脑室注射6-羟多巴胺对黄鼠冬眠入眠的影响

采用脑室内注射化学去交感药物6-羟多巴胺(6-OHDA)的方法,观察了人为地降低脑内去甲肾上腺素(NE)系统活动对达乌尔黄鼠入眠的影响。结果表明:脑室注射100-200μg6-OHDA使脑内NE含量减少50％以上,明显促进黄鼠入眠,平均入眠诱导期比自然冬眠动物明显缩短,整个冬眠季内冬眠时间延长,冬眠黄鼠仍具有正常的入眠觉醒周期。这些结果提示脑内NE系统活动水平降低是触发动物入眠的重要因素之一。     

脑室注射6-OHDA对黄鼠脑内单胺类神经递质与β-内啡肽和血清TSH含量影响

本文研究了脑室注射6—OHDA对达乌尔黄鼠脑内单胺类递质和β内啡肽,血清TSH,T3和T4含量的影响:1)注射后7天内,单胺类含量随剂量和注射后的天数而改变。2)下丘脑,丘脑,垂体和海马β内啡肽含量在注射后第3天显著升高,第5天,第7天逐渐回降,但仍高于对照。3)血清TSH,T3,T4含量在注射后3—5天内显著低于对照。4)冬眠动物垂体,下丘脑和海马β内啡肽含量比对照显著增高。本文为脑室注射6—OHDA促进黄鼠冬眠的生理学机制提供了一些依据。     

脑室注射6—羟多巴胺对黄鼠科眠入眠的影响

The effect of forced depletion of brain norepinephrine (NE) on the onset of hibernation was observed in the ground squirrel (Citellus dauricus) by intraventricular injection of 6-hydroxydopamine (6-OHDA). The results showed: (1) Intraventricular injection of 100-200 micrograms 6-OHDA, which depleted 50-60% NE, markedly facilitated the onset of hibernation, i.e. the average induction period for hibernation in the treated animals was significantly shorter than that of the natural hibernating animals. (2) The average total torpor time in the treated animals was longer than that of natural hibernating animals. (3) All hibernating animals treated with 6-OHDA were able to wake up from deep hibernation spontaneously and undergo normal hibernation bouts. The results indicate the decrease of NE system activity in brain is one important factor in triggering the onset of hibernation.     

腹腔注射对氯苯丙氨酸对达乌尔黄鼠冬眠的影响

本工作采用腹腔注射5-羟色胺合成抑制剂对氯苯丙氨酸降低脑内5-HT含量的方法,观察了脑内5-HT系统在达乌尔黄鼠自然冬眠和脑室注射6-羟多巴胺促进入眠效应中的作用。结果表明;在自然情况下,达乌尔黄鼠入眠后脑内5-HT含量增加,腹腔注射对氯苯丙氨酸后动物入眠诱导期明显延长;在脑室注射6-羟多巴胺人为地降低脑内NE系统活动的条件下,脑内5-HT含量即使处于较低水平,动物也能很快入眠,但入眠时脑内5-HT含量需要恢复到一定的基础水平。提示脑内NE系统功能活动的降低或/和5-HT系统功能活动加强同是触发动物入眠的重要因素。周期性入眠和觉醒可能依赖于脑内这两个系统功能活动的平衡。