类器官的构建与应用进展

类器官是在体外经由干细胞驱动的, 形成具有来源器官显微解剖特征的多细胞三维结构且能自我更新的微组织。类器官能分化产生器官特异性的多种细胞类型,能重现对应器官的部分功能和空间架构,它的诞生为生命医学研究和临床应用注入了新动能,在癌症基础与临床研究、再生医学等领域表现出广阔的应用前景。对近些年国内外类器官研究进展进行综述,介绍其构建过程与培养体系,并详细阐述其作为体外研究模型的优缺点,为基于类器官的科学研究与应用提供了参考。     

Influence of operation conditions and additives on the development of producer gas and tar reduction in air gasification of construction woody wastes using a two-stage gasifier

Air gasification was conducted with fractions of construction woody wastes in a two-stage gasifier, consisting of a fluidized bed zone and a tar cracking zone. The aim of this work is to investigate the influence of reaction conditions and additives on the composition of producer gas and tar content in producer gas.A producer gas obtained with activated carbon of 540 g at an ER of 0.26 was mainly composed of H₂ (25 vol.%), CO (22 vol.%) and CH₄ (5 vol.%). Regarding tar removal efficiency, activated carbon was better than olivine. The tar removal rate with virgin activated carbon reached up to 80%. The reuse of spent activated carbon caused an efficiency loss in tar removal to some extent. Overall, it seems that the strong need for intensive downstream tar removal measurements can be removed with the use of a two-stage gasifier and the application of activated carbon.     

cDNA文库的构建策略及其应用

cDNA文库在基因分离和克隆中具有重要的作用。从cDNA文库中能筛选出所需要的目的基因,并直接用于该目的基因的表达。cDNA文库是发现新基因和研究基因功能的基础工具。随着分子生物学技术的发展。cDNA文库构建方法有了很大改进和提高,就cDNA文库的构建方法及其应用进行综述。     

从灾害救援特点看新时期心理卫生支援分队建设

根据自然灾害医学救援要求及组织体制,结合灾害救援过程中的心理压力来源及心理需求,提出心理卫生支援分队建设指导:人员需具备专业技能,兼顾全面技术;采取的工作方式主要为团体合作、小组协作和单人干预等;日常训练重点在于做好实战训练、预案、心理和物资方面的准备,力求使心理卫生支援分队满足新时期非战争军事任务的需要。     

生物工程设备精品课程建设实践与探索

生物工程设备是现代工业生物技术转化为现实生产力和发挥作用的关键环节,随着工业生物技术的迅速发展,对掌握现代生物工程设备原理、设计和操作的人才的需求日益增加.本文分别从师资队伍建设、教材建设、教学内容及教学体系优化、教学方法革新等方面对生物工程设备精品课程建设经验进行了总结.     

生物能源产业生态系统的构建及其特征研究

生物能源产业在各国政府的大力扶持下得到迅速发展,但与此同时,伴随发展生物能源产业而来的经济、社会和环境等问题也不断浮出水面。本文对“生物能源产业生态系统”的系统框架进行构建,阐述“生物能源产业生态系统”的基本内涵,解析系统的组织形式及其边界,分析系统的构成,并进一步总结了系统所具有的主要特征,探讨系统所具有的各项功能。     

siRNA表达载体的构建及其表达

本研究用限制性内切酶消化质粒pCMV-tag-2B,除去巨细胞病毒(Cytomegalovirus,CMV)启动子核苷酸序列,剩下的核苷酸序列作为构建表达siRNA(Small interfering RNA,siRNA)载体的前体。依据文献提供的扩增H1RNA启动子核苷酸序列的引物序列合成一对引物,以带有H1RNA启动子序列的质粒DNA为模板扩增HIRNA启动子序列,插入前体,构建SiRNA的表达载体pCH1。另外将H1RNA启动子插入pGEM．1lfz相应位点,构建瞬时表达载体pGHl。依据EGFP的有效SiRNA抑制位点,合成两条分别为64bp的核苷酸链,通过体外退火,形成双链,然后插入已构建的两个表达载体。将这两个载体分别与表达EGFP蛋白的质粒pEGFP．N3共转染Bel．7402细胞,观察siRNA对EGFP的抑制效应。研究结果表明构建的载体有效表达了siRNA,这些载体可以用于与siRNA相关抗病毒治疗性试验研究。     

