首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1篇
  完全免费   2篇
  2017年   1篇
  2005年   1篇
  2001年   1篇
排序方式: 共有3条查询结果,搜索用时 31 毫秒
1
1.
秸秆覆盖免耕土壤真菌群落结构与生态特征研究   总被引:19,自引:1,他引:18       下载免费PDF全文
高云超  朱文珊  陈文新 《生态学报》2001,21(10):1704-1710
不同耕作方法土壤真菌群落结构和生态特征分析表明,翻耕0-10cm土层土壤真菌群落含有29种真菌,其中以Chrysosporium merdarium为优势种;翻耕10-20cm土层含有17种真菌,以Sterile black A为优势种:翻耕20-30cm土层含有10种真菌,其中以Chaetomium bostrychodes为优势种,铁茬0-10cm含16种真菌,其中Sterile black A是优势种;铁茬10-20cm土层含有26种真菌,优势种为Sterile black A;铁茬20-30cm含有14种真菌,其中Chaetomium bostrychodes为优势种,免耕0-10cm土层由23种真菌构成,Trichoderma viride和T.koningii为优势种,免耕10-20cm土层由14个种类构成,Talaromyces trachyspermus为优势种,免耕20-30cm土壤由9种真菌组成,其中Talaromyces trachypermus为优势种,免耕土壤真菌群落的多样性和均匀度指数均较高,主成分分析表明,土壤耕作形成了特征性的真菌区系。  相似文献
2.
Soil fungal communities were studied using 18S rDNA-based molecular techniques. Soil DNA was analyzed using temperature gradient gel electrophoresis (TGGE), single-stranded conformational polymorphism (SSCP), cloning and sequencing methods, following community DNA extraction and polymerase chain reaction (PCR). The extracted community DNA was successfully amplified using the primer pair of EF4f-Fung5r which produced ca. 550bp 18S rDNA fragments. TGGE screening of the PCR products showed some differences in band position and intensity between two soil samples in adjacent natural forest (YNF) and hoop pine plantation (YHP) ecosystems at Yarraman in subtropical Australia. TGGE and SSCP could be used for screening PCR products. However, care must be exercised when interpreting the TGGE and SSCP results with respect to microbial diversity, because one band may not necessarily represent one species. It is recommended that the PCR products should be purified before TGGE or SSCP screening. SSCP screening of the clone sequences revealed differences among the clones. Sequence and phylogenetic analyses revealed that all obtained clones were affiliated to the kingdom Fungi, including three phyla, i.e., Zygomycota, Ascomycota and Basidiomycota. Our results suggested that community DNA extraction, PCR, cloning, SSCP screening of clones, sequencing of selected clones and phylogentic analyses could be a good strategy in investigation of soil fungal community and diversity.  相似文献
3.
[目的]为了解不同枸杞产区枸杞根际真菌多样性的差异,从枸杞根际土壤真菌群落的角度试图解释宁夏枸杞优良品质与其土壤微生物菌群的联系。[方法]采用MiSeq高通量测序方法,分别将我国4个不同地区枸杞根际土壤样品进行ITS区的高通量测序,并分析了物种组成和丰度、Alpha多样性、Beta多样性和菌群结构,测定不同产区枸杞主要有效成分,同时对枸杞品质和其土壤理化因子及枸杞根际真菌种群多样性的相关性做了分析。[结果]多糖含量和甜菜碱含量均是中宁枸杞高于兴仁和精河,格尔木含量最低;宁夏兴仁、宁夏中宁、新疆精河3个地区菌群结构类似,子囊菌门和结合菌门占总菌群的80%左右,而青海格尔木除了子囊菌门占58%外,壶菌门和新丽鞭毛菌门远远高于其他3个地区,比例占总菌群的近25%;4个地区枸杞根际土壤真菌菌群结构相似性依次为宁夏兴仁、新疆精河、宁夏中宁、青海格尔木。[结论]枸杞根际土壤样品真菌物种组成丰富,不同地区枸杞根际土壤真菌种群结构有一定差异;枸杞主要有效成分含量与枸杞根际土壤真菌种群结构具有一定相关性。  相似文献
1
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号