龟板炮制前后氨基酸的分析

<正> 龟板系龟科动物鸟龟(Chinemgs ree-vsii)的腹甲,为传统滋补中药,具有滋阴潜阳,益肾强骨,养心补血的功效,主要有效成分为多种氨基酸。龟板一般按药典规定的方法经炮制后作为药用。有关氨基酸的系统分析,国内外尚未见报导。为了探讨龟板的内在质量、同时比较其炮制前后氨基酸含量的变化,我们对龟板中所含游离氨基酸及其水解后的氨基酸进行了分析研究。分析结果表明,炮制前的样品中含有I7种游离氨基酸,炮制后则只含有15种游离氨基酸,且前者含量明显高于后者;龟板炮制前后的样品经盐酸水鲜后均测得17种氨基酸,且前者含     

猪肺炎支原体膜上ATP酶生化性质的研究

猪肺炎支原体膜上ATP酶为Mg~(2+)激活,乌巴因不抑制。DCCD和寡霉素对该酶也无抑制作用,只有NBD与Quercetin才有一定的抑制效果。用梯度凝胶电泳可获均一的具有活性的酶蛋白带。     

尾壳核内注射脑啡肽和bestatin对大鼠操作式条件...

Female Wistar rats were trained in a Skinner-box, 30 trials per day in a dark room to establish operant defence conditioning. Training started with a light (15 s), then combined with footshock for further 8 s. When the rats learned to press the key to avoid footshock within 15 s, conditioned response was considered established. After the rats reached a conditioning rate (CR) above 80% for 5 days, cannulae were implanted into caudate-putamen. Two to three days later, Met-enkephalin (MEK) or bestatin (an aminopeptidase inhibitor) was injected bilaterally into caudate-putamen. 30 min, 2 h, 24 h and 48 h after injection, conditioning tests were conducted, with each session consisting of 30 trials. Control experiments were done when 0.9% NaCl (NS) was injected. After injection of NS, CR maintained above 80% in all 4 test sessions. MEK (60 ng/rat) or bestatin (10 micrograms/rat) significantly lowered the CR during the 30 min and 2 h test session. In the latter case, the latency (L) was also prolonged. However both CR and L returned to the control level in the 24 h and 48 h test sessions. Naloxone (2 mg/kg, i.p.) blocked the conditioning-depression effect of bestatin. No significant alteration was seen in locomotor activity after MEK or bestatin injection. The results suggest that enkephalin in caudate-putamen may be involved in the regulation of retrieval of conditioning. Bestatin mimics the effect of MEK on conditioning reflex probably by increasing production of endogenous enkephalin.     

HPLC法定量分析植物组织中ABA,IAA和NAA

近年来有关植物激素的提取、分离方法的报道时有出现,但大都是沿用80％甲醇(MeOH)从植物组织中提取IAA和ABA,用乙酸乙酯萃取酸化的提取液后得到粗提液,粗提液再经过纤维素薄层层析、硅胶层析、     

中国农业害虫生物防治概况与进展

<正> 随着我国经济建设的不断发展和社会对生态环境意识的增强,生物防治科技工作在政府大力支持和广大科技人员共同努力下,得到迅速发展。“七五”和“八五”期间经政府部门批准正式列入国家科技攻关计划,并由中国农科院生防所主持,组织全国30多个科研和教学单位4百多位科技人员进行协作攻关。与此同