广东汉族健康人载脂蛋白AⅠ-CⅢl-AⅣ基因簇DNA多态性及其单倍型分布

本文研究了中国广东汉族健康人群apoAI-CⅢ-AIV基因簇DNA限制性内切酶PstI、SstI和EcoRI片段长度多态性。其中等位基因P_1,P_2,S_1,S_2,R_1和R_2的频率分别为0.98,0.02,0.96,0.04,0.90和0.10。经卡方检验符合Hardy-Weinbery氏遗传平衡,与其他种族比较,本文结果显示中国广东汉族人P_2等位基因频率低于日本人、亚洲印第安人和高加索人,S_2等位基因频率低于日本人、菲律宾人、沙特阿拉伯人和亚洲印第安人,而与高加索人相近,R_2等位基因频率稍高于高加索人。不同种族间apoAI-CⅢ-AIV基因簇DNA多态频率无疑存在差异,这种差异可能是由于遗传漂变和自然选择单独或联合作用所致。对P_1、P_2,S_1、S_2和R_1、R_2构成的单倍型和连锁平衡程度进行了分析,结果显示这些单倍型处于连锁不平衡状态。     

Co-distribution of annexin VI and actin in secretory ameloblasts and odontoblasts of rat incisor

Summary Annexin VI and actin were detected by immunoblot analysis in the enamel- and dentin-related portions of dental tissues. Annexin VI was found mainly in the particulate fraction whereas actin was detected in both the soluble and particulate fractions. By immunoelectron microscopy, annexin VI antibodies conjugated with colloidal gold were seen to label the mitochondria, the cytosol and the nucleus of secretory ameloblasts and odontoblasts of rat incisor. In the processes of these cell, the plasmalemmal undercoat was labeled. Antiactin antibodies labeled the desmosome-like junctions, the cytosol, and the mitochondria of the cell bodies. Extensive labeling was seen at the periphery of the Tomes' processes and odontoblast processes. These results suggest that annexin VI may play a role in Ca²⁺-regulation in the cell bodies, especially as a calcium receptor protein in the mitochondria. Moreover, annexin VI and actin seem to be co-distributed in secretory processes. Thus, these proteins might be both involved in exocytotic and endocytotic events.     

亚硒酸钠对AH_(22)小鼠腹水型肝癌细胞中组织特异酶CPSⅠ的诱导及对细胞增殖酶ACT的抑制

通过连续四天腹腔给硒(1μgNa_2SeO_3/克体重)后,腹水型肝癌细胞中与细胞分化相关的CPS_(ase)Ⅰ活性显著上升,同时与细胞增殖相关的ACT_(ase)活性明显下降。而在正常鼠肝中,按相同方式给硒的结果是ACT_(ase)活性明显增高,CPS_(ase)Ⅰ活性则略有下降。该结果表明硒可能涉及对细胞分化与增殖的调控。     

人鼻咽癌细胞基因组中瘤基因Ha－ras－1的Dnase Ⅰ超敏感位点的初步研究

ＤＮａｓｅⅠ超敏感位点的研究能够发现潜在的调控基因转录活化的位点,比较正常人外周血有核细胞,淋巴瘤细胞株Ｐ３ＨＲ１和人鼻咽癌低分化磷癌细胞株ＨＯｎＥ１和ＨＮＥ２中Ｈａ－ｒａｓ－１瘤基因的ＤＮａｓｅⅠ超敏感位点发现,只有ＨＯＮＥ１和ＨＮＥ２细胞基因组中存在一个ＤＮａｓｅⅠ超敏感位点,位于第一个外显子上游０．３７ｋｂ处,上述结果提示正常白细胞和Ｐ３ＨＲ１细胞中Ｈａ－ｒａｓ－１基因处于失活状态,而在鼻咽癌细胞基因组中则处于活化状态,它的活化可能与０．３７ｋｂ处的ＤＮＡ序列有密切的关系。     

Functional and genetic analysis of annexin VI

This study is concerned with the determination of the function of the 68kDa calcium-binding protein, annexin VI. Studies on the structure and regulation of the gene include a detailed analysis of annexin VI expressed heterologously in human A431 carcinoma cells. We have recently discovered that annexin VI is subject to a novel growth dependent post-translational modification. Interestingly, the protein exerts a negative effect on A431 cells. This effect was manifested as a partial reversal of the transformed phenotype. We are currently exploring the hypothesis that the post-translational modification of annexin VI is required for sub-cellular targeting, and that correct localisation within the cell is essential for function.     

