植物蛋白的体外泛素化检测方法

泛素激活酶(E1)、泛素耦联酶(E2)和泛素连接酶(E3)是蛋白质泛素化修饰的关键酶。在真核基因组上有大量基因编码这些泛素化相关的酶类或蛋白。检测这些泛素化修饰酶及其底物蛋白的生化特性和特异性是分析其生物学功能的重要内容。该文提供了一种简便快速检测体外泛素化反应的方法, 不仅可通过检测对DTT敏感的硫酯键的形成来判断E2的活性、检测E3的体外泛素化活性, 而且可以检测E2-E3和E3-底物的特异性。所用蛋白主要来源于拟南芥(Arabidopsis thaliana), 包括分属于绝大多数E2亚家族的成员, 可用于不同RING类型E3的活性检测。该方法不仅可以采用多种E2进行E3活性分析, 而且可以分析不同组合的E2-RING E3、RING E3-底物的泛素化活性等, 亦可应用于真核生物蛋白质尤其是植物蛋白的体外泛素化活性分析。     

长链非编码RNA基因Ef-cd调控水稻早熟与稳产

水稻(Oryza sativa)育种中早熟和高产往往不能兼顾。最近一项研究分离鉴定了1个调控水稻早熟的基因Ef-cd (Early flowering-completely dominant)。Ef-cd编码一段长链非编码RNA (lncRNA), 该lncRNA与OsSOC1基因的一段反义转录本重叠, Ef-cd正调控H3K36me3的富集及OsSOC1基因的表达。在不同的纬度, 含有Ef-cd基因的品种及其杂交种与对照相比生育期缩短7-20天, 但产量不降低。对1 439份优质杂交稻品种的基因型分析显示, 含有Ef-cd基因的16份纯合子和299份杂合子均表现出明显的早熟特性。Ef-cd基因可能通过提高氮素利用率及光合效率来促进水稻早熟。因此, Ef-cd基因兼顾了水稻的早熟和产量, 在水稻分子育种中具有重要利用价值。     

基于转录组分析鉴定苹果茉莉素响应基因

为阐明外源茉莉酸甲酯(MeJA)诱导的苹果(Malus domestica)抗病分子机制, 以生长30天的Gala组培苗为试材, 用100 μmol?L ^-1MeJA处理叶片12小时, 通过转录组测序, 结合生物信息学分析鉴定出苹果叶片中受MeJA诱导表达的基因。结果表明, 外源MeJA主要影响苹果叶片倍半萜类、三萜和类黄酮的生物合成, 以及芸薹素(BR)信号转导途径间接诱导的抗病性; 倍半萜类、三萜及类黄酮生物合成途径中的关键基因为MDP0000702120和MDP0000692178; MDP0000123379是联系芸薹素信号转导途径和植物-病原菌互作途径的关键调控基因。     

梭鱼幼鱼选择温度的研究

梭鱼幼鱼选择温度的研究张梅英岳玉环（中国医科大学实验动物部,沈阳１１０００１）（吉林农业大学动物科学系,长春１３０１１８）侯文礼（东北师范大学环境科学系,长春１３００２４）ＴｅｍｐｅｒａｔｕｒｅｓｅｌｅｃｔｉｏｎｆｏｒＬａｒｖａｏｆＬｉｚａｈａｅｍａ...     

Changes of macromolecular organizations in nonjunctional sarcolemmas after cross-innervation──a study offast-and slow-twitch muscle fibres in rats

INTRODUCTIONMa1n11la1iaIlskeletal1llusclefibresare(listiIlgl1ishedint()severaltypesacc()rdillgtodifferentclassificatiollscl1e111es.Eachtypeofmusc1efibresl1asasetofspecificcharacteristics.Ollewell-kllowl1waytoclassifymusclefibresistodividethemintotwobroadtypestslow-twitcI1ortypeIfibres,andfast-twitchortypelIfibres.ThefOrmerllormallyappearsreda11dthelaterappearswhite.Theypossesswidedifferellceswithrespectt()physiol()gical,biochemical,andmorpho1ogica1pl1ellotypiccharacteristics,suchasisom…     

