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家蝇cDNA文库的构建 总被引:10,自引:0,他引:10
自Boman小组 (Steiner ,1981)从惜古比天蚕(Hyalophoracecropia)中分离、纯化出第一种抗菌肽天蚕素以来 ,人们已在昆虫和其他无脊椎动物中发现了 170多种抗菌肽 (Lowenbergeretal ,1999)。目前 ,人们不仅搞清楚了多数抗菌肽的氨基酸序列、结构和功能特点 ,而且还对一些抗菌肽的基因进行了克隆 (陈留存和王金星 ,1999)。我们以家蝇为材料 ,分离、纯化出了抗菌肽 ,在此基础上 ,构建了经过诱导的家蝇cDNA文库 ,以克隆其基因。1 材料和方法1 1 实验材料家蝇 (Muscadomestica)… 相似文献
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家蝇防御素基因的cDNA克隆及序列分析 总被引:12,自引:0,他引:12
Defensin is a kind of cationic.inducible antimicrobial peptide found in a large range of living organisms that contributes to host defense by disrupting the cytoplasmic membrane of microorganisms.with their broad antimicrobial spectrum and strong pharmaceutical effects.antimicrobial peptides,including defensins,represent a source of novel antibiotic agents.A novel full-length 430 base pairs cDNA of an insect defensin was cloned using polymerase chain reaction (PCR) from the cDnA library of houseflies(Musca domestica) that had been challenged by E.coli and staphylococcus taincd an NH2-terminal signal sequence(1-22)followed by a propeptide and the mature peptide(53-92),The sequence identity with other insect defensin is between 51% and 73%.The mature peptide,with a predicted molecular weight of 4.0kDa,and pI of 8.69,has 1 negative charged amino acid and 4 positice ones,the putative housefly defensin is characterized by 6 invariant cysteine residues forming 3 disulfide bonds,Cys1-Cys4,Cys2-Cys5 and Cys3-Cys6,These results suggest that the novel full-length cDNA of the defensin gene.Denominated Mdde,has been successfully cloned from houseflies. 相似文献
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南四湖泥鳅和大鳞副泥鳅随机扩增多态DNA分析初报 总被引:1,自引:0,他引:1
采用随机扩增多态性DNA(RAPD)技术对南四湖的泥鳅(Misgurnus anguillicaudatus)和大鳞副泥鳅(Paramisgurnus dabryanus)进行了初步分析。共用10个10bp的OPG组寡核苷酸片段作为引物,筛选出结果好的5个引物,从泥鳅4个体基因组DNA中扩增出142个片段,共有片段12个,特有片段5个;从大鳞副泥鳅3个体基因组DNA中扩增出101个片段,共有片段18个,特有片段5个,在总扩增出的243个片段中,共有片段仅4个。所有扩增片段大小介于0.2-3kb之间。用PoPGen32软件计算出个体间的遗传距离,依此进行UPGMA聚类得到的分子系统树与形态分类学的结果一致,这7个体被聚成2大类,正对应着形态学的2个种:泥鳅和大鳞副泥鳅。 相似文献
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