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为探讨雄激素对人前列腺中鸟氨酸脱羧酶( O D C)基因表达的调节作用,以研究雄激素诱导前列腺良性增生的分子机理,分离培养了人胎儿前列腺间质细胞,以 M T T 法测定不同浓度 D H T对细胞的促增殖作用;以最适浓度的 D H T(1 000 μg/ L)刺激该细胞,分别于 0,3,6,12,24,30 h 提取总 R N A,用斑点杂交及 Northern blot 法分析测定各组细胞中 O D C m R N A 的丰度,并对杂交膜进行薄层扫描定量.结果显示:(1) D H T 对前列腺间质细胞的增殖呈双相调节作用,即在低浓度时随着 D H T 浓度的增加,对该细胞的促增殖作用增强,1 000 μg/ L时刺激活性最强,高浓度 D H T 对该细胞的刺激作用降低.(2)斑点杂交显示,在 1 000 μg/ L D H T 刺激细胞后 6 h 时, O D C m R N A开始明显升高,24 h 达高峰(约为 0 h 的 48 倍),至 30 h 有所降低.(3) Northern blot 结果显示,人胎儿前列腺间质细胞中有两种 O D C m R N A,分别为 20 kb 和 26 kb,经扫描定量结果显示:1 000μg/ L D H T 对两种 O D C m R N A 相似文献
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新生儿PON2基因多态性与早产的关系 总被引:4,自引:0,他引:4
探讨新生儿对氧磷酶2基因多态性(PON2148,PON2311)对早产的影响。采用横断面调查方法,使用统一的调查表,由安庆市各县医院对入院分娩孕妇及其单胎、活产、早产和对照新生儿进行调查,共得到有效样本194个母亲-新生儿对。单因素分析结果显示:PON2 Ala148Ala纯合子基因型与Gly148Gly纯合子基因型 / Ala148Gly杂合子基因型比较致早产的危险性升高且有显著意义;同样,PON2 Ser311Ser纯合子基因型致早产的危险性升高且有显著意义。进一步分析PON2148位点多态性和PON2311位点多态性是否存在交互作用,结果显示:这两个位点多态性之间无明显交互作用。对氧磷酶2基因PON2148位点多态性和PON2311位点多态性与新生儿早产相关,但PON2148位点多态性和PON2311位点多态性之间对早产的影响无明显交互作用。
Association of PON2 Gene Polymorphisms in Neonates with Preterm
LIANG Hong-ye1,WU Bai-yang1,CHEN Da-fang1,YANG Fan2,HU Hai-yan2,CHEN Li1,XU Xi-ping1.
1.Department of Biology & Genetics,Peking University Health Science Center,Beijing 100083,China;
2.Anqing Branch of Institute for Biomedicine,Anhui Medical University,Anqing 246000,China
Abstract:The objective is to investigate whether gene polymorphisms in the PON2 gene (PON2148 and PON2311) of neonates are associated with preterm. Using standard questionnaires,194 singleton live born mother-neonate pairs (include preterm cases and term controls) were investigated by the trained field workers with cross-sectional survey at the hospitals in Anqing,Anhui Province,China. Epidemiological and clinical data and blood samples were obtained from 194 mother-neonate pairs. Among neonates,PON2 Ala148Ala homozygote is significantly associated with preterm,compared with Gly148Gly homozygote / Ala148Gly heterozygote before and after adjustment confounders and the same was true for PON2 Ser311Ser homozygote. However,when PON2148 polymorphism and PON2311 polymorphism were considered jointly,no significant gene interaction between PON2148 polymorphism and PON2311 polymorphism in relation to preterm was observed. We draw a conclusion from this research that both PON2148 polymorphism and PON2311 polymorphism in neonates are significantly associated with preterm respectively. But the gene interactions between PON2148 polymorphism and PON2311 polymorphism in neonates are not significantly associated with preterm.
Key words:paraoxonase 2 gene (PON2 gene);gene polymorphism;preterm;genotype 相似文献
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HPV16重组减毒沙门氏菌表达质粒的构建及其诱导免疫 总被引:1,自引:0,他引:1
将人乳头瘤病毒(Human papillomavirus,HPV)16 L1E7基因插入携带霍乱毒素基因的pET-CTA2B载体,获得HPV16L1E7与CTA2B融合表达质粒pET-L1E7CTA2B.在此基础上,通过PCR基因克隆技术,将pET载体的T7启动子与pTETnir15载体的大肠杆菌硝酸盐还原酶基因B启动子nirB进行分割重组,将nirB启动子的厌氧调控元件和启动子基本元件FNR-TATA盒与T7启动子的核糖体结合位点(SD)序列融合,形成nirB-T7杂合启动子.最后获得HPV16重组减毒沙门氏细菌疫苗表达载体pNir-16L1E7CTA2B,并进一步构建对照质粒pNir-16L1E7.Western blot结果证实以上质粒nirB-T7杂合启动子能够在沙门氏细菌中表达L1E7融合蛋白.口服免疫接种小鼠可成功诱导小鼠生殖道产生HPV16特异性粘膜免疫,且霍乱毒素CTA2B可以增强粘膜免疫诱导能力,为开发廉价有效的HPV16预防性疫苗打下了基础. 相似文献