Association between ABCB1 gene polymorphisms and fentanyl's adverse effects in Turkish patients undergoing spinal anesthesia

The ATP-binding cassette transporter (ABCB1) gene product, P-glycoprotein plays an important role in the prevention of intracellular accumulation of potentially toxic substances and metabolites in various tissues. Single nucleotide polymorphisms in this gene are claimed to be correlated with changes in the function of P-glycoprotein. There is evidence that fentanyl, may be a substrate for P-glycoprotein. The aim of the study was to assess whether an association exists between ABCB1 gene polymorphism and early respiratory and sedative adverse effects of intravenous fentanyl in Turkish patients who underwent spinal anesthesiaIn all 83 unrelated Turkish patients were enrolled in this study. In this study, spinal anesthesia was provided and a single dose of intravenous fentanyl (2.5 μg.kg⁻¹) at the beginning of surgery was used as a sedative agent. Bispectral index, respiration rate and peripheral oxygen saturation were measured continuously and recorded throughout the study.The allele and genotype frequencies were similar to previous data from Turkish population.Respiratory rate (RR) and SpO₂ parameters of the patients did not show any significant difference according to the genotype distribution for C1236T and C3435T SNPs. Fentanyl-induced decrease in respiration rate was most remarkable at 15 min (23%) in CC genotype of C1236T, whereas in TT genotype of C3435T (18%) polymorphism. SpO₂ parameters in allele distribution were also not significant among the groups (p = 0.374, p = 0.985, respectively). For the C1236T polymorphism, patients carrying T allele showed a significant decrease in pH, and a significant increase in pCO₂ (p < 0.001).ABCB1 polymorphisms did not seem to have a significant effect on sedation and respiratory depression caused by intravenous fentanyl in spinal anesthesia in Turkish patients.     

Stevioside does not cause increased basal insulin secretion or beta-cell desensitization as does the sulphonylurea, glibenclamide: studies in vitro

We have shown that stevioside (SVS) enhances insulin secretion and thus may have a potential role as antihyperglycemic agent in the treatment of type 2 diabetes mellitus. However, whether SVS stimulates basal insulin secretion (BIS) and/or cause desensitization of beta cells like sulphonylureas (SU), e.g. glibenclamide (GB), is not known. To explore and compare the effects of SVS pretreatment with those of GB and glucagon-like peptide-1 (GLP-1), we exposed isolated mouse islets to low or high glucose for 1 h after short-term (2 h) or long-term (24 h) pretreatment with SVS, GB or GLP-1, respectively. BIS at 3.3 or 5.5 mM glucose were not changed after short-term pretreatment with SVS (10(-7) M), while it increased about three folds after pretreatment with GB (10(-7) M). Glucose stimulated insulin secretion (GSIS) (16.7 mM) increased dose-dependently after long-term pretreatment with SVS at concentrations from 10(-7) to 10(-5) M. Pretreatment for 24 h with GB (10(-7) M) increased the subsequent BIS (3.3 mM glucose) (p < 0.001), but decreased GSIS (16.7 mM glucose) (p < 0.001). In contrast SVS (10(-7) M) and GLP-1 (10(-7) M) did not stimulate BIS but both enhanced the subsequent GSIS (16.7 mM glucose) (p < 0.05 and p < 0.05, respectively). While SVS pretreatment increased the intracellular insulin content, GB pretreatment decreased the insulin content. Our study suggests that SVS pretreatment does not cause a stimulation of BIS and does not desensitize beta-cells, i.e. SVS seems to have advantageous characteristics to GB as a potential treatment of type 2 diabetes.     

BIS监测下丙泊酚靶控静脉麻醉与七氟醚麻醉效果比较

目的:比较BIS监测下丙泊酚靶控输注全凭静脉麻醉与七氟醚静吸复合麻醉两种方式下的腹腔镜下子宫切除手术患者麻醉效果。方法:46例择期腹腔镜下子宫切除患者,ASAI`II级,随机分为:丙泊酚靶控全凭静脉麻醉组(TCI组)和七氟醚静吸复合麻醉组(SD组),各23例。术中均监测BIS值(维持在40~60)。记录麻醉诱导前(T1)、手术开始30 min(T2)、术后1 h(T3)患者血压(BP)和心率(HR);记录术后自主呼吸恢复时间、睁眼时间、拔管时间;记录术中知晓发生率和术后24小时恶心呕吐(PONV)发生率。结果:TCI组与SD组患者BP和HR在T1、T2、T3各时间点比较差异均无统计学意义(P0.05);TCI组呼吸恢复时间、睁眼时间、拔管时间均早于SD组,差异有统计学意义(P0.05);两组患者均未发生术中知晓;但术后24小时PONV发生率TCI组明显低于SD组(P0.05)。结论:在BIS监测下,丙泊酚靶控全凭静脉麻醉与七氟醚静吸复合麻醉用于腔镜下子宫切除手术均能够维持术中患者血流动力学平稳,预防术中知晓,但全凭静脉麻醉较静吸复合麻醉患者术后恢复更快,术后24小时恶心呕吐发生率低。     

