排序方式: 共有67条查询结果,搜索用时 22 毫秒
1.
2.
Tsukushi S Kajita M Nakamura S Niwa T 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2002,776(1):133-137
To determine if the erythrocyte levels of 3-deoxyglucosone (3-DG) are increased in diabetic patients, and if they correlate with glycemic status, they were measured in diabetic patients without renal disease as well as in healthy subjects. The erythrocyte levels of 3-DG were measured by a selected ion monitoring method of gas chromatography-chemical ionization mass spectrometry using [(13)C(6)]-3-DG as an internal standard. The erythrocyte levels of 3-DG were significantly higher in diabetic patients than in healthy subjects. The erythrocyte concentration of 3-DG was significantly and positively correlated with HbA1c (r=0.84, P<0.001). However, no significant correlation could be found between erythrocyte 3-DG and age, onset age of diabetes, or duration of diabetes in our group of diabetic patients. In diabetes, the production of 3-DG in the erythrocytes is increased via the polyol pathway and/or the Maillard reaction due to hyperglycemia. 相似文献
3.
The impact of masticatory ability as evaluated by salivary flow rates on obesity in japanese: The Toon health study 下载免费PDF全文
4.
NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers 总被引:6,自引:0,他引:6
Okubo S Hara F Tsuchida Y Shimotakahara S Suzuki S Hatanaka H Yokoyama S Tanaka H Yasuda H Shindo H 《The Journal of biological chemistry》2004,279(30):31455-31461
A member of the PIAS (protein inhibitor of activated STAT) family of proteins, PIAS1, have been reported to serve as an E3-type SUMO ligase for tumor suppressor p53 and its own. It also was proposed that the N-terminal domain of PIAS1 interacts with DNA as well as p53. Extensive biochemical studies have been devoted recently to understand sumoylations and its biological implications, whereas the structural aspects of the PIAS family and the mechanism of its interactions with various factors are less well known to date. In this study, the three-dimensional structure of the N-terminal domain (residues 1-65) of SUMO ligase PIAS1 was determined by NMR spectroscopy. The structure revealed a unique four-helix bundle with a topology of an up-down-extended loop-down-up, a part of which the helix-extended loop-helix represented the SAP (SAF-A/B, Acinus, and PIAS) motif. Thus, this N-terminal domain may be referred to as a four-helix SAP domain. The glutathione S-transferase pull-down assay demonstrated that this domain possesses a binding ability to tumor suppressor p53, a target protein for sumoylation by PIAS1, whereas gel mobility assays showed that it has a strong affinity toward A/T-rich DNA. An NMR analysis of the four-helix SAP domain complexed with the 16-bp-long DNA demonstrated that one end of the four-helix bundle is the binding site and may fit into the minor groove of DNA. The three-dimensional structure and its binding duality are discussed in conjunction with the biological functions of PIAS1 as a SUMO ligase. 相似文献
5.
Narita J Ishida S Okano K Kimura S Fukuda H Kondo A 《Applied microbiology and biotechnology》2006,73(2):366-373
The 5′-untranslated leader sequence (UTLS) of the slpA gene from Lactobacillus acidophilus contributes to mRNA stabilization by producing a 5′ stem and loop structure, and a high-level expression system for the lactic acid bacteria was developed using the UTLS in this study. A plasmid, which expresses α-amylase under the control of the ldh promoter, was constructed by integrating the core promoter sequence with the UTLS. The role of the UTLS in increasing the copies of the α-amylase mRNA was proved by measuring α-amylase activity in the culture supernatant and the relative expression of α-amylase mRNA was determined by the quantitative real-time PCR analysis. Moreover, several expression systems were constructed by combining the core promoter sequence with the UTLS or with the partially deleted UTLS and the expression level was evaluated. The use of the UTLS led to the success in improving α-amylase expression in the two strains of Lactobacillus casei and Lactococcus lactis. The current study showed that the improvement in protein production using the UTLS could be applied to the expression system in the lactic acid bacteria. 相似文献
6.
Gaina Alexandru Zhang Miao Sekine Michikazu Hamanishi Shimako Wang Hongbin Nasermoadeli A. Kagamimori Sadanobu 《Sleep and biological rhythms》2004,2(2):144-149
Sleep and Biological Rhythms - The sleep-promoting effect of a hot bath on daytime sleep after night-shift work was assessed by polysomnography. Having an unlimited daytime sleep opportunity,... 相似文献
7.
Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza 总被引:8,自引:0,他引:8
Sakurako Uozu Hiroshi Ikehashi Nobuko Ohmido Hisako Ohtsubo Eiichi Ohtsubo Kiichi Fukui 《Plant molecular biology》1997,35(6):791-799
Large variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosomal length (r_0.939) and the total chromosomal area (r_0.927) correlated well with the nuclear DNA content. Among all the species examined, Oryza australiensis (E genome) and O. brachyantha (F genome), respectively, were the largest and smallest in genome size. O. sativa (A genome) involving all the cultivated species showed the intermediate genome size between them. The distribution patterns of genome-specific repetitive DNA sequences were physically determined using fluorescence in situ hybridization (FISH). O. brachyantha had limited sites of the repetitive DNA sequences specific to the F genome. O. australiensis showed overall amplification of genome-specific DNA sequences throughout the chromosomes. The amplification of the repetitive DNA sequences causes the variation in the chromosome morphology and thus the genome size among diploid species in the genus Oryza. 相似文献
8.
Junko Amemura-Maekawa Sakurako Mishima-Abe Fumiaki Kura Tomoko Takahashi Haruo Watanabe 《FEMS microbiology letters》1999,176(2):339-344
A gene katA that encodes a novel catalase-peroxidase was cloned from the chromosome of Legionella pneumophila. The nucleotide sequence revealed that KatA was highly homologous to members of the bacterial bifunctional catalase-peroxidase family. In addition, KatA has a N-terminal signal sequence and was considered to be present in the periplasm of the bacterium. 相似文献
9.
10.
Hirokazu Uemura Sakurako Katsuura-Kamano Miwa Yamaguchi Fusakazu Sawachika Kokichi Arisawa 《PloS one》2014,9(4)