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Stanley M. Hollenberg Jiunu S. Lai Joachim L. Weickmann Takayasu Date 《Analytical biochemistry》1984,143(2):341-349
Two novel mutagenesis techniques to specifically alter the sequence of plasmid DNA have been developed. In contrast to other primer-directed mutagenesis methods which require a single-stranded, closed-circular template, a linearized single strand was used as the mutagenesis template. The template is prepared by restriction enzyme digestion of covalently-closed-circular plasmid DNA. These methods are simple, require small amounts of plasmid DNA, and can result in a relatively high frequency of mutagenesis. 相似文献
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Schmidt Hoffmann Weickmann v. Berg Heidt Scherz Kuckuck R. Schick Gustaf de Lattin Hilde Pieper Schwanitz Hassebrauk Roegner-Aust Schröck H. -J. Troll Stelzner Hackbarth Grub P. van Veen R. W. Böhme E. Stein 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1942,14(2):47-56
Ohne Zusammenfassung 相似文献
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Freisleben Weickmann Ernst Eckhard Kuhn F. Resende Straub Schmidt E. Stein J. Straub Heberer H. Ernst Hilde Pieper W. J. Schmidt J. Kisser H. -J. Troll R. Schick Eifrig Aust Schröck Stelzner Schröck Schieblich R. Schick Schwanitz Hilde Pieper Gruber Lang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1941,13(4):88-96
Ohne Zusammenfassung 相似文献
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Lang O. Schwarz Propach Hackbarth Beatus R. Schick Weickmann von Rauch Schieblich Ludwigs Weickmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1939,11(12):348-352
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S Huang R C Elliott P S Liu R K Koduri J L Weickmann J H Lee L C Blair P Ghosh-Dastidar R A Bradshaw K M Bryan 《Biochemistry》1987,26(25):8242-8246
Polypeptides synthesized in the cytoplasm of eukaryotes are generally initiated with methionine, but N-terminal methionine is absent from most mature proteins. Many proteins are also N alpha-acetylated. The removal of N-terminal methionine and N alpha-acetylation are catalyzed by two enzymes during translation. The substrate preferences of the methionine aminopeptidase (EC 3.4.11.x) and N alpha-acetyltransferase (EC 2.3.1.x) have been partially inferred from the distribution of amino-terminal residues and/or mutations found for appropriate mature proteins, but with some contradictions. In this study, a synthetic gene corresponding to the mature amino acid sequence of the plant protein thaumatin, expressed in yeast as a nonexported protein, i.e., lacking a signal peptide, has been used to delineate the specificities of these enzymes with respect to the penultimate amino acid. Site-directed mutagenesis, employing synthetic oligonucleotides, was utilized to construct genes encoding each of the 20 amino acids following the initiation methionine codon, and each protein derivative was isolated and characterized with respect to its amino-terminal structure. All four possible N-terminal variants--those with and without methionine and those with and without N alpha-acetylation--were obtained. These results define the specificity of these enzymes in situ and suggest that the nature of the penultimate amino-terminal residue is the major determinant of their selectivity. 相似文献
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G. Weickmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1938,10(4):89-91
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