麻醉诱导前给予依达拉奉与地塞米松联合BIS监测对胸腔镜下肺叶切除术后肺保护与术后恢复的影响

摘要 目的：探讨麻醉诱导前给予依达拉奉与地塞米松联合BIS监测对胸腔镜下肺叶切除术后肺保护与术后恢复的影响。方法：选择2021年1月至2022年8月来我院行胸腔镜下肺叶切除术的患者80例。麻醉诱导前,A组静脉滴注100 mL 0.9 %生理盐水,B组静脉滴注30 mg依达拉奉,C组静脉滴注4 mg地塞米松注射液,D组静脉滴注30 mg依达拉奉及4 mg地塞米松,并对比四组患者相关指标。结果：四组患者的手术时间、麻醉时间、液体量及失血量对比无差异（P>0.05）。四组患者的心率、平均动脉压在T1-T4点时对比无差异（P>0.05）;四组患者的氧合指数、呼吸指数在T1、T2、T4点时对比无差异（P>0.05）;D组氧合指数较A、B、C组高,呼吸指数较A、B、C组低,B组与C组对比无差异（P>0.05）,但氧合指数高于A组,呼吸指数低于A组（P<0.05）。T1、T2、T4点时,四组患者的血清IL-8、IL-10、TNF-α、SP-A水平及多形中性粒细胞数量对比无差异（P>0.05）;D组的SP-A水平较A、B、C组高,IL-8、IL-10、TNF-α及多形中性粒细胞数量较A、B、C组低,B组与C组对比无差异（P>0.05）,但SP-A水平高于A组,IL-8、IL-10、TNF-α及多形中性粒细胞数量低于A组（P<0.05）。D组的术后住院时间较A、B、C组低,B组与C组对比无差异（P>0.05）,但术后住院时间低于A组（P<0.05）。四组的肺炎、肺不张发生率对比无差异（P>0.05）。结论：麻醉诱导前给予依达拉奉联合地塞米松,同时给予BIS监测,可提高胸腔镜下肺叶切除术后肺保护作用,促进术后恢复。     

Isolation, cloning and characterization of argF gene DNA from Escherichia coli K-12.

A 1650 base pair (BP) fragment carrying the entire argF structural gene with its associated control regions was isolated from an EcoRI/BamHI digest of phi80argFilambda cI857 DNA. This segment was cloned using the EcoRI and BamHI cleavage sites in the plasmid pBR322. A preliminary restriction map of the argF region was prepared. RNA polymerase binding studies indicated that the argF promoter is located approx. 30 base pairs from the EcoRI terminus of the cloned DNA segment.     

A two-dimensional polyacrylamide gel electrophoresis (PAGE) system using sodium dodecyl sulfate-PAGE in the first dimension.

A two-dimensional gel electrophoretic system for the separation of cellular proteins is described. The system utilizes sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in the first dimension and polyacrylamide gel isoelectric focusing in the second dimension. The system offers a good starting point for many difficult protein separations requiring SDS.     

超快通道麻醉联合BIS监测对心脏手术患者的效果及安全性

目的:探究超快通道麻醉辅助脑电双频指数(Bispectral index,BIS)监测对行心脏手术患者认知功能障碍的影响和安全性。方法:选取2014年1月-2017年1月于我院进行心脏手术的59例患者为研究对象,按照随机数字表法将其分为实验组(29例)和对照组(30例)。其中,对照组患者实施心脏超快通道麻醉,实验组患者实施心脏超快通道麻醉辅助BIS监测。术后6个月,使用韦氏成人智力量表对两组麻醉前后认知功能障碍情况进行比对,并比较两组术后6个月内并发症的发生率。结果:(1)两组术后6个月时智力测试得分对比差异无统计学意义(P0.05),各指数间对比差异也无统计学意义(P0.05);(2)实验组患者术后6个月内并发症发生率较对照组显著降低(P0.05)。结论:与单独使用超快速通道的患者相比,行全身麻醉心脏手术患者使用超快速通道麻醉辅助BIS监测麻醉及单用超快速通道对患者认知功能障碍的影响相当,但前者的安全性明显高于后者。     

Use of p-aminophenyl D and L-lactic acids and p-aminophenyl pyruvic acid as effectors in the affinity chromatography of lactate dehydrogenase.

p-Aminophenyl pyruvic acid and D-p-amino-phenyllactic acid were immobilized on a new synthetic acrylic carrier bearing acylating N-succinimidyl ester groups. The derivatives obtained were used successfully to purify lactate dehydrogenase (LDH) by affinity chromatography, the elution being carried out by means of NADH or preferably L-phenyllactic acid. Moreover, the specific activity of the LDH contained in a human blood serum was increased 270 times, using L-p-aminophenyllactic acid immobilized on a mixed polyacrylic agarose carrier.     

羟考酮和舒芬太尼对丙泊酚靶控输注时患者意识消失和BIS的影响

摘要 目的：采用羟考酮和舒芬太尼复合丙泊酚对患者进行TCI全麻诱导,考察对OAA/S和脑电双频谱指数（Bispectral index,BIS）的影响。方法：以2017年3月-2020年2月于我院行全麻插管手术的80患者为研究对象,采用随机数字表法分为对照组和研究组,各40例,对照组进行舒芬太尼联合丙泊酚TCI麻醉,研究组进行羟考酮联合丙泊酚TCI麻醉,考察不同丙泊酚Ce（0~2.5 μg/mL）时OAA/S值、不同时间（基础值、TCI开始前、患者意识消失时）BIS值、诱导持续时间以及意识消失时丙泊酚Ce浓度。结果：在丙泊酚Ce为0.5、1.0 μg/mL时,研究组OAA/S值显著高于对照组（P＜0.05）,在其他浓度下两组OAA/S值无显著差异（P＞0.05）;与0 μg/mL时的OAA/S相比,两组0.5~2.5 μg/mL时的OAA/S均显著降低（P＜0.05）。两组患者BIS基础值、TCI开始前和患者意识消失时的BIS值均无显著差异（P＞0.05）。两组患者诱导持续时间和意识消失时Ce均无明显差异（P＞0.05）。结论：羟考酮、舒芬太尼复合丙泊酚靶控麻醉诱导,均能引起OAA/S和 BIS值的降低,可增强丙泊酚镇静催眠作用,具有一定的协同作用。