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排序方式: 共有307条查询结果,搜索用时 17 毫秒
1.
Irene Pila-Castellanos Diana Molino Joe McKellar Laetitia Lines Juliane Da Graca Marine Tauziet Laurent Chanteloup Ivan Mikaelian Laurne Meyniel-Schicklin Patrice Codogno Jacky Vonderscher Cdric Delevoye Olivier Moncorg Eric Meldrum Caroline Goujon Etienne Morel Benoit de Chassey 《PLoS pathogens》2021,17(2)
Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza virus entry, host cells experience modifications of endomembranes, including those used for virus trafficking and replication. Here we report that influenza virus infection modifies mitochondrial morphodynamics by promoting mitochondria elongation and altering endoplasmic reticulum-mitochondria tethering in host cells. Expression of the viral RNA recapitulates these modifications inside cells. Virus induced mitochondria hyper-elongation was promoted by fission associated protein DRP1 relocalization to the cytosol, enhancing a pro-fusion status. We show that altering mitochondrial hyper-fusion with Mito-C, a novel pro-fission compound, not only restores mitochondrial morphodynamics and endoplasmic reticulum-mitochondria contact sites but also dramatically reduces influenza replication. Finally, we demonstrate that the observed Mito-C antiviral property is directly connected with the innate immunity signaling RIG-I complex at mitochondria. Our data highlight the importance of a functional interchange between mitochondrial morphodynamics and innate immunity machineries in the context of influenza viral infection. 相似文献
2.
Jacky G. Goetz 《Cell Adhesion & Migration》2012,6(3):190-192
Nastiness of cancer does not only reside in the corruption of cancer cells by genetic aberrations that drive their sustained proliferative power—the roots of malignancy—but also in its aptitude to reciprocally sculpt its surrounding environment and cellular stromal ecosystem, in such a way that the corrupted tumor microenvironment becomes a full pro-tumorigenic entity. Such a contribution had been appreciated three decades ago already, with the discovery of tumor angiogenesis and extracellular matrix remodeling. Nevertheless, the recent emergence of the tumor microenvironment as the critical determinant in cancer biology is paralleled by the promising therapeutic potential it carries, opening alternate routes to fight cancer. The study of the tumor microenvironment recruited numerous lead-scientists over the years, with distinct perspectives, and some of them have kindly accepted to contribute to the elaboration of this special issue entitled Tumor microenvironment indoctrination: An emerging hallmark of cancer. 相似文献
3.
Jacky L. Snoep Mark R. de Graef Adrie H. Westphal Arie de Kok M. Joost Teixeira de Mattos Oense M. Neijssel 《FEMS microbiology letters》1993,114(3):279-283
Abstract The effect of NADH on the activity of the purified pyruvate dehydrogenase complexes (PDHc) of Enterococcus (Ec.) faecalis, Lactococcus lactis, Azotobacter vinelandii and Escherichia coli was determined in vitro. It was found that the PDHc of E. coli and L. lactis was active only at relatively low NADH/NAD ratios, whereas the PDHc of Ec. faecalis was inhibited only at high NADH/NAD ratios. The PDHc of Azotobacter vinelandii showed an intermediate sensitivity. The organisms were grown in chemostat culture under conditions that led to different intracellular NADH/NAD ratios and the PDHc activities in vivo could be calculated from the specific rates of product formation. Under anaerobic growth conditions, only Ec. faecelis expressed PDHc activity in vivo. The activities in vivo of the complexes of the different organisms were in good agreement with their properties determined in vitro. The physiological consequences of these results are discussed. 相似文献
4.
Jacky Michel 《Aerobiologia》1990,6(1):54-57
Summary A technical approach, in terms of air quality, implies the review of all components within the air conditioning system and
the check of their characteristics, as far as contamination sources are concerned. The contribution introduces the parameters
to be examined and specifies the importance of validation. 相似文献
5.
6.
