不同培养基和三种金属离子对桦褐孔菌培养菌丝体的羊毛甾醇和麦角甾醇积累的影响

Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4?7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35?mol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I. obliquus.     

不同培养基和三种金属离子对桦褐孔菌培养菌丝体的羊毛甾醇和麦角甾醇积累的影响

Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study, different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and MgSO4·7H2O (0.05%) with pH level at 6.5 yielded a maximum production of the two sterols, which can further be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I.     

瑞舒伐他汀钙与非诺贝特联合治疗血脂异常的疗效及对肝肾功能的影响

目的:观察瑞舒伐他汀钙与非诺贝特联合治疗血脂异常的疗效及对肝肾功能的影响。方法:选择2017年1月至2019年6月间我院门诊收治的127例血脂异常患者,随机分为研究组(n=63)和对照组(n=64),在针对合并症给予对症治疗的基础上,对照组采用瑞舒伐他汀钙治疗,研究组在对照组基础上联合非诺贝特治疗。观察两组血脂水平、肝肾功能、临床效果以及不良反应发生率。结果:治疗后,两组总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)水平低于治疗前(P<0.05),且研究组低于对照组(P<0.05);高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)水平均高于治疗前(P<0.05),且研究组高于对照组(P<0.05);两组治疗前后尿素氮(UN)、肌酐(Cr)比较差异无统计学意义(P>0.05);研究组的总有效率为92.06%(58/63),高于对照组的79.69%(51/64),差异有统计学意义(P<0.05);研究组不良反应发生率为11.11%(7/63),稍高于对照组的9.38%(6/64),但差异无统计学意义(P>0.05)。结论:在瑞舒伐他汀钙基础上联合非诺贝特治疗血脂异常可提高临床有效率,可增强调节血脂的作用,其安全性较好,但对血脂异常患者的肝功能影响较大,临床可视患者情况合理选用用药方案。