| 一株H5N1亚型禽流感病毒的分离与鉴定 |
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| 引用本文： | 寇铮,唐利军,雷富民,安学芳,范兆军,李天宪.一株H5N1亚型禽流感病毒的分离与鉴定[J].Virologica Sinica,2004,19(5):490-492. |
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| 作者姓名： | 寇铮 唐利军 雷富民 安学芳 范兆军 李天宪 |
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| 作者单位： | [1]中国科学院武汉病毒研究所，湖北武汉430071 [2]中国科学院动物研究所，北京100101 |
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| 基金项目： | 国家科技攻关计划项目(2004BA519A11) |
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| 摘 要： | 2004年1月湖北宜昌某鸡场暴发疫病，从该鸡场濒死鸡肺组织中分离到了一株病毒，电镜切片观察到典型的禽流感病毒粒子；采用ELISA检测禽流感抗原为阳性；RT-PCR扩增HA、NA基因并测序，经BLAST分析，HA基因与A／Goose／Guangdong／1／96(H5N1)HA基因同源性为97％；NA基因与A／Goose／Guangdong／1／96(H5N1)NA基因同源性为96％，确定该分离株为禽流感病毒H5N1亚型(A／Chicken／Yichang／Lung-1／04(H5N1))。
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| 关 键 词： | 禽流感病毒 H5N1 |
Isolation and Identification of anAvian Influenza Virus of Subtype H5N1 from Hubei Province |
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| KOU Zheng,TANG Li-jun,LEI Fu-min,AN Xue-fang,FAN Zhao-jun,LI Tian-xian.Isolation and Identification of anAvian Influenza Virus of Subtype H5N1 from Hubei Province[J].中国病毒学(英文版),2004,19(5):490-492. |
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| Authors: | KOU Zheng TANG Li-jun LEI Fu-min AN Xue-fang FAN Zhao-jun LI Tian-xian |
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| Institution: | KOU Zheng,TANG Li-jun,LEI Fu-min,AN Xue-fang,FAN Zhao-jun,LI Tian-xian1** 1 1 2 1 1 |
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| Abstract: | A strain of avian influenza virus was isolated from lung tissue in 2004. It was determined to be a Avian influenza virus by ELISA and electron microscopy. HA and NA gene was amplified by RT-PCR and sequenced. By the analyses of HA and NA gene sequences, the isolate was an H5N1 subtype of avian influenza virus (A/Chicken/Yichang/Lung-1/04(H5N1)). |
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| Keywords: | Avian influenza virus H5N1 |
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