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With confocal microscopy, we recorded calcium transients and analyzed calcium removal rate at different temperatures in cardiac myocytes from the rat, a non-hibernator, and the ground squirrel, a hibernator. The results showed a remarkable increase of the diastolic level of calcium transients in the rat but no detectable change in the ground squirrel. Calcium transient of the ground squirrel, compared with that of the rat at the same temperature, had a shorter duration and showed a faster calcium removal. As indicated by the pharmacological effect of cyclopiazonic acid, calcium uptake by sarcoplasmic reticulum (SR) was the major mechanism of calcium removal, and was faster in the ground squirrel than in the rat. Our results confirmed the essential role of SR in hypothermia-tolerant adaptation, and negated the importance of Na-Ca exchange. We postulated the possibility to improve hypothermia-tolerance of the cardiac tissue of non-hibernating mammals. 相似文献
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摘要 目的:探讨适形调强放疗联合顺铂为基础的化疗治疗≥5 cm非小细胞肺癌(NSCLC)的疗效及对生存质量和血清肿瘤标志物的影响。方法:选取2016年1月~2019年1月来我院就诊的60例NSCLC患者(肿瘤直径≥5 cm)。分组方法采用随机数字表法,分为对照组(适形调强放疗)和观察组(适形调强放疗联合顺铂为基础的化疗治疗),各30例。对比两组疗效、生存质量、1年生存率、血清肿瘤标志物和不良反应发生情况。结果:观察组治疗后的生存质量优于对照组(P<0.05)。观察组治疗后的总有效率为63.33%(19/30),高于对照组的36.67%(11/30)(P<0.05)。治疗后,两组神经元烯醇化酶(NSE)、糖类抗原199(CA199)、细胞角蛋白19片段(CYFRA21-1)较治疗前下降,且观察组低于对照组(P<0.05)。观察组的1年生存率66.67%高于对照组36.67%(P<0.05)。两组放射性肺炎、放射性食管炎的发生率组间对比无差异(P>0.05)。观察组的全身不良反应、血液毒性发生率高于对照组(P<0.05)。结论:适形调强放疗联合顺铂为基础的化疗治疗≥5 cm NSCLC患者,疗效较好,可有效控制疾病进展,提高患者生存率。 相似文献
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共聚焦显微术测定大鼠和黄鼠心肌细胞游离Ca2+浓度与温度的关系 总被引:1,自引:0,他引:1
运用共聚焦激光扫描显微成像术对比研究非冬眠动物大鼠和冬眠动物黄鼠心肌细胞胞内Ca2+浓度 ([Ca2+]i) 随温度的变化.首先标定了不同温度下Ca2+探针indo-1的解离常数,提出并证明按α定态设定标定溶液pH值的必要性.细胞荧光分析显示,大鼠心肌细胞[Ca2+]i随温度降低显著上升,低温下频繁出现自发钙波,胞内发生钙超载;相比较冬眠动物黄鼠心肌细胞[Ca2+]i在相同条件下保持稳定,避免发生钙超载.认识其中的钙稳态机制可能对有关医学问题有潜在的指导意义. 相似文献
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The temperature-dependence of infraeeliular free caleimn (Ca) was investigated in mdo-1 loaded ventricular myocytes from the ral, a non-hibernator, and from the ground squirrel, a hibernator. The dissociation constant of indo-l at different temperatures was calibrated both al pll-tat and at @-stat . and the result demonstrated that the @-stat ralibration should be prettrred . Analysis of the fluoreseent image showed a striking increase of Ca2 as well as spontaneous caleiuni waves in ral cells, indicating an overloaded cakuum. In contrast, cardiac myocytes of the ground sqnirraf were found to keep a constant (Ca2 ) without caleium overload regardless of temperature variation. It is be-lieved that understanding of the mechanisms underlying the interccllular caleima homeostasis of hibrernators may lead to solutions of some medical questions . 相似文献
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运用共聚焦激光扫描显微成像术对比研究非冬眠动物大鼠和冬眠动物黄鼠心肌细胞胞内Ca2+浓度 ( [Ca2+]i)随温度的变化 .首先标定了不同温度下Ca2+探针indo 1的解离常数 ,提出并证明按α定态设定标定溶液pH值的必要性 .细胞荧光分析显示 ,大鼠心肌细胞 [Ca2+ ]i 随温度降低显著上升 ,低温下频繁出现自发钙波 ,胞内发生钙超载 ;相比较冬眠动物黄鼠心肌细胞[Ca2+ ]i 在相同条件下保持稳定 ,避免发生钙超载 .认识其中的钙稳态机制可能对有关医学问题有潜在的指导意义 . 相似文献
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