A NEW HYOPSODUS FROM THE EARLY EOCENE OF FANGXIAN, HUBEI

CondylarthraCoPe,l88lHyopsodontidaeTrouessart,l879HyopsodusLeidy,l87oHyopsodusfangxianensissp.nov.TypeArightmendibularfragmelltpreservingml-ln3(IVPPVl2oo5).LocalityandhorizonLuojiagou,Fangx1anCou11ty,HubeiProvince;EarlyEoceneYoupingFormation.Diagnosism24.5mmlong.Thelel1gthofm1a11dm2ncar1yequal.m1andm2re1ativelywideafldIObustwhilem3narrowands1cn(ler.Noparaconidandme-tasty1idonthemo1ars.Hypoconu11dmoredevelopedandsituatednearthemiddleofthetoothonthefirsttwolowermolars-EtymologyTrivia…     

THE GEOLOGICAL SIGNIFICANCE OF SINACANTHUS FROM TARIM, CHINA

TheSinacanthusfossiIlocalitieswererestrictedtotheYangtzeregionbeforethediscoveryoftheTarimfossils.TheyusedtobereferredtoasDevonianorEarlyDevonianinage,butlaterdiscoverieshavedemostratedthattheageofSinacanthus-bearingbedsshou1dbeSilurian(Li,198O).In1988,fiveSinacanthusfossill,edswerefoundintheTataertagPormationofKalping,NWTarimandtheageoftheTataertagFormationwasconsideredMiddle-LateSilurian(Table2).Inthesummerofl992,WangJunqing,ZhuMinandtheauthorofthispaperfoundalotoffossilfinspinesi…     

STABLE POSITIVE SOLUTION OF THE NONAUTONOMOUS LOTKA-VOLTERRA MUTUALISTIC SYSTEM

l！ntroductionAutonomousLotka－VolterramutuallstlcsystemhasbeenstudiedInmanypaperE3】,E4］．［5】,ourpurposehereIstoconsiderthenonautonomouStwo－SPPCllsLOtkt－Volterramutuallstlcsystem．WeareconcernedwiththedifferentialequationsbelongtoC‘andC‘isthesetOfsllCOlltlnuouSfunCtionSg：［0,＋。）、RbOUnded。hove。ndbelOWbyCOnst。ms,moreover,notallofa：。（t）（l＜l,j＜n,。／j）arezero．Theper。odlccasehasbeenconsideredIn［1】．Insection3ofthlspaper,westudiedthegeneralcaseoftlmede…     

ANALYSIS OF GENETIC CORRELATIONS FOR QUALITY TRAITS IN INDICA RICE

INTRODUCTIONTherearesomekindsofcorrelatedrelationshiPsbetweenagronomytraitsIntndtcarice．Geneticandenvlronmentatcorrelat［onshavebeenanalyzedforsometraitsInrice［‘·’J．LiandYangfoundadditivecorrelationcoefficientswerefrom一0．773to0·993forsomeflowertraitsInrice［’］．Yuhavefoundthataddltlvecorrelationwasslgnlflcantforsometraitsinbarley［13］．Uptonow,partitioningthegenotyplccovarlanceoftraitsInricehasnomorethanaddltlveanddominancecovarlancecomponents．kcausethenutrl－＊＊t吕Of凡8…     

NEW MATERIALS OF DINOCROCUTA GIGANTEA FOUND IN FUGU COUNTY, SHAANXI PROVINCE

Theskullisverybigandrobust,exceptionallyshortandwidthinproperty.Theroofascendslikeaflightofsteps.Occipitalistriangleinformandupperborderextendsposteriorly,projectedovertheoccipital.Theanteriorpartofupperpremolariswiderthanthatofposteriorone.TheprotoconeofP4isverysmall.LengthofM,islongerthanthatofP4.ItslimbswerediscoveredforfirsttimeinChina.Itisshownafterstudyingthatitsskullisexceptionallyrobustbutitslimbsdonotindicateanyrobust.ComparedwiththeHyaenasinensis,theskullofDinocrocutagiganteaisl.…