应用RAMP分子标记探讨拟鹅观草属的种间关系

采用RAMP (random amplified microsatellite polymorphism) 标记技术, 分析了拟鹅观草属9种1亚种和鹅观草属6 种植物之间的遗传变异和亲缘关系。33 个引物组合产生的310 条DNA扩增片段中, 286条(92 25%) 具有多态性, 每个引物组合产生5~13条多态性带, 平均为8 67条。利用310个RAMP标记, 在NTSYS pc软件中, 计算Jaccard遗传相似系数, 建立UPGMA聚类图。结果表明: (1) 物种间遗传差异明显, 具有丰富的遗传多样性; (2) 阿拉善鹅观草和大丛鹅观草与拟鹅观草属的物种聚类在一起, 表明它们与拟鹅观草属的亲缘关系较近, 而与本试验所分析的另外4个鹅观草属物种的亲缘关系较远; (3) RAMP分子标记可以将拟鹅观草属的物种分开, 而且形态相似、地理分布相同或相近的物种聚类在一起;(4) RAMP结果与形态学和细胞学的分析结果一致, 表明RAMP标记是评价拟鹅观草属种间关系十分有效的方法。     

核基因突变引起的拟南芥菜叶片花斑及其机制

细胞核基因突变引起的植物叶片花斑,是研究细胞器（特别是叶绿体）和细胞核之间信息交流的重要材料,也在园艺科学上有重要的应用价值。综述了拟南芥菜IM．VAR1、VAR2、CHM．CUE1、PAC．ATD2和VAR3等8个细胞核基因突变后引起的叶片花斑,主要包括这些基因所编码的蛋白质以及它们突变以后引起花斑的机制。     

不同理化因子对雪莲毛状根生长和总黄酮生物合成的影响

在 1 2MS液体培养基上研究了不同理化因子对水母雪莲毛状根生长和总黄酮生物合成的影响。实验结果表明 :氮源总浓度 (包括NH+4和NO-3)为 30mmol L ;NH⁺₄ NO^-₃比例为 5∶2 5 ;2 %蔗糖和 3%葡萄糖组合 ;0.5mg LGA₃和 0.5mg LIBA ;pH5.8;18h d的光照 (光强为 35.0.0lx) ;2.4℃ ;摇床转速为 100rmin有利于毛状根生长及总黄酮的生物合成。在此培养条件下 ,经过21d的培养毛状根生长量达到 12.8g L(DW) ,总黄酮合成量为 192.2mg L ,即总黄酮含量占毛状根干重的 15 % ,约为干重野生水母雪莲植株总黄酮含量的 2.5倍。     

多维协变量具有测量误差的结构回归模型

提出具有测量误差的结构回归模型,研究可交换条件下多维协变量的测量误差对平均处理效应估计的影响,在没有其它的附加条件下,尽管大多数模型参数不可识别,平均处理效应仍可识别,由于平均处理效应的极大似然估计求解困难,建议在实际中使用拟极大似然估计作为替代。     

中国拟菱纹叶蝉属分类研究(半翅目,叶蝉科,殃叶蝉亚科)

记述拟菱纹叶蝉属Hishimonoides Ishihara 2新种,锯缘拟菱纹叶蝉H.dentimarginus sp.nov.和侧突拟菱纹叶蝉H.laterosporeus sp.nov.给出了分种检索表.模式标本保存在贵州大学昆虫研究所.     

小刺甲属系统学研究(鞘翅目,拟步甲科)

对中国及其毗邻地区的小刺甲属Myatis Bates,1879进行了系统学研究,共记录7种,绘制了新种和已知种的特征图和的成虫照片,编制了世界小刺甲属已知种检索表,并对该属的系统进化关系进行了初步的支序分析.标本保存在河北大学博物馆.新种描述如下.     

中国贞琵甲属昆虫及一新种记述(鞘翅目:拟步甲科)

对中国贞琵甲属Agnaptoria Reitter,1887进行了初步总结,描述西藏1新种：芒康贞琵甲Agnaptoria markama,sp．nov．,给出了贞琵甲属已知种检索表。模式标本保存于河北大学博物馆。     

中国拟带叶蝉属分类研究(半翅目:叶蝉科:殃叶蝉亚科)

记述拟带叶蝉属Scaphotettix 5新种：红纹拟带叶蝉S.redstripeus．sp．nov．,刺茎拟带叶蝉S．splinterus,sp．nov．,长板拟带叶蝉Slongistyleus,sp．nov．．细纹拟带叶蝉S．slenderus,sp．nov．和梵净拟带叶蝉S．fanjingensis,sp．nov．,1中国新记录种：印度拟带叶蝉Sindicus（Distant）。模式标本分别保存在贵州大学昆虫研究所（IEGU）和西北农林科技大学昆虫博物馆（NWSUAF）。     

Seasonal changes of resistance to fenvalerate and cypermethrin in the larval parasitoid Cotesia plutellae （Hymenoptera： Braconidae）

The seasonal changes of insecticide resistance and stability in hymenopteran Cotesia plutellae, collected from Jianxin, Fuzhou-City, and Shangjie, Minhou-County, Fujian, China, were assessed by using a dry residual film method. The resistance to two insecticides in the field populations of C. plutellae was not stable under insecticide-free conditions in the insectarium. Compared with susceptible F11 progeny of C. plutellae in the insectarium, the resistance ratios （RR） in F0 parents were 18.4 for fenvalerate and 11.4 for cypermethrin based on LC50 at 9 hours, and 32.8 for fenvalerate and 28.5 for cypermethrin based on LC50 at 24 hours when the parasitoids were left in contact with the insecticides for 1 hour and mortalities were recorded at 9 and 24 hours, respectively. However, the RR in a field population of C. plutellae were 9.2 for fenvalerate and 12.7 for cypermethrin, if the parasitoids were left in contact with the insecticides for 24 hours. The resistances to the two pyrethroids in other field populations collected from Jianxin and Shangjie from November 2000 and July 2004 were also determined. Significant seasonal variations of resistance to the two insecticides in the field populations of C. plutellae were found. The RR were 3.0-18.4 for fenvalerate and 4.8-20.6 for cypermethrin in Jianxin populations from November 2000 to April 2002 based on LC50 at 9 h, and 2.3-13.6 for fenvalerate and 3.6-16.0 for cypermethrin in Shangjie populations from May 2002 to July 2004 based on LC50 at 24 hours. The resistance levels were high in spring and autumn and decreased sharply in summer. In addition, significant recovery from the knocked-down caused by the insecticides was found in the F0 and field populations of C. plutellae which were resistant to fenvalerate and cypermethrin if the parasitoids were left in contact with the pyrethroids for 1 hour. However, no recovery was found in susceptible F11 progeny.     

利用改进的Cap-trapper法构建拟斯卑尔脱山羊草(Ae.speltoides Tausch)全长cDNA文库

Captrapper法是目前全长cDNA文库构建的重要方法之一。在引进、消化、吸收的基础上,通过设计引物,同位素检测,对末端转移反应条件控制等方面做了一些切实可行的改进,形成了一套简单易行的技术体系。利用改进的Captrapper方法,成功构建了小麦B基因组的可能供体种拟斯卑尔脱山羊草(Ae.speltoides)的全长cDNA文库。经综合评价,文库的全长比例达到89.6%,重组率为99%,库容量超过3.0×106cfu。