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| 番茄交替氧化酶基因的克隆和表达 | |
| 引用本文： | 宋从凤,Broth wayne,王金生,胡晋生.番茄交替氧化酶基因的克隆和表达[J].植物生理与分子生物学学报,2004,30(5):503-510. |
| 作者姓名： | 宋从凤 Broth wayne 王金生 胡晋生 |
| 作者单位： | 1. 南京农业大学植保学院,农业部植物病虫害检测和治理重点实验室,南京,210095,中国;夏威夷大学植物和环境保护学系,檀香山,96822,美国 2. 夏威夷大学植物和环境保护学系,檀香山,96822,美国 3. 南京农业大学植保学院,农业部植物病虫害检测和治理重点实验室,南京,210095,中国 |
| 基金项目： | 太平洋地区项目管理组，美国农部CSRS特别基金 |
| 摘 要： | 利用简并PCR扩增产物做探针筛选番茄cDNA基因文库获得一个全长交替氧化酶cDNA基因LeAoxlau.经序列分析得出,该基因全长1 418bp,编码区序列长1 077 bp,编码约40 kD的前体蛋白.该蛋白在转运到线粒体时被加工成32kD的成熟蛋白.Southern印迹杂交分析结果显示该基因以单拷贝形式存在于番茄的基因组中RT-PCR显示,该基因在在番茄植株的根、茎、叶和子叶中表达.重组表达实验表明该基因能在大肠杆菌中表达. |
| 关 键 词： | 番茄 交替氧化酶基因 克隆 基因表达 |
Cloning and Expression of an Alternative Oxidase Gene from Lycopersicon esculentum |
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| Broth wayne.Cloning and Expression of an Alternative Oxidase Gene from Lycopersicon esculentum[J].Journal Of Plant Physiology and Molecular Biology,2004,30(5):503-510. | |
| Authors: | Broth wayne |
| Abstract: | A full-length cDNA gene(LeAoxlau) was isolated from a cDNA library made from ripening fruit of tomato 'UC-82B'(Lycopersicon esculentum), after probing with alternative oxidase(AOX) gene fragments, obtained by degenerate primer PCR. Sequence analysis showed that LeAoxlau was 1 418 bp long and contained a 1 077-bp open reading frame encoding a about 40 kD precursor protein which is processed to a mature protein of 32 kD. Southern blot analysis suggested LeAoxlau is present as a single copy in the genome of tomato. RT-PCR analysis indicated LeAoxlau was expressed in roots, stems, leaves and cotyledons of tomato plants. A recombinant construct containing the open reading frame sequence of the LeAoxlau was transformed into Escherichia coli to express the alternative oxidase precursor protein. |
| Keywords: | tomato alternative oxidase gene clone gene expression |
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