XeCl（308nm）准分子紫外激光对生物大分子酵母甘露聚糖分子结构的辐照效应

激光在农业和医疗等方面已有广泛应用,并且取得增产和一定疗效,但也有费介现象(暂称后继效应或潜在效应).激光辐照生物体虽然是局部的有限时间,但对由多种生物大分子组成的牛物体的影响可能是综合的.为了探索激光生物学效应的分子机理,以便进一步认识和掌握激光的生物学效应,对激光直接辐照有生物活性的生物大分子一酵母甘露聚糖产生的效应进行电镜扫描观察和紫外及红外光谱法分析研究.实验结果表明:308 nm 激光(单脉冲25 mJ,33.3 ml-34.4 ml,脉冲频率2次/s)无论是固定辐照时间(45 s)变化样晶所置距离,或反之固定样品所置距离(290 mm)改变辐照时广开J为15 s、30 s、45 s或60 s,都能引起受照样品的结构(构象)变化,特别足糖分子结构中富含的-OH、-CH-OH、-C-O-c-、-N-C=0、-C=0等结构部件处的IR谱线对激光能最变化影响敏感.它们既是糖分子内或分子间形成氧键和糖苷键的结构基础部件,又是甘露聚糖(Man-nan)糖基化其他生物大分子进而影响它们在生物信息流中作用的重要结构部件.结果显示糖在激光牛物效应中起了不可忽视的作用,这为综合探索激光生物效应分子机理和糖在生物信息传递中的作用提供了重要线索,为选择甘露聚糖作为生物导弹载体提供了参考依据.     

低强度He-Ne激光辐照人体外周血对淋巴细胞染色体影响的研究

本文通过低强度 Ｈｅ Ｎｅ 激光以能量密度分别为 １４３１ Ｊ／ｃｍ ２ （辐照 ５′）、２８６２ Ｊ／ｃｍ ２ （辐照 １０′）、５７２４ Ｊ／ｃｍ ２ （辐照 ２０′）１１４５２ Ｊ／ｃｍ ２ （辐照 ４０′）照射人体外周血后,检测其淋巴细胞染色体畸变率（ Ｃ Ａ）,激光照射血样（能量密度由低到高）及未照射血样 Ｃ Ａ 分别平均为 ４２９‰、３９６‰、３８１‰、３５９‰、４１９‰, Ｘ２ 检验无显著差异（ Ｐ＞ ００５）,说明低强度的 Ｈｅ Ｎｅ 激光辐照人体细胞对细胞染色体无致畸变效应。而且随着能量密度的增大,染色体的畸变率有降低的趋势,因此认为是低强度的 Ｈｅ Ｎｅ 激光促使细胞内分子的相互作用和能量转换,从而使染色体损伤的修复增强,其机理有待于进一步的研究。     

CO2激光治疗子宫内膜异位症的临床分析

采用ＣＯ２激光对１２例分布于宫颈、阴道及外阴的子宫内膜异位症病人进行病灶切除碳化治疗,并对其随访了３￣７年、无１例复发、局部留下瘢痕。认为外阴子宫内膜异位症采用激光治疗可靠、简便、更优于传统手术治疗。     

Synthesis of a novel series of L-isoserine derivatives as aminopeptidase N inhibitors

A series of novel L-isoserine derivatives were synthesised and evaluated for their ability to inhibit aminopeptidase N (APN)/CD13. In our preliminary biological results, some of these compounds possessed a potent inhibitory activity against the APN. Within this series, compound 14b not only showed similar enzyme inhibition (IC?? of 12.2 μM) compared with the positive control bestatin (half maximal inhibitory concentration (IC??) of 7.3 μM), but also had a potent antiproliferative activity against human cancer cell lines cells.     

柑桔近缘植物酯酶同工酶的研究

本文用聚丙烯酰胺凝胶电泳测定了柑桔近缘植物14个种群的种子及幼苗的酯酶同工酶,根据酶谱及扫描图的异同,分析了彼此的亲缘关系,试验结果表明,柑桔近缘植物种属间的酯酶同工酶的酶带数目,酶活性,迁移率及酶谱扫描均有不同程度的差异,同一品种不同发育时期的同工酶也具有不同表现形式,特别是柑桔种子的酯酶同工酶谱一般较稳定,可以作为柑桔亲缘关系的生化遗传指标。     

Visualization and Characterization of High-Order Chromatin Fibers under Light Microscope during Interphase and Mitotic Stages in Plants

Using genomic in situ hybridization with genomic DNA, high-order chromatin fibers were successfully exhibited under a light microscope through the cell cycle in barley, rice, maize and field bean. From the interphase to prophase and metaphase of mitosis, the fibers were basically similar. Each was estimated to be around 200 nm in diameter, but the strength of signals was not the same along the fiber length. Through the cell cycle a series of dynamic distribution changes occurred in the fibers. In the interphase, they were unraveled. At the early prophase they were arranged with parallel and mirror symmetry. During late-prophase and metaphase, the fibers were bundled and became different visible chromosomes. The parallel coiling and mirror symmetry structures were visible clearly until the metaphase. In anaphase they disappeared. During telophase, in peripheral regions of congregated chromosome group, borderlines of the chromosomes disappeared and the fibers were unraveled. This demonstrated that mitotic chromosomes are assembled and organized by parallel and adjacent coiling of the fibers and the fibers should be the highest order structure for DNA coiling.     

进化论对社会和科学发展的影响

本文概述了进化论的发展和其现代生物学依据,指出进化论对社会、科学发展的重大影响;重点论证了自然选择条件下的竞争机制的生物学意义及其与市场条件下的竞争机制之间的相似性;指出竞争机制是解决资源的有效分配及生存和发展的最好机制;指出采用竞争机制是我国二十多年来经济腾飞的决定性因素之一,竞争机制是大自然的启示,是必由之路。     

低强度HeNe激光辐照人体外周血对淋巴细胞染色体影响的研究

本文通过低强度Ｈｅ－Ｎｅ激光以能量密度分别为１４．３１Ｊ／ｃｍ＾２（辐照５’）、２８．６２Ｊ／ｃｍ＾２（辐照１０）、５７．２４Ｊ／ｃｍ＾２（辐照２０）１１４．５２Ｊ／ｃｍ＾２（辐照４０）燠夫体外周血后,检测其淋染色畸变率（ＣＡ）,激光照射血样（能量密度由低到高）未照射血样ＣＡ分别平均为４．２９‰、３．９６‰、３．８１‰、３．５９０‰、４．１９‰、Ｘ＾检测无显著差异,说明低强度的Ｈｅ－Ｎｅ激光辐照人     

YAG激光锥切治疗顽固性宫颈糜烂伴肥大

我们对１８例顽固性宫颈糜烂伴肥大病人采用ＹＡＧ激光作锥形切除治疗,经１～１０年随访,未见复发,效果满意,激光治疗顽固性宫颈糜烂行锥切优于传统手术切除。