抗果蝇Dxl6蛋白抗体的制备及特异性分析

为了研究果蝇中SR蛋白家族新成员Dxl6的功能,通过RT-PCR得到Dxl6的全长cDNA,并根据Dxl6基因产物的功能区,分别将Dxl6中间区域（Dxl6 middle part, Dxl6MP）、Dxl6 C末端RS结构域（Dxl6 RS domain, Dxl6RSD）序列亚克隆至pGEX-4T-1(His)6C 及pET32a 表达载体中,表达和纯化获得融合蛋白。用纯化的融合蛋白GST-Dxl6RSD-His和GST-Dxl6MP-His免疫家兔,分别得到抗Dxl6RSD和抗Dxl6MP两种抗体。WESTERN BLOT结果显示两种抗体能特异地识别在原核表达系统内表达的抗原,抗Dxl6RSD的抗体对果蝇组织中的Dxl6具有较高的特异性。Abstract: In order to study the function of Dxl6 which is a novel member of SR protein family, its cDNA was cloned by RT-PCR, and the sequences of its RS domain and its middle part were subcloned into two fusion express vectors, pGEX-4T-1His（6）C and pET32a. After expressing in E.coli BL21, the truncated proteins of Dxl6 RS domain part and Dxl6 middle part in pGEX-4T-1His（6）C were purified and used to immunize rabbits. Purified antibodies against the RS domain and Dxl6 middle part were obtained by affinity chromatography with the expressed products of Dxl6 RS domain part and Dxl6 middle part in pET32a, respectively. The result shows the antibody against Dxl6 RS domain has a good specificity to Dxl6 in Drosophila larvae by Western blot analysis.     

大负子蝽卵壳结构和雄虫孵卵生物学的研究

负子蝽Diplonychus, major Esaki因雌虫产卵于雄虫背部,雄虫负卵孵化而得名。笔者对大负子蝽卵粒的表面结构、卵粒发育环境要求和负卵雄虫行为进行了研究,认为负子蝽雄虫负卵孵化的主要意义在于为卵粒提供适宜的水分、氧气条件,保证胚胎正常发育。现将研究结果介绍如下。 一、卵壳结构的观察 卵粒经4％戊二醛固定,丙酮逐级脱水,真空镀膜后于日立S-450型扫描电镜下观察、拍片。 初产卵淡黄色,长1.7—2.5mm,直径1.1—1.5mm,随发育逐渐伸长,近孵化时达3.5—4.0mm,直径基本不变。电镜下依肉纹的差异可将卵壳表面分为三部分:基部,包括A、B区;中部,包括C、D、E区;端部,包括F、G区(图版Ⅰ:1)。     

负子蝽交配、产卵及孵化的观察

<正> 负子蝽Diplonychus sp.能捕食蚊类的幼虫、蛹,但也捕食鱼苗,对养鱼业有一定的为害。我们在室内对该虫进行了饲养观察,现将有关生物学将性介绍如下。 负子蝽其成虫体呈扁平、椭圆形,淡黄褐色。长约21mm,宽约11mm,雌虫稍大于雄虫。成虫虽有翅,但不能飞翔,大部分时间都生活于水中,以捕食小型水生动物为生。该虫交配、产