排序方式: 共有229条查询结果,搜索用时 15 毫秒
51.
Lai SC Chong PC Yeh CT Liu LS Jan JT Chi HY Liu HW Chen A Wang YC 《Journal of biomedical science》2005,12(5):711-727
Summary The spike (S) glycoprotein is thought to play a complex and central role in the biology and pathogenesis of SARS coronavirus
infection. In this study, a recombinant protein (rS268, corresponding to residues 268–1255 of SARS-CoV S protein) was expressed
in Escherichia coli and was purified to near homogeneity. After immunization with rS268, S protein-specific BALB/c antisera and mAbs were induced
and confirmed using ELISA, Western blot and IFA. Several BALB/c mAbs were found to be effectively to neutralize the infection
of Vero E6 cells by SARS-CoV in a dose-dependent manner. Systematic epitope mapping showed that all these neutralizing mAbs
recognized a 15-residues peptide (CB-119) corresponding to residues 1143–1157 (SPDVDLGDISGINAS) that was located to the second
heptad repeat (HR2) region of the SARS-CoV spike protein. The peptide CB-119 could specifically inhibit the interaction of
neutralizing mAbs and spike protein in a dose-dependent manner. Further, neutralizing mAbs, but not control mAbs, could specifically
interact with CB-119 in a dose-dependent manner. Results implicated that the second heptad repeat region of spike protein
could be a good target for vaccine development against SARS-CoV. 相似文献
52.
Identification of single-chain antibody fragments specific against SARS-associated coronavirus from phage-displayed antibody library 总被引:4,自引:0,他引:4
Liu ZX Yi GH Qi YP Liu YL Yan JP Qian J Du EQ Ling WF 《Biochemical and biophysical research communications》2005,329(2):437-444
To develop early diagnostic reagents, effective vaccines, and even drugs against SARS-associated coronavirus (SARS-CoV), the human single fold single-chain antibody fragments, (scFv) libraries I+J (Tomlinson I+J) were used to identify novel scFvs, which can specifically bind to SARS-CoV. Interestingly, two scFvs (B5 and B9) exhibited higher binding specificity to SARS-CoV with the OD(450) value 0.608 and 0.545, respectively, and their coding sequences shared the identical sequence composed of V(H) gene (351bp) and V(L) gene (327bp), so the two scFvs were uniformly named as SA59B and chosen for further analysis. SA59B scFv was expressed in soluble form in Escherichia coli HB2151 and purified by immobilized metal affinity chromatography. The soluble 30kDa SA59B scFv-antibody was verified in SDS-PAGE and Western-blot. The purified SA59B scFv-antibody was labeled with HRP by the glutaraldehyde method, and the concentration of HRP and SA59B scFv-antibody in the SA59B-HRP solution reached 2.4 and 2.28mg/ml, respectively. Then, the binding ability of SA59B-HRP to SARS-CoV was evaluated by ELISA with S/N of 11.6, indicating higher binding specificity between them. Finally, both the SA59B sequence specificity and its application for diagnosis, prophylaxis or therapy of SARS were discussed. 相似文献
53.
Lu JH Zhang DM Wang GL Guo ZM Li J Tan BY Ou-Yang LP Ling WH Yu XB Zhong NS 《Acta biochimica et biophysica Sinica》2005,37(7):473-479
The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5. In this study, bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5. Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP). The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins. The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study provided the foundation for further exploration of the protein‘s biological function, and contributed to the search for anti-SARS-CoV drugs. 相似文献
54.
Nighat Perveen Sabir Bin Muzaffar Mohammad Ali Al-Deeb 《Saudi Journal of Biological Sciences》2021,28(2):1417-1425
The novel coronavirus disease (COVID-19) that emerged in December 2019 had caused substantial morbidity and mortality at the global level within few months. It affected economies, stopped travel, and isolated individuals and populations around the world. Wildlife, especially bats, serve as reservoirs of coronaviruses from which the variant Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) emerged that causes COVID-19. In this review, we describe the current knowledge on COVID-19 and the significance of wildlife hosts in its emergence. Mammalian and avian coronaviruses have diverse host ranges with distinct lineages of coronaviruses. Recombination and reassortments occur more frequently in mixed-animal markets where diverse viral genotypes intermingle. Human coronaviruses have evolved through gene gains and losses primarily in interfaces where wildlife and humans come in frequent contact. There is a gap in our understanding of bats as reservoirs of coronaviruses and there is a misconception that bats periodically transmit coronaviruses to humans. Future research should investigate bat viral diversity and loads at interfaces between humans and bats. Furthermore, there is an urgent need to evaluate viral strains circulating in mixed animal markets, where the coronaviruses circulated before becoming adapted to humans. We propose and discuss a management intervention plan for COVID-19 and raise questions on the suitability of current containment plans. We anticipate that more virulent coronaviruses could emerge unless proper measures are taken to limit interactions between diverse wildlife and humans in wild animal markets. 相似文献
55.
