低浓度CO2对水车前和黑藻光合特性的影响

本实验通过对沉水植物水车前（offelia alismoides）和黑藻（Hydrilla verticillata）进行低浓度C02诱导,研究其光合特性的变化。研究显示,诱导后,水车前的PEPC／Rubisco活性比值由0．45上升到4．17,黑藻由0．47上升到4．17,两种植物的C4途径光合酶PEPC和NAD—ME的活性升高了10倍左右,其他C4光合酶如NAD—MDH和PPDK的活性也大幅升高;诱导后,水车前和黑藻的其他光合特性也发生了显著变化：净光合速率比对照分别提高了50．8％和74．1％,O2对处理组光合速率的抑制分别为对照组的35％和60％,叶绿素荧光基本参数也有显著性变化。研究结果表明,经低浓度C02诱导,水车前和黑藻光合碳同化途径可能由C3转变成为C4。     

扁桃幼果发育过程中的内源激素含量变化

用高效液相色谱测定扁桃幼果发育过程中脱落与否的果实中内源生长素(IAA)、赤霉素(GA3)和脱落酸(ABA)含量变化的结果表明,幼果发育初期,脱落幼果中IAA和GA3含量均明显低于未脱落的,而ABA含量则明显高于未脱落的果实。     

窄柄姬蜂属(膜翅目,姬蜂科)在中国首次发现

报道在中国首次发现的窄柄姬蜂属Calaminus Townes,1965并1新纪录种:北海道窄柄姬蜂Calaminus hokkaidensis(Uchida,1930).     

中国的耕姬蜂属(膜翅目,姬蜂科)及一新种

记述采自河南省宝天曼自然保护区的耕姬蜂属Arotes Gravenhorst,1829的新种,斑耕姬蜂Arotes moculatus sp.nov.和采自辽宁及吉林的1已知种:白带耕姬蜂A.albicinctus albicinctus Gravenhorst,1829.编制了耕姬蜂属中国已知种检索表.     

Retracted: IRAK3 gene silencing prevents cardiac rupture and ventricular remodeling through negative regulation of the NF-κB signaling pathway in a mouse model of acute myocardial infarction

Cardiac rupture and ventricular remodeling are recognized as the severe complications and major risk factors of acute myocardial infarction (AMI). This study aims to evaluate the regulatory roles of interleukin-1 receptor-associated kinase 3 (IRAK3) and nuclear factor-κB (NF-κB) signaling pathway in cardiac rupture and ventricular remodeling. Microarray analysis was performed to screen AMI-related differentially expressed genes and IRAK3 was identified. The models of AMI were established in male C57BL/6 mice to investigate the functional role of IRAK3. Afterwards, lentivirus recombinant plasmid si-IRAK3 was constructed for IRAK3 silencing. Next, cardiac function parameters were measured in response to IRAK3 silencing. The regulatory effects that IRAK3 had on myocardial infarct size and the content of myocardial interstitial collagen were analyzed. The regulation of IRAK3 silencing on the NF-κB signaling pathway was further assayed. The obtained results indicated that highly expressed IRAK3 and activated NF-κB signaling pathway were observed in myocardial tissues of mouse models of AMI, accompanied by increased expression of matrix metalloproteinase (MMP)-2/9 and tissue inhibitor of metalloproteinase 2 (TIMP-2). Notably, IRAK3 gene silencing inhibited the activation of NF-κB signaling pathway. Furthermore, IRAK3 gene silencing led to the decreased thickness of infarct area and collagen content of myocardial interstitium, alleviated diastolic, and systolic dysfunctions, as well as, facilitated cardiac functions in mice with AMI, corresponding to decreased expression of MMP-2/9 expression and increased expression of TIMP-2. Taken together, silencing of IRAK3 inactivates the NF-κB signaling pathway, and thereby impeding the cardiac rupture and ventricular remodeling, which eventually prevents AMI progression.     

