Conservation of ribosomal protein gene ordering in 16 complete genomes

The organization of ribosomal proteins in 16 prokaryotic genomes was studied as an example of comparative genome analyses of gene systems. Hypothetical ribosomal protein-containing operons were constructed. These operons also contained putative genes and other non-ribosomal genes. The correspondences among these genes across different organisms were clarified by sequence homology computations. In this way a cross tabulation of 70 ribosomal proteins genes was constructed. On average, these were organized into 9-14 operons in each genome. There were also 25 non-ribosomal or putative genes in these mainly ribosomal protein operons. Hence the table contains 95 genes in total. It was found that: (i) the conservation of the block of about 20 r-proteins in the L3 and L4 operons across almost the entire eubacteria and ar-chaebacteria is remarkable; (ii) some operons only belong to eubacteria or archaebacte-ria; (iii) although the ribosomal protein operons are highly conserved within domain, there are fine variat     

16个完整基因组中核糖体蛋白基因排列顺序保守性的研究

在16个完整基因组中,对70个核糖体蛋白基因的排列顺序进行了分析.这些基因在每个基因组中平均构成9～14个操纵子.结果显示:(1)L3和L14操纵子中包含的20多个核糖体蛋白的排列顺序在古细菌和真细菌这两个不同界的基因组中都非常保守;(2)有些操纵子结构分别是真细菌或古细菌所特有的;(3)在每一界中,有些操纵子中的核糖体蛋白的基因排列顺序在不同的物种中存在一定的差异,这种差异可以用来推测物种之间的亲缘关系.这种方法为研究古老物种的起源和进化提供了一条新途径.