,他引:1
The initial response of renal epithelial cells to the antidiuretic hormone arginine vasopressin (AVP) is an increase in cyclic AMP. By applying immunofluorescence, cell membrane capacitance and transepithelial water flux measurements we show that cAMP alone is sufficient to elicit the antidiuretic cellular response in primary cultured epithelial cells from renal inner medulla, namely the transport of aquaporin-2 (AQP2)-bearing vesicles to, and their subsequent fusion with, the plasma membrane (AQP2 shuttle). The AQP2 shuttle is evoked neither by AVP-independent Ca2+ increases nor by AVP-induced Ca2+ increases. However, clamping cytosolic Ca2+ concentrations below resting levels at 25 nM inhibited exocytosis. Exocytosis was confined to a slow monophasic response, and readily releasable vesicles were missing. Analysis of endocytic capacitance steps revealed that cAMP does not decelerate the retrieval of AQP2 from the plasma membrane. Our data suggest that cAMP initiates an early step, namely the transport of AQP2-bearing vesicles towards the plasma membrane, and do not support a regulatory function for Ca2+ in the AQP2 shuttle. 相似文献
7.
The lambda Gam protein inhibits RecBCD binding to dsDNA ends 总被引:1,自引:0,他引:1
Murphy KC 《Journal of molecular biology》2007,371(1):19-24
Inactivation of the Escherichia coli RecBCD enzyme by the lambda Gam protein is an essential step that accompanies the lambda Red proteins for gene replacement using recombineering technology. It has been shown that Gam inhibits all the activities of RecBCD to the same extent. Nonetheless, some in vivo properties of recBCD mutants cannot be mimicked effectively by the expression of gam in vivo. An examination of the mechanism of Gam's inhibition of RecBCD was performed, and it was found that Gam inhibits the binding of RecBCD to double-stranded DNA ends, even if RecBCD is bound to DNA before its interaction with Gam. When ATP is added to the reaction to induce helicase activity, most of the reaction is inhibited by Gam, but residual amounts of unwinding are detected, despite a 40-fold excess of Gam/RecBCD. The same inhibitory effect of Gam was seen on RecBCD that had been modified by the P22 anti-RecBCD protein Abc2, though the inhibitory effect was diminished due to the tighter binding of Abc2-modified RecBCD to double-stranded DNA ends. These data suggest that cells containing Gam-expressing plasmids retain a small amount of uninhibited enzyme. Given the suspected instability of Gam in vivo, care must be taken when interpreting results from experiments containing Gam-inhibited RecBCD species. A revised model is proposed for Gam-induced radioresistance of E. coli to ionizing radiation. 相似文献
8.
Leard JS Cirillo MA Katsnelson E Kimiatek DA Miller TW Trebincevic K Garbalosa JC 《Journal of strength and conditioning research / National Strength & Conditioning Association》2007,21(4):1296-1299
Vertical jump height is frequently used by coaches, health care professionals, and strength and conditioning professionals to objectively measure function. The purpose of this study is to determine the concurrent validity of the jump and reach method (Vertec) and the contact mat method (Just Jump) in assessing vertical jump height when compared with the criterion reference 3-camera motion analysis system. Thirty-nine college students, 25 females and 14 males between the ages of 18 and 25 (mean age 20.65 years), were instructed to perform the countermovement jump. Reflective markers were placed at the base of the individual's sacrum for the 3-camera motion analysis system to measure vertical jump height. The subject was then instructed to stand on the Just Jump mat beneath the Vertec and perform the jump. Measurements were recorded from each of the 3 systems simultaneously for each jump. The Pearson r statistic between the video and the jump and reach (Vertec) was 0.906. The Pearson r between the video and contact mat (Just Jump) was 0.967. Both correlations were significant at the 0.01 level. Analysis of variance showed a significant difference among the 3 means F(2,235) = 5.51, p < 0.05. The post hoc analysis showed a significant difference between the criterion reference (M = 0.4369 m) and the Vertec (M = 0.3937 m, p = 0.005) but not between the criterion reference and the Just Jump system (M = 0.4420 m, p = 0.972). The Just Jump method of measuring vertical jump height is a valid measure when compared with the 3-camera system. The Vertec was found to have a high correlation with the criterion reference, but the mean differed significantly. This study indicates that a higher degree of confidence is warranted when comparing Just Jump results with a 3-camera system study. 相似文献
9.
Best T Li D Skol AD Kirchhoff T Jackson SA Yasui Y Bhatia S Strong LC Domchek SM Nathanson KL Olopade OI Huang RS Mack TM Conti DV Offit K Cozen W Robison LL Onel K 《Nature medicine》2011,17(8):941-943
Survivors of pediatric Hodgkin's lymphoma are at risk for radiation therapy-induced second malignant neoplasms (SMNs). We identified two variants at chromosome 6q21 associated with SMNs in survivors of Hodgkin's lymphoma treated with radiation therapy as children but not as adults. The variants comprise a risk locus associated with decreased basal expression of PRDM1 (encoding PR domain containing 1, with ZNF domain) and impaired induction of the PRDM1 protein after radiation exposure. These data suggest a new gene-exposure interaction that may implicate PRDM1 in the etiology of radiation therapy-induced SMNs. 相似文献
10.