中空纤维细胞培养和传代细胞应用的浅析

中空纤维细胞培养和传代细胞应用的浅析王代荣（卫生部成都生物制品研究所，成都６１００６３）中空纤维细胞和传代细胞都可用来制备病毒抗原。都是一种比较新颖的培养技术。一、中空纤维细胞培养中空纤维细胞培养技术是１９７２年以后出现的一种新的培养技术。目前使用的...     

pSV—2neo和HPV—Ⅱ DNA共同转染NIH3T3细胞的结果

本实验将neo及HPV-11两种DNA共同转染NIH 3T3细胞,以G418抗生素作为选择剂,对诱导的转化灶进行筛选。同时还就G418对NIH 3T3细胞的毒性进行了观察。neo单独使用诱导的转化灶数为44.00/1×10~5;neo与HPV-11合用诱导的转化灶数为162.66/1×10~5。由neo转化的细胞含有neo基因,由neo和HPV-11转化的细胞内含有该两种基因。     

恒河猴脾肾细胞中的ANP样物质

用兔抗人α-心房利钠多肽(atrial natriure-tic peptide,ANP)血清和PAP免疫组织化学方法,观察到恒河猴脾白髓和肾髓质上皮细胞中存在ANP样免疫活性物质,其棕色颗粒,主要分布于细胞核周质内。猴脾提取物经Seph-adex G-15分离,可得到具有降压、利尿、利钠的组分,这些活性多肽的结构和功能需待进一步研究。     

MCF—7细胞在亚多层中细胞膜极性的变化

Sub-multilayer of MCF-7 cell, an established human breast carcinoma cell line, was achieved by culturing the cells on millipore filters for a long time. The superficial layer cells maintained their membrane polarity features as MCF-7 cells in monolayers did. MAM-6, a human milk fat globule membrane antigen, was polarized distributed in apical domain of 97.5% superficial layer cells revealed by immunoperoxidase cytochemistry. Whereas, among the low layer cells, which had no free surface (apical domain) toward the culture medium and did not show morphological polarity features, only 12.9% expressed surface MAM-6 with weak immunoperoxidase staining and random distribution. But the immunostaining for detecting cytoplasmic MAM-6 in low layer cells was stronger than that in superficial layer cells, indicating that the vectorial delivery and insert of MAM-6 carrying glycoprotein to the plasma membrane seemed to be stopped or declined and became undirectional in the later situation. The study demonstrates that an asymmetric spatial environment, which is composed of a liquid phase space and a solid phase space, is crucial for the establishment of epithelial membrane polarity of MCF-7 cells.     

大肠杆菌表达的重组细胞色素P—450nor的分离纯化

用ＤＥ－５２纤维素柱色谱法和ＦＰＬＣ法分离纯化了大肠杆菌表达的重组缣孢菌色素Ｐ－４５０ｎｏｒ，经梯度洗脱ＭｏｎｏＱ纯化后的ｆＲ．Ｐ－４５０ｎｏｒ为单一色谱峰，比活达５５．２０Ｕ／ｍｇ、纯化倍数约为１１００倍，ＳＤＳ－ＰＡＧＥ检测为单一谱带。     

国外信息

1.细胞蛋白可通过表观遗传调控使反转录病毒沉默:发现一种新的抗病毒作用(J Virol,2008,82:2313～2323).     

一种非破坏性监测培养植物细胞的简易方法

在研究悬浮培养中植物细胞的生长规律时,常常采用多次分批法测定生长曲线,但工作量大,且存在着个体差异,以致测定的结果有误差。为此,我们研制出一体化非破坏性监测的培养装置(图1),对剌五加体细胞胚进行培养,监测其体积增长变化,并得到剌五加体细胞胚的生长模型,现简介如下。 1 培养装置由培养的三角瓶和监测体积的量筒组成,两者的容积比为5∶2。结构简单,操作方便。