Julie Neury‐Ormanni Caroline Doose Nabil Majdi Jacky Vedrenne Soizic Morin Sebastian Hss Walter Traunspurger 《Invertebrate Biology》2019,138(4)
The neonicotinoid imidacloprid and the herbicide diuron are long‐lived pesticides commonly detected in European rivers. Both have lethal as well as sublethal effects on aquatic invertebrates dwelling in streambeds. Here, we performed lethality tests of imidacloprid and diuron on seven species of widespread, free‐living nematodes and the model organism Caenorhabditis elegans. Our results indicated that nematodes were relatively tolerant to both pesticides, and only two species (Diploscapter coronatus and Plectus opisthocirculus) showed mortality at high nominal concentrations of imidacloprid (119 mg/L) and diuron (33 mg/L). The changes observed in nematode community structure after imidacloprid and diuron exposure may have been related to trade‐offs between sensitivity to toxicants and changes in competitive abilities of the species. While the former can be tested using single‐species tests, we recommend that the latter be tested in further experiments using multispecies communities. Our results suggest that the presence of these pesticides could favor nematodes over other meiofaunal groups found in freshwater sediments. 相似文献
7.
Frantz Alain C. Viglino Andrea Wilwert Elodie Cruz Ana-Paula Wittische Julian Weigand Alexander M. Buijk Jacky Nyssen Pierrette Dekeukeleire Daan Dekker Jasja J.A. Horsburgh Gavin J. Schneider Simone Lang Mara Caniglia Romolo Galaverni Marco Schleimer Anna Bücs Szilárd-Lehel Pir Jacques B. 《Biodiversity and Conservation》2022,31(3):925-948
Biodiversity and Conservation - In the European Union, all bat species are strictly protected and member states must ensure their conservation. However, if populations are genetically structured,... 相似文献
8.
Vincent Hyenne Ahmet Apaydin David Rodriguez Coralie Spiegelhalter Sarah Hoff-Yoessle Maxime Diem Saurabh Tak Olivier Lefebvre Yannick Schwab Jacky G. Goetz Michel Labouesse 《The Journal of cell biology》2015,211(1):27-37
Exosomes are secreted vesicles arising from the fusion of multivesicular bodies (MVBs) with the plasma membrane. Despite their importance in various processes, the molecular mechanisms controlling their formation and release remain unclear. Using nematodes and mammary tumor cells, we show that Ral GTPases are involved in exosome biogenesis. In Caenorhabditis elegans, RAL-1 localizes at the surface of secretory MVBs. A quantitative electron microscopy analysis of RAL-1–deficient animals revealed that RAL-1 is involved in both MVB formation and their fusion with the plasma membrane. These functions do not involve the exocyst complex, a common Ral guanosine triphosphatase (GTPase) effector. Furthermore, we show that the target membrane SNARE protein SYX-5 colocalizes with a constitutively active form of RAL-1 at the plasma membrane, and MVBs accumulate under the plasma membrane when SYX-5 is absent. In mammals, RalA and RalB are both required for the secretion of exosome-like vesicles in cultured cells. Therefore, Ral GTPases represent new regulators of MVB formation and exosome release. 相似文献
9.
Guoxing Wang Boaz J. van Driel Gongxian Liao Michael S. O’Keeffe Peter J. Halibozek Jacky Flipse Burcu Yigit Veronica Azcutia Francis W. Luscinskas Ninghai Wang Cox Terhorst 《PloS one》2015,10(3)
Previous studies have demonstrated that the cell surface receptor Slamf1 (CD150) is requisite for optimal NADPH-oxidase (Nox2) dependent reactive oxygen species (ROS) production by phagocytes in response to Gram- bacteria. By contrast, Slamf8 (CD353) is a negative regulator of ROS in response to Gram+ and Gram- bacteria. Employing in vivo migration after skin sensitization, induction of peritonitis, and repopulation of the small intestine demonstrates that in vivo migration of Slamf1-/- dendritic cells and macrophages is reduced, as compared to wt mice. By contrast, in vivo migration of Slamf8-/- dendritic cells, macrophages and neutrophils is accelerated. These opposing effects of Slamf1 and Slamf8 are cell-intrinsic as judged by in vitro migration in transwell chambers in response to CCL19, CCL21 or CSF-1. Importantly, inhibiting ROS production of Slamf8-/- macrophages by diphenyleneiodonium chloride blocks this in vitro migration. We conclude that Slamf1 and Slamf8 govern ROS–dependent innate immune responses of myeloid cells, thus modulating migration of these cells during inflammation in an opposing manner. 相似文献
10.
The effect of reactive oxygen species on motility parameters,DNA integrity,tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpio L.) spermatozoa 下载免费PDF全文