难辨梭菌性肠炎与肠菌群失调的关系

本文对11例难辨梭菌性肠炎(CDEC)患者的粪便进行10种肠道菌的定量分析,并与20例其他原因腹泻病人和22例健康成人的肠菌比较。结果表明CDEC病人的粪中大肠杆菌和双歧杆菌明显低于两对照组,其他菌类与对照组无明显差别,提示大肠杆菌和双歧杆菌的减少在CDEC发病中起重要作用。此外,我们对CDEC病人发病前所用抗生素作了统计分析,结果发现:使用较多的有庆大霉素、氨苄青霉素和先锋霉素     

用定点突变技术研究转录终止——rpoBC操纵子上~tL7茎环结构的作用研究

 E.coli RNA聚合酶ββ'亚单位编码的基因rpoBC与核糖核蛋白基因rplJL共同构成rpoBC操纵子。rpl-rpo间区转录终止信号~tL7的转录产物RNA有两组富含C-G的反向对称结构及一串寡聚U;反向对称区可形成1:2和3:4茎环或单一5:6茎环。 用M13mp11噬菌体插入~tL7的112bp片段重组M13mp11-490,在此基础上用定点突变技术建立~tL7的七核苷酸缺失突变体,从而破坏1:2茎环,建成M13mp11-85。 分别把原型~tL7及缺失型~tL7~v克隆到表达质粒pHR24中,构建成pHR37(P_ftsQ~tL7-galk)及pHR24-9(p_ftsQ-~tL7~v-galk)。测定galk基因产物半乳糖激酶活性,推算转录的终止效率。结果表明:~tL7终止效率为50％,~tL7~v为20％。说明仅有3:4茎环及寡聚U,即具终止作用; 1:2茎环通过某种方式加强3:4茎环从而提高终止效率。     

The effect of insecticides on the distribution of foracing parasitoids,Diaeretiella rapae [Hym.: Braconidae] on plants

Foraging aphid parasitoids,Diaeretiella rapae M'Intosh, were exposed to sublethal doses of the insecticides pirimicarb, permethrin and malathion on brusslls sprouts plants. Observations on wasp distribution over time revealed that wasps spent less time on sprayed plants, relative to controls and, while on these plants, tended to concentrate activity on unsprayed surfaces. For permethrin and malathion, pesticide residues reversed the stereotypic upward foraging pattern of the wasp. Negative consequences of sublethal pesticide doses for parasitoid foraging efficiency are discussed.      

复方克痛宁临床应用镇痛效果

应用复方克痛宁注射液,治疗各种痛证８２例,显效５０％,有效４３．９％,总有效率９３．９％。其中大多数病例都是经用其它药或疗法无效或效差而用本药获效的。对关节痛、神经血管性头痛等顽固性疼痛显著独特。用药次数最少１次,最多３５例,仅极个别有轻微短暂口干、头晕副作用,各项常规化验检查均无异常,效果显著高于克痛宁或盐酸平痛新单用。临床应用的结果与动物试验观察到的协同增效结果一致。     

广赤眼蜂滞育诱导中的种内变异及复寄生的作用(英语)

研究了采自伊朗不同害虫的两种广赤眼蜂种群(T.evanescens A和T.evanescens B)的滞育,在米蛾卵内用恒温8℃,5℃或变温(11-23)℃-11℃容易地诱导T.evanescens A进入稳定的滞育,但却不能用同样的温度和寄主诱导T.evanescens B进入滞育.试验表明,母代的寄主和避免复寄生的能力可能在广赤眼蜂滞育诱导种内变异中起重要作用.     

海南省库蠓二新种(双翅目:蠓科)(英文)

本文描述在海南省发现的库蠓二新种:尖峰库蠓和开裂库蠓。     

云南白蛉亚科昆虫的研究——四、贵真司蛉Sergentomyia(Neophlebotomus)kueichenae sp.nov.的描述(双翅目:毛蛉科)(英文)

1989年8月5—14日,作者等在云南省西双版纳勐腊县易武(5—9日)和勐远(12—14日)的自然石灰岩洞中捕获了大批白蛉。已定种名的有:长铗异蛉Idiophlebotomus longiforeps,云胜白蛉Plebotomus yunshengensis,上门白蛉P. tumenensis,江苏白蛉P. kiangsuensis,施氏白蛉P. stantoni,卢氏司蛉S. rud nicki,鲍氏司蛉S. barraudi,应氏司蛉S. iyengari,歌乐山司蛉S. koloshanensis和贝氏司蛉S. bailyi。此外有新蛉亚属一新蛉种,为对我国医学昆虫学专家李贵真教授的学术贡献表示敬意而名之为贵真司蛉Sergentomyia(Neophlebotomus)kueichenae。 文中对贵真司蛉做了详细描述,并与相近蛉种:应氏司蛉和坦博司蛉S. tambori进行了对比鉴别。正模(雌蛉):勐远岩洞,北纬21°24′,东经101°30′,海拔约1000m;副模:勐远岩洞及易武岩洞(海拔约1400m),皆存于暨南大学医学院寄生虫学教研室。     

八角科植物的地理分布

本文依据八角科的系统分类和地理分布,结合古植物、古地理和古气候资料,分析和推论八角科的起源地点在劳亚古陆,很可能是在劳亚古陆中南部的温暖湿润山地。八角科的起源时间早于白垩纪末期,很可能在白垩纪中期。八角科的迁移扩散途径是沿山地进行,从西欧进入北美,从北往南,从内陆往沿海。世界现代八角科植物是以东亚成分为主,东亚的横断山至华东一带（２０—３０°Ｎ,９８—１２３°Ｅ）为八角科的现代分布中心、现代分化中心和东亚八角科现代原始类群分布中心。八角科曾为古热带湿润山地分布型,现代为东亚－北美分布型;现代分布格局形成的原因在欧美是因为海浸和冰川作用,在亚洲则是寒化（冰川）和旱化的综合作用,而物种丰富程度则是由于东亚较北美有更多的地理隔障机制。     

Pattern recognition analysis to the variation of nasal-pharynx cancer patients' trace element levels in samples of hair,whole blood,and tissue

Pattern recognition has been used in this paper to analyze trace element levels in patients diagnosed with nasal-pharynx cancer (NPC) and in healthy control subjects. Trace elements such as Zn, Cu, Fe, Mn, and Mg have been tested in samples of hair and whole blood. Through Mahalanobis Distance Decision analysis, we have achieved good classification effects in whole blood samples: Efficiency for distinguishing patients is 96% and that of healthy controls is 90%. Classification hair samples is inferior to whole blood: Decision accuracy for patients is 58% and healthy controls is 90%. These results are also shown in a nonlinear mapping figure. At the same time, we have also determined 5 trace element levels in 16 other cancer patients' nonneoplastic and cancerous tissue, with no significant difference between them: Decision accuracy of cancerous tissue is 63%, and in nonneoplastic tissue is 50%, hence, we cannot identify them. It can be inferred that there is no idiosyncratic change of trace elements in cancer patients' neoplastic tissue, the change of a cancerous person may occur in the whole body.     

AST同工酶的琼脂糖凝胶电泳NBT显色法

分布于细胞内线粒体及细胞质中的天门冬氨酸氨基转移酶(AST·EC·2·6·1·1)是人血清中含有的两种同工酶,分别称为AST-m和AST-c同工酶.AST-c电泳迁移率介于血清α-球蛋白与β-球蛋白之间.AST-m电泳迁移率相似于γ-球蛋白,琼脂糖凝胶电泳固蓝B染色法对m-AST检出率较低.用NBT显色法则可得到较好效果.