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Frantz Alain C. Viglino Andrea Wilwert Elodie Cruz Ana-Paula Wittische Julian Weigand Alexander M. Buijk Jacky Nyssen Pierrette Dekeukeleire Daan Dekker Jasja J.A. Horsburgh Gavin J. Schneider Simone Lang Mara Caniglia Romolo Galaverni Marco Schleimer Anna Bücs Szilárd-Lehel Pir Jacques B. 《Biodiversity and Conservation》2022,31(3):925-948
Biodiversity and Conservation - In the European Union, all bat species are strictly protected and member states must ensure their conservation. However, if populations are genetically structured,... 相似文献
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Glucosylation activity and complex formation of two classes of reversibly glycosylated polypeptides 下载免费PDF全文
Langeveld SM Vennik M Kottenhagen M Van Wijk R Buijk A Kijne JW de Pater S 《Plant physiology》2002,129(1):278-289
Reversibly glycosylated polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. In plants, these proteins may function, for example, in cell wall synthesis and/or in synthesis of starch. We have isolated wheat (Triticum aestivum) and rice (Oryza sativa) Rgp cDNA clones to study the function of RGPs. Sequence comparisons showed the existence of two classes of RGP proteins, designated RGP1 and RGP2. Glucosylation activity of RGP1 and RGP2 from wheat and rice was studied. After separate expression of Rgp1 and Rgp2 in Escherichia coli or yeast (Saccharomyces cerevisiae), only RGP1 showed self-glucosylation. In Superose 12 fractions from wheat endosperm extract, a polypeptide with a molecular mass of about 40 kD is glucosylated by UDP-glucose. Transgenic tobacco (Nicotiana tabacum) plants, overexpressing either wheat Rgp1 or Rgp2, were generated. Subsequent glucosylation assays revealed that in RGP1-containing tobacco extracts as well as in RGP2-containing tobacco extracts UDP-glucose is incorporated, indicating that an RGP2-containing complex is active. Gel filtration experiments with wheat endosperm extracts and extracts from transgenic tobacco plants, overexpressing either wheat Rgp1 or Rgp2, showed the presence of RGP1 and RGP2 in high-molecular mass complexes. Yeast two-hybrid studies indicated that RGP1 and RGP2 form homo- and heterodimers. Screening of a cDNA library using the yeast two-hybrid system and purification of the complex by an antibody affinity column did not reveal the presence of other proteins in the RGP complexes. Taken together, these results suggest the presence of active RGP1 and RGP2 homo- and heteromultimers in wheat endosperm. 相似文献
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