西南地区野生狗牙根种质资源的SSR与AFLP联合分析

为了制定科学的资源保护策略、促进其开发利用以及新品种的选育,对西南地区55份野生狗牙根材料,联合采用 SSR 和 AFLP 方法分析其遗传多样性.通过进行引物筛选,筛选出有效引物组合18对和11对;SSR 和 AFLP 扩增总条带353和626条,多态性条带267和592条,平均每对引物扩增出多态性条带19．61和53．83条,多态性位点百分率为75．10％和94．57％.聚类分析表明 GS＝0．806时,可将所有供试材料分成6个组群,当 GS＝0．82时,可将第 VI 个组群分成4个小组,当 GS＝0．836时,第 VI 组群中第3小组的14个材料分为3个组群基于 Shannon 多样性指数,类群内的遗传变异占总变异的67．41％,类群间的遗传变异占总变异的32．59％.基于 Nei 氏无偏估计的遗传一致度的聚类分析表明,各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性.通过两种标记的相关性分析,表明 SSR 和 AFLP 标记之间具有显著的相关性,而且相关性较高,证明供试材料的聚类和其生态地理环境间有一定的相关性;采用 SSR 和 AFLP 两种联合分析,结果会更加合理和有效.     

A new species of genus Syntactus Förster (Hymenoptera, Ichneumonidae, Ctenopelmatinae) with a key to Oriental and Eastern Palearctic species

A new species, Syntactus jiulianicus Sun & Sheng, sp.n. belonging to the tribe Pionini of the subfamily Ctenopelmatinae (Hymenoptera, Ichneumonidae), collected from Jiangxi Province, China, is reported. Illustrations of the new species and Syntactus delusor (Linnaeus, 1758), Syntactus minor (Holmgren, 1857) and Syntactus varius (Holmgren, 1858) are provided. A key to the species of Syntactus known from the Oriental and Eastern Palaearctic Regions is given.     

蒺藜苜蓿MtROP5启动子与GUS基因融合构建的稳定转化及其表达特性

本文以3～4周大小的蒺藜苜蓿R108．1的叶片为外植体,通过根癌农杆菌介导的遗传转化系统将MtROP5启动子与GUS报告基因的融合构建导入蒺藜苜蓿中,该转化系统经体胚发生产生转化的再生植株。利用PCR扩增对转化植株进行初步鉴定,并通过GUS4J6学染色法分析MtROP5启动子的组织表达特性。结果表~）]MtROP5启动子主要在蒺藜苜蓿根、花、果荚、叶片和种子中表达。     

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2012 - 31 May 2012

This article documents the addition of 123 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brenthis ino, Cichla orinocensis, Cichla temensis, Epinephelus striatus, Gobio gobio, Liocarcinus depurator, Macrolophus pygmaeus, Monilinia vaccinii-corymbosi, Pelochelys cantorii, Philotrypesis josephi, Romanogobio vladykovi, Takydromus luyeanus and Takydromus viridipunctatus. These loci were cross-tested on the following species: Cichla intermedia, Cichla ocellaris, Cichla pinima, Epinephelus acanthistius, Gobio carpathicus, Gobio obtusirostris, Gobio sp. 1, Gobio volgensis, Macrolophus costalis, Macrolophus melanotoma, Macrolophus pygmaeus, Romanogobio albipinnatus, Romanogobio banaticus, Romanogobio belingi, Romanogobio kesslerii, Romanogobio parvus, Romanogobio pentatrichus, Romanogobio uranoscopus, Takydromus formosanus, Takydromus hsuehshanesis and Takydromus stejnegeri.     

茂原链霉菌谷氨酰胺转胺酶序列分析及三维结构建模

利用DNASTAR、VectorNTI9、SignalP等生物信息学平台对茂原链霉菌(Streptomyces mobaraensis)谷氨酰胺转胺酶(TGase)进行了序列分析和三维结构建模。结果表明:茂原链霉菌TGase同已报道其他微生物来源的TGase有较高的同源性,无信号肽,存在一个跨膜结构区,二级结构是由多个α螺旋、转角和少量β折叠构成。同时在一二级结构分析的基础上,利用同源建模的方法完成了三维结构的建模。