环境工程微生物学综合性实验教学体系的构建与实践

环境工程微生物学实验是环境工程专业一门重要的专业基础必修课。该课程对专业课的学习具有重要影响。为了保证该课程的教学质量,我们构建了一个新的实验教学体系。该体系包括两部分内容:一是絮凝细菌的分离、筛选、条件优化、应用效果及特征分析;二是污染控制工程中放线菌、霉菌、酵母菌、原生动物及微型后生动物种类与数量分布调查。两部分实验均具有研究性和综合性。第一部分内容分为5个模块。这5个模块具有系统性和连续性,且有一定的挑战性。通过这部分实验,学生可掌握有关细菌的基本实验技术及研究方法。第二部分内容所涉及的是丝状微生物和真核微生物。这部分实验可使学生掌握此类微生物的基本实验技术及其应用方法。该体系内容丰富,与专业结合紧密,大大激发了学生的学习热情。通过该体系,可使学生全面获得环境工程微生物基本实验技能,并有助于提高学生的综合能力,教学效果显著。     

节肢动物α-淀粉酶系统进化树构建及分析

目的:构建节肢动物α-淀粉酶的系统进化树,探讨其进化关系,找出进化树中聚类在一起的α-淀粉酶的特异性序列。方法:在美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库中选取了56个节肢动物的α-淀粉酶氨基酸序列,利用CLUSTALX2.0进行序列比对、MEGA6.0建立进化树,通过BOXSHADE找到聚类的α-淀粉酶特异性序列。结果:56个α-淀粉酶聚类成A、B、C、D四大簇,A簇特异性序列为"VD NHD NQ",B簇特异性序列为"ID NHD NX",C簇特异性序列为"ID NHD NQ",D簇特异性序列为"XGN NHD X"。A、B、C、D四簇都含有保守的NHD(天冬酰胺-组氨酸-天冬氨酸)序列,但序列两端氨基酸种类不同。结论:56个节肢动物α-淀粉酶分为4簇,每簇都有其特异性序列,但都含有保守序列NHD。     

Urban ecological corridors construction: A review

An ecological corridor, with both ecological and cultural functions, is a symbol of urban ecological or green civilization, and has therefore become one of the major topics in the fields of landscape ecology, urban ecology, and ecological planning. On the one hand, along with the prominent contradiction between regional ecological protection and economic development, as well as between the growing ecological demands of urban residents and the destruction of natural ecosystems, the construction of urban ecological corridors is very challenging. On the other hand, with contemporary urbanization and ecological civilization development, the standards and requirements for the construction of urban ecological corridors are set higher and higher. Constructing an urban ecological corridor is therefore particularly important, and must adopt a spatial approach that balances the relationship between ecological protection and economic development. In this study, the classification of urban ecological corridors was firstly conducted according to the structural or functional differences. Secondly, research progress on the construction of urban ecological corridors was systematically summarized and the main inadequacies were indicated. Following the analysis of the main methods employed in the construction of urban ecological corridors, existing methods were classified into three kinds, i.e. qualitative analysis, quantitative analysis, and spatial analysis. In addition, the advantages and disadvantages of the methods of subjective judgment, suitability and sensitivity analysis, network analysis, and minimum cost path analysis were compared. To provide theoretical support for the construction and management of urban ecological corridors, four key research directions were also pointed out, i.e. the identification of key nodes of urban ecological corridor, the determination of the width of urban ecological corridor, the measurement of integrated effect of urban ecological corridor, and the multi-scale integration of urban ecological corridor. The present study will aid in accelerating and improving the process of ecological corridors construction in China's new-type urbanization.