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T N Ul'masov M K Gulov K A Aliev V M Andrianov E S Piruzian 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1989,(8):17-20
The fragments of cotton Gossipium hirsutum c.v. 108-f chloroplast genome were cloned in Escherichia coli cells. The cloned psbA and rbcL genes have been selected using the heterologous probes from spinach. The preliminary attempts to clone the complete psbA gene in pUC19 vector failed, probably, due to the toxicity of its product to Escherichia coli cells, and its 5'- and 3'-ends were cloned separately. Reconstruction experiments revealed that while the complete psbA gene was unable to be stably inherited by Escherichia coli cells, its structural part lacking the promoter region could be readily cloned in the bacterial cells. 相似文献
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Mohammed Mamdani Vernell Williamson Gowon O. McMichael Tana Blevins Fazil Aliev Amy Adkins Laura Hack Tim Bigdeli Andrew D. van der Vaart Bradley Todd Web Silviu-Alin Bacanu Gursharan Kalsi COGA Consortium Kenneth S. Kendler Michael F. Miles Danielle Dick Brien P. Riley Catherine Dumur Vladimir I. Vladimirov 《PloS one》2015,10(9)
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Brecht Devleesschauwer Juanita A. Haagsma Frederick J. Angulo David C. Bellinger Dana Cole D?rte D?pfer Aamir Fazil Eric M. Fèvre Herman J. Gibb Tine Hald Martyn D. Kirk Robin J. Lake Charline Maertens de Noordhout Colin D. Mathers Scott A. McDonald Sara M. Pires Niko Speybroeck M. Kate Thomas Paul R. Torgerson Felicia Wu Arie H. Havelaar Nicolas Praet 《PloS one》2015,10(12)
Background
The Foodborne Disease Burden Epidemiology Reference Group (FERG) was established in 2007 by the World Health Organization to estimate the global burden of foodborne diseases (FBDs). This paper describes the methodological framework developed by FERG''s Computational Task Force to transform epidemiological information into FBD burden estimates.Methods and Findings
The global and regional burden of 31 FBDs was quantified, along with limited estimates for 5 other FBDs, using Disability-Adjusted Life Years in a hazard- and incidence-based approach. To accomplish this task, the following workflow was defined: outline of disease models and collection of epidemiological data; design and completion of a database template; development of an imputation model; identification of disability weights; probabilistic burden assessment; and estimating the proportion of the disease burden by each hazard that is attributable to exposure by food (i.e., source attribution). All computations were performed in R and the different functions were compiled in the R package ''FERG''. Traceability and transparency were ensured by sharing results and methods in an interactive way with all FERG members throughout the process.Conclusions
We developed a comprehensive framework for estimating the global burden of FBDs, in which methodological simplicity and transparency were key elements. All the tools developed have been made available and can be translated into a user-friendly national toolkit for studying and monitoring food safety at the local level. 相似文献6.
Michael J. Garner Carolee Carson Erika J. Lingohr Aamir Fazil Victoria L. Edge Jan Trumble Waddell 《PloS one》2015,10(4)
Background
Antimicrobial resistance (AMR) of infectious agents is a growing concern for public health organizations. Given the complexity of this issue and how widespread the problem has become, resources are often insufficient to address all concerns, thus prioritization of AMR pathogens is essential for the optimal allocation of risk management attention. Since the epidemiology of AMR pathogens differs between countries, country-specific assessments are important for the determination of national priorities.Objective
To develop a systematic and transparent approach to AMR risk prioritization in Canada.Methods
Relevant AMR pathogens in Canada were selected through a transparent multi-step consensus process (n=32). Each pathogen was assessed using ten criteria: incidence, mortality, case-fatality, communicability, treatability, clinical impact, public/political attention, ten-year projection of incidence, economic impact, and preventability. For each pathogen, each criterion was assigned a numerical score of 0, 1, or 2, and multiplied by criteria-specific weighting determined through researcher consensus of importance. The scores for each AMR pathogen were summed and ranked by total score, where a higher score indicated greater importance. A sensitivity analysis was conducted to determine the effects of changing the criteria-specific weights.Results
The AMR pathogen with the highest total weighted score was extended spectrum B-lactamase-producing (ESBL) Enterobacteriaceae (score=77). When grouped by percentile, ESBL Enterobacteriaceae, Clostridium difficile, carbapenem-resistant Enterobacteriaceae, and methicillin-resistant Staphylococcus aureus were in the 80-100th percentile.Conclusion
This assessment provides useful information for prioritising public health strategies regarding AMR resistance at the national level in Canada. As the AMR environment and challenges change over time and space, this systematic and transparent approach can be adapted for use by other stakeholders domestically and internationally. Given the complexity of influences, resource availability and multiple stakeholders, regular consideration of AMR activities in the public health realm is essential for appropriate and responsible prioritisation of risk management that optimises the health and security of the population. 相似文献7.
Emelyanova M. A. Amossenko F. A. Semyanikhina A. V. Aliev V. A. Barsukov Yu. A. Lyubchenko L. N. Nasedkina T. V. 《Molecular Biology》2015,49(4):550-559
Molecular Biology - Somatic mutations of KRAS, PIK3CA, and BRAF cause insensitivity of colorectal tumors to therapy with anti-EGFR monoclonal antibodies, necessitating a genetic testing prior to... 相似文献
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Panina A. A. Toporova V. A. Rybchenko V. S. Balabashin D. S. Argentova V. V. Yakimov S. A. Solopova O. N. Aliev T. K. Dolgikh D. A. Sveshnikov P. G. Kirpichnikov M. P. 《Russian Journal of Bioorganic Chemistry》2020,46(4):572-581
Russian Journal of Bioorganic Chemistry - The development of new therapies for malignant tumors is an urgent task. Currently, the humanized antibody trastuzumab is considered the “gold... 相似文献
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Rybchenko V. S. Balabashin D. S. Panina A. A. Solopova O. N. Yakimov S. A. Aliev T. K. Dolgikh D. A. Kirpichnikov M. P. 《Russian Journal of Bioorganic Chemistry》2020,46(3):327-333
Russian Journal of Bioorganic Chemistry - The extracellular part of the ErbB2 receptor (ecdErbB2), an oncological marker and a target for therapeutic antibodies, has been expressed in eukaryotic... 相似文献
10.
Rybchenko V. S. Panina A. A. Novoseletsky N. V. Balabashin D. S. Yakimov S. A. Solopova O. N. Sveshnikov P. G. Aliev T. K. Dolgikh D. A. Kirpichnikov M. P. 《Russian Journal of Bioorganic Chemistry》2020,46(5):778-786
Russian Journal of Bioorganic Chemistry - Humanization of antibodies for the development of novel therapeutic agents with low immunogenicity remains a topical problem in modern science. In the... 相似文献