Yu-xuan HOU Cheng PENG Zheng-gang HAN Peng ZHOU Ji-guo CHEN Zheng-li SHI 《Virologica Sinica》2010,(1)
A group of SARS-like coronaviruses(SL-CoV)have been identified in horseshoe bats.Despite SL-CoVs and SARS-CoV share identical genome structure and high-level sequence similarity,SL-CoV does not bind to the same cellular receptor as for SARS-CoV and the N-terminus of the S proteins only share 64%amino acid identity,suggesting there are fundamental differences between these two groups of coronaviruses.To gain insight into the basis of this difference,we established a recombinant adenovirus system expressing t... 相似文献
56.
Amarnath Chatterjee Margaret A. Johnson Pedro Serrano Bill Pedrini Kurt Wüthrich 《Biomolecular NMR assignments》2007,1(2):191-194
Sequence-specific NMR assignments of an internal domain of the protein nsp3, nsp3(513–651), which is a part of the SARS coronavirus
(SARS-CoV) replicase polyprotein, have been determined, using triple-resonance NMR experiments with the uniformly [13C,15N]-labeled protein. The complete assignments (>99%) provide the basis for the ongoing three-dimensional structure determination. 相似文献
57.
《Journal of molecular biology》2021,433(10):166945
The COVID-19 pandemic entered its third and most intense to date wave of infections in November 2020. This perspective article describes how combination therapies (polytherapeutics) are a needed focus for helping battle the severity of complications from SARS-CoV-2 infection. It outlines the types of systems that are needed for fast and efficient combinatorial assessment of therapeutic candidates. Proposed are micro-physiological systems using human iPSC as a format for tissue-specific modeling of infection, the use of gene-humanized zebrafish and C. elegans for combinatorial drug screens due to the animals being addressable in liquid multi-well formats, and the use of engineered pseudo-typing systems to safely model infection in the transgenic animals and engineered tissue systems. 相似文献
58.
Lauren B. Rodda Jason Netland Laila Shehata Kurt B. Pruner Peter A. Morawski Christopher D. Thouvenel Kennidy K. Takehara Julie Eggenberger Emily A. Hemann Hayley R. Waterman Mitchell L. Fahning Yu Chen Malika Hale Jennifer Rathe Caleb Stokes Samuel Wrenn Brooke Fiala Lauren Carter Marion Pepper 《Cell》2021,184(1):169-183.e17
59.
Daniel Asarnow Bei Wang Wen-Hsin Lee Yuanyu Hu Ching-Wen Huang Bryan Faust Patricia Miang Lon Ng Eve Zi Xian Ngoh Markus Bohn David Bulkley Andrés Pizzorno Beatrice Ary Hwee Ching Tan Chia Yin Lee Rabiatul Adawiyah Minhat Olivier Terrier Mun Kuen Soh Frannie Jiuyi Teo Cheng-I Wang 《Cell》2021,184(12):3192-3204.e16
60.
Severe Acute Respiratory Syndrome (SARS) is a deadly infectious disease caused by SARS Coronavirus (SARS-CoV). Inactivated
SARS-CoV has been explored as a vaccine against SARS-CoV. However, safe and potent adjuvants, especially with more efficient
and economical needle-free vaccination are always needed more urgently in a pandemic. The development of a safe and effective
mucosal adjuvant and vaccine for prevention of emergent infectious diseases such as SARS will be an important advancement.
PIKA, a stabilized derivative of Poly (I:C), was previously reported to be safe and potent as adjuvant in mouse models. In
the present study, we demonstrated that the intraperitoneal and intranasal co-administration of inactivated SARS-CoV vaccine
together with this improved Poly (I:C) derivative induced strong anti-SARS-CoV mucosal and systemic humoral immune responses
with neutralizing activity against pseudotyped virus. Although intraperitoneal immunization of inactivated SARS-CoV vaccine
alone could induce a certain level of neutralizing activity in serum as well as in mucosal sites, co-administration of inactivated
SARS-CoV vaccine with PIKA as adjuvant could induce a much higher neutralizing activity. When intranasal immunization was
used, PIKA was obligatorily for inducing neutralizing activity in serum as well as in mucosal sites and was correlated with
both mucosal IgA and mucosal IgG response. Overall, PIKA could be a good mucosal adjuvant candidate for inactivated SARS-CoV
vaccine for use in possible future pandemic. 相似文献