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Purified DNAs from Chicken Embryo Fibroblast (CEF) cultures infected with MDV strain Rispens were used as templates. Specific fragment with the size of about 2.9 kb was successfully amplified through Polymerase Chain Reaction(PCR) and identified to be gB gene of MDV by dot blot hybridization with a digoxigenin-labelled MDV gB specific oligonucleotide probe. The gB gene from strain Rispens was cloned into pUC19 and FPV insertion vector pFG1175-1 to construct plasmid pMGB and pFGBR1775-1 respectively. DOSPER liposome-mediated transfection with insertion vector DNA pFGBR1175-1 was performed on CEF monolayers infected with FPV 3-4 h earlier. Recombinant FPV was clone purified. Immunofluorescence Assay(IFA) showed that MDV gB gene had been expressed in FPV. 相似文献
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改造猪的器官移植给人类被认为是解决人类移植器官供不应求的可能方案,但由于猪和人在免疫学上的差异使移植到人体的猪的带血管器官很快被排斥掉,本文综述了近十年来对猪-人之间最重要的差异性抗原表位的研究进展. 相似文献
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生物高分辨电子显微学是近年来发展起来的一种可与X射线晶体学相媲美的测定生物大分子高分辨结构的方法.它克服了一些限制X射线晶体学应用的困难,可以直接对非晶体状态的生物大分子或仅能形成二维晶体的蛋白进行结构测定.这一技术主要包括高分辨电子显微象的获得与电子显微象解析.文章就这一技术应用中的一些问题:自然结构的保持、辐射损伤、低衬度、低信噪比等进行了讨论. 相似文献
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