甜菜碱醛脱氢酶在菠菜叶细胞中的定位

将菠菜叶片匀浆后．用差速离心和梯度率心分离叶绿体、过氧物酶体、微粒体等细胞器和１０００００×ｇ上清法部分。用酶活测定法测定各部分甜菜碱醛脱氢酶（ＢＡＤＨ）的活性;用免疫扩散法鉴定各组分的ＢＡＤＨ。除叶绿体外,过氧物酶体、微粒体．以及１０００００×ｇ上清液中也存在ＢＡＤＨ。     

干旱和盐胁迫诱导甜菜叶中的甜菜碱醛脱氢酶的积累

应用双向免疫扩散方法测定表明,甜菜叶片的甜菜碱醛脱氢酶能与菠菜的甜菜碱醛脱氢酶抗体发生交叉反应。渗透势－０．６５￣－２．６ＭＰａ的甘露醇溶液或２００～３００ｍｍｏｌ／Ｌ的ＮａＣｌ溶液,诱导甜茶叶片甜菜碱醛脱氢酶积累明显增加。     

Indication of dynamic neurovascular coupling from inconsistency between EEG and fMRI indices across sleep–wake states

Sleep and Biological Rhythms - Neurovascular coupling (NVC), the transient regional hyperemia following the evoked neuronal responses, is the basis of blood oxygenation level-dependent techniques...     

来源于麦氏喙枝孢霉的玉米赤霉烯酮水解酶在毕赤酵母中的高效表达及活性分析

【背景】玉米赤霉烯酮(zearalenone,ZEN)及其衍生物是一群具有雌激素活性的霉菌毒素,广泛存在于被霉菌污染的谷物中,造成食品业和畜牧业的巨大损失。利用专一性高的水解酶进行生物转化可有效去除玉米赤霉烯酮。【目的】构建高效表达玉米赤霉烯酮水解酶的酵母系统,以促进玉米赤霉烯酮水解酶的研究和工业应用。【方法】将来源于麦氏喙枝孢霉(RhinocladiellamackenzieiCBS650.93)的Rmzhd基因转入毕赤酵母中,筛选获得高效表达菌株,通过高效液相色谱分析发酵液中重组酶的性质。【结果】发酵液中Rm ZHD对ZEN的酶活力为16.67 U/m L,对α-ZOL的酶活力为9.85 U/m L。SDS-PAGE检测表达产物的分子量,与理论值30.7k D符合,且发酵上清液蛋白纯度高。Rm ZHD的最适p H值为9.6,最适温度为45°C,并具有较好的耐热性。【结论】研究结果为玉米赤霉烯酮水解酶的异源表达及其潜在的工业应用提供了一定的指导。     

A rice phenomics study—phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population

With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene function. As an important crop and a model organism, rice provides major insights into gene functions important for crop growth or production. Phenomics with detailed information about tagged populations provides a good tool for functional genomics analysis. By a T-DNA insertional mutagenesis approach, we have generated a rice mutant population containing 55,000 promoter trap and gene activation or knockout lines. Approximately 20,000 of these lines have known integration sites. The T0 and T1 plants were grown in net “houses” for two cropping seasons each year since 2003, with the mutant phenotypes recorded. Detailed data describing growth and development of these plants, in 11 categories and 65 subcategories, over the entire four-month growing season are available in a searchable database, along with the genetic segregation information and flanking sequence data. With the detailed data from more than 20,000 T1 lines and 12 plants per line, we estimated the mutation rates of the T1 population, as well the frequency of the dominant T0 mutants. The correlations among different mutation phenotypes are also calculated. Together, the information about mutant lines, their integration sites, and the phenotypes make this collection, the Taiwan Rice Insertion Mutants (TRIM), a good resource for rice phenomics study. Ten T2 seeds per line can be distributed to researchers upon request. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. Chyr-Guan Chern, Ming-Jen Fan, and Su-May Yu have contributed equally to this work.     

增温对高寒草甸群落凋落物质量动态变化的影响

为了解未来增温条件下,青藏高原高寒草甸凋落物质量如何变化,将有助于增强对高寒草甸生态系统碳源/汇效应的认识.该文通过定位可控的增温试验平台,动态监测了凋落物生物量及其质量的变化.结果表明:增温显著地促进了凋落物的分解速率(F=35.757,P＜0.001),降低了凋落物中的C、N含量及其C/N比,但提高了凋落物中的P含...     

Proteomics-based identification of autoantibodies in the sera of healthy Chinese individuals from Beijing

The identification of panels of tumor antigens that elicit an antibody response may have utility in cancer screening, diagnosis and in establishing prognosis. However, autoantibodies normally exist in sera of healthy individuals and are enormously diversified. To explore the reservoir of autoantibody in healthy population, we performed a proteomics investigation of autoantibody profiles in the sera of 36 healthy Chinese individuals from Beijing, which may provide valuable reference information to the identification of disease-specific autoantibodies. The results showed that autoantibody profiles varied individually, but some autoantibodies were identified at a high frequency in the healthy population. The autoantibodies against alpha-enolase and those against heterogeneous nuclear ribonucleoprotein L were positive in more than 50% of the sera samples. The autoantibodies identified in more than 20% of samples included those against annexin II, F-actin capping protein beta subunit and calreticulin. Some of these autoantibodies have been previously reported to be involved in autoimmune conditions and cancers. Autoantibodies in the healthy population are important as a foundation from which disease-specific autoantibodies can be defined. Thus our report on autoantibodies in healthy individuals may be useful as a reference for defining new autoantibody biomarkers.     

中国蓼属春蓼组植物果实形态及果皮微形态的研究

应用解剖镜和扫描电镜对中国产蓼属春蓼组（Polygonum sect. Persicaria）19种3变种植物的果实形态和果皮微形态特征进行了观察和研究。结果表明,蓼属春蓼组植物的果实形态为卵形或椭圆状卵形,具三棱、双凹或双凸,顶端渐狭,有喙或无喙;果皮微形态可分为7种类型： 洼点、浅洼点,脑纹状纹饰,拟脑纹状纹饰,网状纹饰,不规则褶皱,不规则小疣状颗粒以及密浅网状纹饰。观察结果支持将绵毛酸模叶蓼（P. lapathifolium L. var. salicifolium Sibth.）合并到酸模叶蓼（P. lapathifolium L.）,做为酸模叶蓼的异名处理;支持将长鬃蓼（P. longisetum De Br.）作为丛枝蓼（P. posumbu Buch. Ham. ex D.Don）的变种处理的意见;认为密毛酸模叶蓼（P. lapathifolium L. var. lanatum (Roxb.) Stew.）应恢复种级,圆基长鬃蓼（P. longisetum De Br. var. rotunatum A.J.Li）应升为种级;支持平武蓼（Polygonum pingwuense F. Z. Li et Y. T. Hou et S. J. Fan, sp. nov.）新种的成立。     

非侵入性基因治疗在中枢神经系统疾病中的应用

非侵入性地将具有治疗作用的基因通过血脑屏障输送至脑内,以治疗中枢神经系统疾病,是生物学领域研究的难点和热点,而应用适宜的转运载体是解决这一难题的有效途径。使用病毒载体或非病毒载体,已成功进行非侵入性基因治疗的中枢神经系统疾病有实验性运动神经疾病、脑部肿瘤和帕金森病等。随着对脑微血管内皮细胞上的受体的研究和新型载体的开发,应用非侵入性基因治疗中枢神经系统疾病将会更为广泛。