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1.
Dielectric properties of human red blood cells (RBCs) in suspension (hematocrit 50%) from 243 healthy persons (120 males, 123 females) were measured at 25 °C in a frequency range of 1–500 MHz, with a coaxial transmission line reflection method (one-side measurement). The measuring system, controlled by an IBM-PC computer, was composed of a network analyzer (HP4195A), an impedance test adapter (HP41951-61001), a coaxial line sensor, and a temperature-controlling set. The data measured revealed a statistically significant age dependence, with a critical age of about 49 years, above which permittivity and conductivity of human RBCs in suspension decreased significantly. © 1994 Wiley-Liss, Inc. 相似文献
2.
本实验采用免疫细胞化学方法观察19例人胎视网膜内Parvalbumin(PV)和Calbi-din-D28k(CaBP)免疫反应神经元的分布和发育,对它们在人胎视网膜发育中的演变规律进行了研究。结果显示,PV和CaBP免疫反应神经元属于视网膜水平细胞,无长实细胞和节细胞的不同亚群,CaBP免疫反应神经元还可能分属于视细胞。PV和CaBP免疫反应神经元的发育主要是在胚胎中期,胎14周时它们已分别出现于视网膜内的不同部位,其各自的演变规律不同,至胚胎27周时初步建立了各自的分布模式。在整个发育过程,颞测机网膜内PV免疫反应神经元的密度及反应强度均高于鼻侧视网膜内的PV免疫反应神经元,PV和CaBP免疫反应神经元的发育与视网膜的组织发生也有着密切的联系。本文结果提示,PV和CaBP可能在视网膜神经元的分化、迁移及突触形成中分别起着重要的作用。 相似文献
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本文对准分子激光PRK治疗近视眼息者503例的早期临床结果进行分析,近视范围为-2.0至-15.0D,根据术前屈光度不同分为三组,术后随访三个月.结果证实准分子激光是一种安全、有效、预测性较好的治疗近视眼方法.全部病人提高了裸眼视力,绝大部分保持了最佳矫正视力,且没有产生危及视力的并发症. 相似文献
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用闪光诱导瞬间二向色性方法测量了蔗糖和甘油对菌紫质(BR)分子在脂质泡中的旋转扩散运动的影响。结果表明脂质囊泡本身在悬浮液中的运动并不会影响BR分子在膜中的旋转运动的测量。蔗糖和甘油对BR旋转运动的影响在相变温度以上和相变温度以下是不同的,相变温度以下的主要作用是相分离,使运动减慢,相变温度以上的作用可能是脂的分散,使运动加快。 相似文献
7.
本文报道低温胁迫下风眼莲叶片脱落酸(ABA)、可溶性蛋白质和水势的测定结果。低温胁迫时脱落酸和可溶性蛋白质含量远高于对照,(前者含量最高可达对照的4倍,后者可达到对照的2.75倍),而且脱落酸和蛋白质含量随温度降低而升高。蛋白质的生物合成抑制剂亚胺环己酮证明,可溶性蛋白质含量升高,原因是有部分是新合成的。在各种低温处理下获得了几乎相同于对照的叶片水势。我们推测:低温胁迫下,脱落酸水平的相应变化不是由于低温诱导水分胁迫所致,而是低温胁迫本身诱导。 相似文献
8.