羊角天麻中的两个新倍半萜

羊角天麻中的两个新倍半萜刘绍华,程菊英（广西植物研究所,桂林５４１００６）吴大刚（中国科学院昆明植物研究所植物化学开放实验室,昆明６５０２０４）关键词羊角天麻;倍半萜;羊角天麻素Ⅰ;羊角天麻素ⅡＴＷＯＮＥＷＳＥＳＱＵＩＴＥＲＰＥＮＥＳＦＲＯＭＤＯＢＩ...     

基于线粒体基因分析蓝孔雀与绿孔雀的遗传差异

种间的遗传差异是物种分类和确定保护管理单元的基础,本研究利用DNA条形码技术对未知样本进行鉴定,通过NCBI进行BLAST得到结果是:与绿孔雀的同源性为96%。近一步通过对蓝孔雀(Pavo cristatus)和绿孔雀(Pavo muticus)线粒体细胞色素C氧化酶Ⅰ(cytochrome coxidaseⅠ,COⅠ)基因及线粒体基因组的比较分析,结果表明两物种间的COⅠ基因在碱基组成、核苷酸多样性等各项指标上均具有明显差异。遗传距离分析结果表明蓝孔雀与绿孔雀种内遗传距离为分别0和0.012,种间遗传距离为0.045,表明种间仍具有明显的遗传差异。通过对两物种线粒体基因组各基因的比较分析,发现ND1基因变异位点所占比例相对较高,考虑作为绿孔雀和蓝孔雀种群遗传学研究的最优分子标记。本研究将为分析孔雀类群间的系统发育及制定绿孔雀的保护措施提供了更多科学依据。     

丙型肝炎病毒受体SR-BⅠ的研究进展

丙型肝炎病毒（HCV）是经血液传播而引起急、慢性肝炎的主要致病因子之一,是导致肝硬化、肝细胞癌等终末期肝病的主要原因。位于HCV包膜E2蛋白N端的第1高变区（HVR1）,是介导E2蛋白与B族I型清道夫受体（SR-BⅠ）结合及HCV感染细胞的关键肽段。研究表明,HCV可能利用了SR-BⅠ受体的某些生理功能入侵细胞,进行细胞-细胞间传播。因此,HVR1与SR-BⅠ相互作用的研究除了能深入了解HCV吸附和入侵细胞机制,同时也为治疗和预防HCV感染提供了新的靶点。     

Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells

In the present study, we used a functional proteomic approach to identify Annexin A1 (Anxa1) interacting proteins in the Philadelphia‐positive KCL22 cell line. We focused on Anxa1 because it is one of the major proteins upregulated in imatinib‐sensitive KCL22S cells versus imatinib‐resistant KCL22R. Our proteomic strategy revealed 21 interactors. Bioinformatic analysis showed that most of these proteins are involved in cell death processes. Among the proteins identified, we studied the interaction of Anxa1 with two phosphatases, Shp1 and Shp2, which were recently identified as biomarkers of imatinib sensitivity in patients affected by chronic myeloid leukemia. Our data open new perspectives in the search for annexin‐mediated signaling pathways and may shed light on mechanisms of resistance to imatinib that are unrelated to Bcr‐Abl activity. All mass spectrometry data have been deposited in the ProteomeXchange with identifier PXD000030.     

An inside job: Annexin 1A-Inositol 1,4,5-trisphosphate receptor interaction conveys endoplasmic reticulum luminal Ca2+ sensitivity

Cytoplasmic Ca²⁺ is a pivotal regulator of IP₃R activity. It is however controversial whether the [Ca²⁺] in the Endoplasmic Reticulum lumen also directly regulates channel function. We highlight a recent paper that demonstrates that luminal [Ca²⁺] potently inhibits IP₃R activity. This regulation occurs indirectly by an interaction mediated through a binding partner, likely Annexin 1A.