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本研究利用胚胎整体原位杂交技术结合系列组织学切片技术,以及Northern blot 分析,对进化上处于特殊地位的模式动物文昌鱼profilin 基因不同发育时期的表达图式进行了系统研究,揭示了该基因的动态表达图式与肌肉分化的过程一致,文昌鱼profilin 基因可能与胚胎早期肌肉的发育相关。同时,通过Southern blot 检测对青岛文昌鱼profilin 基因可能存在的同源体进行了探讨,试图为弄清文昌鱼肌肉发育分化的分子机制提供资料。 相似文献
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当今,在世界上各科学技术发达的国家内,不论是政府、科技界或一般人士均关注着“生物工程”的发展及其可能为人类谋取的巨大福利。这是因为目前生物科学和工程技术的发展,已使它们之间能在非常精密而有效的基础上结合在一起,为人类创造新的物质财富。在过去的年代里,人类社会借助于科学技术的发展,把它们的成果转化为生产力,成为人类创造生活资料和其它财富的源泉。因此把科学研究中基 相似文献
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Maturation process of zebrafish oocyte was investigated using in vitro incubation.In medium EM-199 containing 0.5 μg/ml of 17α-hydroxyprogesterone incubated under 80% O_2 and at 25°C,germinal vesicles(GV)of oocytes in stage Ⅳ migrated from midway between the center and theperiphery ofoocytes to the periphery in 40 minutes and the oocytes went into stage V.Half an hourlater,the oocytes underwent germinel vesicle breakdown(GVBD)with a breakdown rate of 59%.Two more hours were needed for such oocytes to complete their final maturation.The mature eggscould not come off from the follicle layer surrounding them by themselves(ovulation).By removingthe follicle and adding active sperms for insemination,we could make the mature eggs fertilized.Thechorion was elevated and blastoderm formed on the animal pole.The cleavage and development ofthese fertilized eggs followed the same course as the naturally matured and fertilized eggs.Usingblastula formation as a marker of successful fertilization of the in vitro matured egg,the fertilizationrate was 78%.This is the first report on the successful in vitro incubation of mature oocytes inzebrafish.The establishment of this in vitro oocyte maturation technology has laid the foundationfor further investigation of the transfer of foreign genes in the germinal vesicles of oocytes. 相似文献
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Ⅰ引言 在每次受精过程中均存在着将基因从一个有机体转移到另一个有机体的情况,其结果便产生了一些遗传物质的新的特殊组合。 相似文献
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