全文获取类型
收费全文 | 10273篇 |
免费 | 981篇 |
国内免费 | 1519篇 |
出版年
2024年 | 12篇 |
2023年 | 156篇 |
2022年 | 188篇 |
2021年 | 524篇 |
2020年 | 424篇 |
2019年 | 523篇 |
2018年 | 512篇 |
2017年 | 375篇 |
2016年 | 469篇 |
2015年 | 772篇 |
2014年 | 834篇 |
2013年 | 837篇 |
2012年 | 1034篇 |
2011年 | 967篇 |
2010年 | 609篇 |
2009年 | 567篇 |
2008年 | 640篇 |
2007年 | 561篇 |
2006年 | 494篇 |
2005年 | 426篇 |
2004年 | 386篇 |
2003年 | 299篇 |
2002年 | 240篇 |
2001年 | 115篇 |
2000年 | 126篇 |
1999年 | 122篇 |
1998年 | 91篇 |
1997年 | 100篇 |
1996年 | 80篇 |
1995年 | 64篇 |
1994年 | 48篇 |
1993年 | 28篇 |
1992年 | 23篇 |
1991年 | 15篇 |
1990年 | 15篇 |
1989年 | 11篇 |
1988年 | 11篇 |
1987年 | 13篇 |
1986年 | 2篇 |
1985年 | 9篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 14篇 |
1979年 | 2篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1950年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
N. J. Lassam Zheng Lin Michael G. Shennan Anouk Courseaux Bin T. Teh Patrick Gaudray Catharina Larsson 《Human genetics》1997,99(6):776-780
MLK-3 kinase is a widely expressed serine/ threonine kinase that bears multiple protein interaction domains and regulates
signals mediated by the stress-responsive pathway. Thus, MLK-3 signaling affects numerous cellular processes, raising the
possibility that MLK-3 might play a role in oncogenesis. In this report, we describe the fine mapping of the MLK-3 gene within the 11q13.1 chromosomal region. By integrating data from somatic cell hybrids and double color fluorescence in
situ hybridization on metaphase chromosomes and DNA fibers, MLK-3 has been assigned approximately 1 Mb telomeric of PYGM, close to the D11S546 locus. Since the MEN1 susceptibility locus is also located within the 11q13.1 region, we have carried
out Southern and Northern blot analyses, as well as protein truncation assays to establish whether abnormalities in MLK-3 lead to the development of this familial cancer syndrome. Our observations exclude MLK-3 as the MEN1 gene.
Received: 25 September 1996 / Revised: 16 December 1996 相似文献
5.
Yao Shen Yueyang Tian Xiaojie Shi Jianbo Yang Li Ouyang Jieqiong Gao Jianxin Lu 《Cell biochemistry and function》2014,32(6):530-537
Astrocytes play a key role in removing the synaptically released glutamate from the extracellular space and maintaining the glutamate below neurotoxic level in the brain. However, high concentration of glutamate leads to toxicity in astrocytes, and the underlying mechanisms are unclear. The purpose of this study was to investigate whether energy metabolism disorder, especially impairment of mitochondrial respiration, is involved in the glutamate‐induced gliotoxicity. Exposure to 10‐mM glutamate for 48 h stimulated glycolysis and respiration in astrocytes. However, the increased oxygen consumption was used for proton leak and non‐mitochondrial respiration, but not for oxidative phosphorylation and ATP generation. When the exposure time extended to 72 h, glycolysis was still activated for ATP generation, but the mitochondrial ATP‐linked respiration of astrocytes was reduced. The glutamate‐induced astrocyte damage can be mimicked by the non‐metabolized substrate d ‐aspartate but reversed by the non‐selective glutamate transporter inhibitor TBOA. In addition, the glutamate toxicity can be partially reversed by vitamin E. These findings demonstrate that changes of bioenergetic profile occur in cultured cortical astrocytes exposed to high concentration of glutamate and highlight the role of mitochondria respiration in glutamate‐induced gliotoxicity in cortical astrocytes. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
6.
7.
Julie M. Koeman Ryan C. Russell Min-Han Tan David Petillo Michael Westphal Katherine Koelzer Julie L. Metcalf Zhongfa Zhang Daisuke Matsuda Karl J. Dykema Heather L. Houseman Eric J. Kort Laura L. Furge Richard J. Kahnoski Stphane Richard Annick Vieillefond Pamela J. Swiatek Bin Tean Teh Michael Ohh Kyle A. Furge 《PLoS genetics》2008,4(9)
8.
9.
Hao Qing Liu Xiaoguang Zhao Guozhong Jiang Lu Li Ming Zeng Bin 《Biotechnology letters》2016,38(3):519-525
Biotechnology Letters - To characterize biochemically the lipid metabolism-regulating acyl-CoA binding protein (ACBP) from the industrially-important fungus Aspergillus oryzae. A full-length cDNA... 相似文献
10.
Many animals exhibit different behaviors in different seasons. The photoperiod can have effects on migration, breeding, fur growth, and other processes. The cyclic growth of the fur and feathers of some species of mammals and birds, respectively, is stimulated by the photoperiod as a result of hormone-dependent regulation of the nervous system. To further examine this phenomenon, we evaluated the Arbas Cashmere goat (Capra hircus), a species that is often used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclic cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Assessments of tissue sections indicated that the short photoperiod significantly induced cashmere growth. This conclusion was supported by a comparison of the differences in gene expression between the short photoperiod and natural conditions using gene chip technology. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and these genes were found to be involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Knowledge about differences in the expression of these genes as well as their functions and periodic regulation patterns increases our understanding of Cashmere goat hair follicle growth. This study also provides preliminary data that may be useful for the development of an artificial method to improve cashmere production by controlling the light cycle, which has practical significance for livestock breeding. 相似文献