一株H5N1亚型禽流感病毒的分离与鉴定

2004年1月湖北宜昌某鸡场暴发疫病,从该鸡场濒死鸡肺组织中分离到了一株病毒,电镜切片观察到典型的禽流感病毒粒子;采用ELISA检测禽流感抗原为阳性;RT-PCR扩增HA、NA基因并测序,经BLAST分析,HA基因与A／Goose／Guangdong／1／96(H5N1)HA基因同源性为97％;NA基因与A／Goose／Guangdong／1／96(H5N1)NA基因同源性为96％,确定该分离株为禽流感病毒H5N1亚型(A／Chicken／Yichang／Lung-1／04(H5N1))。     

阳桃胚乳愈伤组织诱导和不定芽发生的研究

首次成功建立阳桃胚乳组织培养并获得胚乳再生植株。胚乳愈伤组织诱导以培养基MS 2,4-D2．0mgL^-1 BA0．2mgL^-1的效果最好,诱导频率可达94．7％,愈伤组织乳白色,结构致密,生长旺盛;将其接种在培养基MS ZT3．0mgL^-1 NAA0．2mgL^-1上,愈伤组织由乳白色致密型转变为淡绿色致密型,进而形成绿色芽点,分化出不定芽,分化频率可达73．3％;胚乳植株在培养基MS ZT2．0-2．5mgL^-1 NAA0．05mgL^-1上进行壮苗和营养繁殖。     

抗菌肽的研究进展

抗菌肽又称抗微生物肽(antimicrobial peptide)或肽抗生素(peptide antibiotics),在动植物体内分布广泛,是天然免疫防御系统的一部分。抗菌肽不仅有广谱抗细菌能力,而且对真菌、病毒及癌细胞也有作用。对抗菌肽作用机理的研究是近来的热点之一,本文综述了此方面近来的进展,并对微生物针对抗菌肽的耐药性进行了讨论。     

对(A)KTAexplorer100快速纯化工艺开拓系统的几点认识

生物分子纯化方法是生物技术的重要组成部分,为优选纯化过程,选用介质和探索操作条件是形成完整而又合理纯化方案的两个方面：AEKTAexplorer100快速纯化工艺开拓系统为之提供了操作平台。本文介绍了它的特性、有关参数和作者的体会。     

(A)KTAexplorerl00测定蛋白质相对分子质量的实验分析

讨论用(A)KTAexplorerl00快速纯化工艺开拓系统进行凝胶层析测定蛋白质相对分子质量所涉及的限定条件问题.对该系统有关特性测试及应用表明,它具有加样量少、重复性好、定性和定量准确、快捷方便等优点.     

柠檬形克勒克酵母对温州蜜柑“国庆一号”采后贮藏的防腐效果

Citrus unshiu “Guoqing No.1” was used as material for elucidating the effect of Kloeckera apiculata suspension post-harvest treatment on preventing fruit decay during storage period. The result showed that K. apiculata preparation of 2×106CFU/mL (CFU, cloning forming unit) significantly reduced the development of decay caused by green mold, blue mold and stem rot, showing the same effect of carbendazim treatment after storage period of 100 days, and the disease incidence was controlled under 1%. The antagonist population was between 3.5×105CFU/mL and 1.7×107CFU/mL in citrus fruit, and increased 1.7×107CFU/mL after 40 days storage, and then stabilized for the remaining storage period. After storage at 5℃ for 100 days, K. apiculata strain 34-9 did not alter any quality parameters of fruit. During storage, the change of relative electrical conductivity (REC) showed a coordinate tendency with malondlaldehyde (MDA) content, which kept continuously growing from the beginning to the end of the storage. In the storage period of 25 days, the REC value and MDA content of the treatments were all significantly lower than those of water treatment, but had no obvious difference from those of carbendazim treatment. K. apiculata strain 34-9 had no effect on the enzyme activity of super oxide dismutase (SOD), but significantly improved peroxidase (POD) activity after 100 day storage.     

Ⅰ型胶原基因启动子研究进展

Ⅰ型胶原由α1（Ⅰ）链（COL1A1）与α2（Ⅰ）链（COL1A2）组成,人COL1A1与COL1A2基因的启动子都已鉴定并克隆。调节转录的核因子有Sp1、NE－1、AP－1、CBF、NF－κB、C－myb、c-Krox、BFCOL1、Zf9、Sp3、Smads、TGP、Egr-1、TWIST与NP／NMP4等。在COL1A1和／或COL1A2基因启动子上已鉴定出TGFβ、糖皮质激素、TNFα、IFNγ与视黄酸等的应答元件。     

碳氮源对转基因鱼腥藻Anabaena sp.PCC7120培养的影响

对碳源、氮源种类和用量对转rhTNF-α基因鱼腥藻7120（Anabaena sp.PCC7120）培养的影响进行了研究,发现最适碳源为蔗糖,最适氮源为NaNO3,最佳用量分别为9g/L和2．25g/L,此时生物量远高于自养方式,达2．52g/L,比相同条件下在BG－11培养基培养高71．66％,ATNF－α表达量为16％－22％,生物活性为10^5U/mg。     

南蛇藤种油中的倍半萜成分

从南蛇藤种油中分离了六个β－二氢沉香呋喃倍半萜,通^1HNMR,^13和单晶X－线射线衍射鉴定,它们的结构是：1α,6β－二乙酰氧基－9β－苯甲酰氧基－β－二氢沉香呋喃（1）,1α,6β－二乙酰氧基－9β－肉桂酰基－β－二氢沉香呋喃（2）,1α－乙酰氧基－6β,9β－二苯甲酰氧基－β－二氢沉香呋喃（3）,1α,2α－二乙酰氧基－9β－肉桂酰氧基－β－二氢沉和时呋喃（4）,1α－羟基－2α－乙酰氧基－9β－肉桂酰氧基－β－二氢沉香呋喃（5）和1α－乙酰氧基－2α羟基－9β－肉桂酰氧基－β－二氢沉香呋喃（6）。化合物2,5和6是首次从南蛇藤中分离得到。