番茄交替氧化酶基因的克隆和表达

利用简并PCR扩增产物做探针筛选番茄cDNA基因文库获得一个全长交替氧化酶cDNA基因LeAoxlau.经序列分析得出,该基因全长1 418bp,编码区序列长1 077 bp,编码约40 kD的前体蛋白.该蛋白在转运到线粒体时被加工成32kD的成熟蛋白.Southern印迹杂交分析结果显示该基因以单拷贝形式存在于番茄的基因组中RT-PCR显示,该基因在在番茄植株的根、茎、叶和子叶中表达.重组表达实验表明该基因能在大肠杆菌中表达.     

番木瓜环斑病毒PCR检测技术研究

建立了检测番木瓜环斑病毒(PRV)的免疫捕捉-PCR(IC-PCR)法、ELISA-PCR法和巢式-PCR法,它们分别能从10-4、10-7和10-14稀释度的番木瓜叶粗汁液(所含鲜叶组织的量分别0.5ug、0.5ng和5×10-6ng)中检测出PRV.     

植物病毒株系间交互保护和遗传工程交互保护

本文简述了植物抗病毒基因工程的研究进展,通过比较植物病毒株系间交互保护和遗传工程交互保护现象及二者可能的作用机理,提出了在基因工程中为获得较高抗性的抗病毒转基因植物必须选用株系中序列同源率高的株系进行基因克隆必要性。     

番木瓜环斑病毒PCR检测技术研究

建立了检测番木瓜环斑病毒 (PRV)的免疫捕捉 PCR (IC PCR)法、ELISA PCR法和巢式 PCR法 ,它们分别能从 10 - 4、10 - 7和 10 - 14 稀释度的番木瓜叶粗汁液 (所含鲜叶组织的量分别 0 .5ug、0 .5ng和 5× 10 - 6ng)中检测出PRV。     

黄瓜花叶病毒衣壳蛋白基因转化辣椒研究

The plant expression vector of the coat protein(CP) gene of cucumber mosaic virus (CMV) BS strain was used to transform three kinds of pepper (Capsicum annuum) tissues (cotyledon, stem and root) by agrobacterium-mediated co-cultivation. 53%-68.4% of the total tissues (639) can be induced to be calli, but only cotyledon calli can be further regenerated to form shoots (regenerated efficiency 39.7%). 70%(42/60) of the putative transformed plants were confirmed to have CP gene in their genomes by Southern blot. The mRNAs and the CP were respectively found in 80% of transgenic plants by Northern blot and DAS-ELISA. 24 of the transgenic plants expressing CP gene of BS strain showed three kinds of resistant level (severe symptom, delay of symptomatic development, no symptom) to infection of CMV-BS and of CMV-P. However, there was distinctly higher resistance to inoculation of CMV-BS than that with CMV-P in these transgenic plants.