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Hu Yan-Yu Wei Hai-Wei Zhang Zhi-Wei Hou Shuang-Li Yang Jun-Jie Wang Jun-Feng Lü Xiao-Tao 《Plant and Soil》2020,453(1-2):503-513
Plant and Soil - Uncovering the importance of soil and plant characteristics in driving the legacy effects of nitrogen (N) deposition on plant community nutrient stoichiometry would improve our... 相似文献
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Zhen-Wei Wu Hai-Wei Xu Gui-Fu Dai Meng-Jiao Liu Li-Ping Zhu Jian Wu Ya-Nan Wang Feng-Juan Wu Dan Zhao Ming-Fu Gao Shan-Shan Nie Wei Han Jing-Hui Song Hong-Min Liu 《Bioorganic & medicinal chemistry letters》2013,23(23):6421-6426
In the present study, andrographolide (Andro, 1) derivatives were screened to identify potent inhibitors against tumor-cell migration and invasion, and associated structure–activity relationships were studied. Compared to 1, compounds 8a–8d exhibited more potent activities against migration in SGC-7901, PC-3, A549, HT-29 and Ec109 cell lines. Improved activities against tumor-cell migration and invasion were proved to be associated with the down-regulation of MMPs. 相似文献
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目的:观察大鼠长期皮下注射叠氮钠后的学习记忆、中枢神经系统β-淀粉样蛋白含量的改变。方法:采用Morris水迷宫、放射免疫和透射电镜的方法。结果:大鼠出现明显的空间记忆功能障碍,海马和额叶大脑皮层内Aβ含量升高。结论:长期皮下注射叠氮钠可以制备AD模型,并导致中枢Aβ的升高。 相似文献
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Andrographolide (1), the cytotoxic agent of the plant Andrographis paniculata, was subjected to semi-synthetic studies leading to a series of new derivatives, a novel family of glucosidase inhibitors. Nicotination of 3,19-hydroxyls in 15-alkylidene andrographolide derivatives (9) was favorable to alpha-glucosidase inhibition activity. Among them, 15-p-chlorobenzylidene-14-deoxy-11,12-didehydro-3,19-dinicotinateandrographolide (11c) was a very potent inhibitor against alpha-glucosidase with an IC50 value of 6 microM. However, all compounds concerned for beta-glucosidase showed no inhibition. All compounds synthesized were characterized by the analysis of NMR, IR, HRMS spectra and the stereochemistry of 2 was confirmed by X-ray analysis. 相似文献
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Identification of immunodominant Th1-type T cell epitopes from Schistosoma japonicum 28 kDa glutathione-S-transferase, a vaccine candidate 总被引:1,自引:0,他引:1
Li GF Wang Y Zhang ZS Wang XJ Ji MJ Zhu X Liu F Cai XP Wu HW Wu GL 《Acta biochimica et biophysica Sinica》2005,37(11):751-758
Th1-type cytokines produced by the stimulation of Th 1-type epitopes derived from defined schistosome-associated antigens are correlated with the development of resistance to the parasite infection. Schistosoma mansoni 28 kDa glutathione-S-transferase (Sm28GST), a major detoxification enzyme, has been recognized as a vaccine candidate and a phase II clinical trial has been carried out. Sheep immunized with recombinant Schistosoma japonicum 28GST (Sj28GST) have shown immune protection against the parasite infection. In the present study, six candidate peptides (P1, P2, P3, P4, P7 and P8) from Sj28GST were predicted, using software, to be T cell epitopes, and peptides P5 and P6 were designed by extending five amino acids at the N-terminal and C-terminal of P1, respectively. The peptide 190-211 aa in Sj28GST corresponding to the Th1-type epitope (190-211 aa) identified from Sm28GST was selected and named P9. The nine candidate peptides were synthesized or produced as the fusion protein with thioredoxin in the pET32c(+)/BL21(DE3) system. Their capacity to induce a Th1-type response in vitro was measured using lymphocyte proliferation, cytokine detection experiments and flow cytometry. The results showed that P6 (73-86 aa) generated the strongest stimulation effect on T cells among the nine candidate peptides, and drove the highest level of IFN-γ, and IL-2. Therefore, P6 is a functional Thl-type T cell epitope that is different from that in Sm28GST, and will be useful for the development of effective vaccines which can trigger acquired immunity against S. japonicum. Moreover, our strategy of identifying the Thl-type epitope by a combination of software prediction and experimental confirmation provides a convenient and cost-saving alternative approach to previous methods. 相似文献
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不同食料植物对美国白蛾生长发育和繁殖的影响 总被引:1,自引:0,他引:1
2010年6月至7月,在室内研究了美国白蛾Hyphantria cunea(Drury)第1代幼虫对洋白蜡、欧美107号杨、法桐和白榆的取食量及不同的食料种类对美国白蛾生长发育和繁殖的影响。结果表明,美国白蛾对受试的各树种的取食量以洋白蜡为最多,完成一代平均每头可以取食2.21 g,其次为欧美107号杨1.95 g,再次为法桐和白榆,分别为1.85 g/头和1.68 g/头。3龄后幼虫对不同的寄主呈现出一定的偏好性,特别是5龄和6龄幼虫对不同的寄主的取食量表现出显著差异。不同的食料植物对美国白蛾幼虫和蛹的发育历期、存活率、蛹重、成虫寿命、产卵量等有显著影响。取食法桐对其生长发育和繁殖表现出明显的不利性,主要表现为幼虫期延长、蛹重减轻、单雌产卵量降低。 相似文献
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Hai-Wei Fan Xue-Chao Zhang Yi-Peng Xu Xiao-Wen Cheng Chuan-Xi Zhang 《Journal of virology》2012,86(21):11941
Bombyx mori nucleopolyhedrovirus (BmNPV), a member of the Baculoviridae, is a major pathogen of silkworm and has also been recently developed as an expression vector for heterologous gene expression in the silkworm larvae and pupae. To better understand the diversity of this important baculovirus, we sequenced the complete genome of the BmNPV strain isolated from India, where its host is available throughout the year due to its tropical climate. The genome of the Indian strain consists of 127,879 nucleotides, with a G+C content of 40.36%. There are 138 open reading frames (ORFs) encoding the predicted proteins of more than 50 amino acids. Genomic comparison of the Indian strain with 3 other reported BmNPV strains showed that the baculovirus repeat ORFs (bro) and homologous repeat regions (hr''s) are highly variable. These results suggest that the BmNPV strain heterogeneity is mainly caused by single-nucleotide polymorphisms (SNPs) and changes in the hr''s and bro genes. 相似文献