Wensi Fan Ran Zhang Dong Han Zhenhua Jiang Shuang Li Jibin Zhang Yanhua Li Yabin Wang Feng Cao 《Journal of cellular and molecular medicine》2020,24(10):5476-5490
Diabetes mellitus causes endothelial dysfunction, which further exacerbates peripheral arterial disease (PAD). Improving endothelial function via reducing endothelial oxidative stress (OS) may be a promising therapy for diabetic PAD. Activation of liver X receptor (LXR) inhibits excessive OS and provides protective effects on endothelial cells in diabetic individuals. Therefore, we investigated the effects of LXR agonist treatment on diabetic PAD with a focus on modulating endothelial OS. We used a streptozotocin-induced diabetes mouse model combined with a hindlimb ischaemia (HLI) injury to mimic diabetic PAD, which was followed by LXR agonist treatment. In our study, the LXR agonist T0901317 protected against HLI injury in diabetic mice by attenuating endothelial OS and stimulating angiogenesis. However, a deficiency in endothelial Sirtuin1 (SIRT1) largely inhibited the therapeutic effects of T0901317. Furthermore, we found that the underlying therapeutic mechanisms of T0901317 were related to SIRT1 and non-SIRT1 signalling, and the isoform LXRβ was involved in LXR agonist-elicited SIRT1 regulation. In conclusion, LXR agonist treatment protected against HLI injury in diabetic mice via mitigating endothelial OS and stimulating cellular viability and angiogenesis by LXRβ, which elicited both SIRT1-mediated and non-SIRT1-mediated signalling pathways. Therefore, LXR agonist treatment may be a promising therapeutic strategy for diabetic PAD. 相似文献
9.
Yan Li Jiali Li Zhihui Chen Yi Wei Yanhua Qi Changyin Wu 《Plant biotechnology journal》2020,18(10):2015-2026
Rice tiller angle determines plant growth density and further contributes grain production. Although a few genes have been characterized to regulate tiller angle in rice, the molecular mechanism underlying the control of tiller angle via microRNA is poorly understood. Here, we report that rice tiller angle is controlled by OsmiR167a‐targeted auxin response factors OsARF12, OsARF17 and OsARF25. In the overexpression of OsMIR167a plants, the expression of OsARF12, OsARF17 and OsARF25 was severely repressed and displayed larger tiller angle as well as the osarf12/osarf17 and osarf12/ osarf25 plants. In addition, those plants showed compromised abnormal auxin distribution and less sensitive to gravity. We also demonstrate that OsARF12, OsARF17 and OsARF25 function redundantly and might be involved in HSFA2D and LAZY1‐dependent asymmetric auxin distribution pathway to control rice tiller angle. Our results reveal that OsmiR167a represses its targets, OsARF12, OsARF17 and OsARF25, to control rice tiller angle by fine‐tuning auxin asymmetric distribution in shoots. 相似文献
10.
Ming Cui Wenjia Shen Weiwei Qin Xu Wang Yanhua Li Fei Xu Zhenlei Xin 《Journal of biochemical and molecular toxicology》2020,34(5)
Atherosclerosis is one of the most common and crucial heart diseases involving the heart and brain. At present, atherosclerosis and its major complications comprise the leading causes of death worldwide. Our purpose was to identify the role of ciRS‐7 in atherosclerosis. Tubulogenesis of HMEC‐1 cell was evaluated utilizing tube formation assay. Cell Counting Kit‐8 assay and flow cytometry were utilized to test viability and apoptosis. Migration assay was utilized to determine the migration capacity of experimental cells. Western blot was applied to examine apoptosis and tube formation‐associated protein expression. In addition, the above experiments were repeated when silencing ciRS‐7, overexpressing ciRS‐7, and upregulating miR‐26a‐5p. HMEC‐1 cells formed tube‐like structures over time. Silencing ciRS‐7 suppressed viability, migration, and tube formation but promoted apoptosis. Oppositely, overexpressing ciRS‐7 reversed the effect in HMEC‐1 cells. miR‐26a‐5p expression was elevated by silencing ciRS‐7 and reduced by overexpressing ciRS‐7. Moreover, overexpressing ciRS‐7 facilitated viability, migration, and tube formation via upregulating miR‐26a‐5p. Conclusively, overexpressing ciRS‐7 mobilized phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) pathway and suppressed c‐Jun N‐terminal kinase (JNK)/p38 pathway. ciRS‐7 exerted influence on apoptosis, viability, migration, and tube formation through mediating PI3K/AKT and JNK/p38 pathways by miR‐26a‐5p downregulation in HMEC‐1 cells. 相似文献