排序方式: 共有20条查询结果,搜索用时 14 毫秒
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用花药愈伤组织作为转化受体的水稻转基因植株的分析 总被引:1,自引:0,他引:1
以花药愈伤组织作为转化的受体材料,利用农杆菌介导法将已克隆的Xa21基因导入粳稻栽培品种台北309中。共获得7个转基因株系,其中2个单倍体,4个二倍体,1个混倍体。PCR、Southern、FISH以及白叶枯病抗性的分析结果都表明.Xa21基因已整合到T0代受体基因组。调查了4个二倍体株系T1代的分离情况,经x^2测验证明,有2个株系的分离比为3:1,为单拷贝插入,另外2个株系不符合孟德尔分离。4个T0代二倍体转基因株系应为杂合二倍体。 相似文献
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一种紫色水稻的遗传及其在光敏不育系育种中应用的研究 总被引:15,自引:0,他引:15
潘学彪 陈宗祥 董桂春 汤述翥 李欣 于恒秀 程祝宽PAN Xue-Biao CHEN Zong-Xiang DONG Gui-Chun TANG Shu-Zhu LI Xin YU Heng-Xiu CHENG Zhu-Kuan 《遗传》1995,17(3):31-34
研究表明,本院获得的一种紫色水稻的植株色遗传受控于C、 A、Pl3个独立基因座位上显性基因的互补作用,另有一独立的显性基因对Pl基因的表达起抑制作用。由于该抑制基因在籼稻中的高频率存在,因而,紫稻与一般绿稻品种杂交F1多表现为绿株。紫稻光敏不育(株)系的不育性表达和配合力表现,均可达到与普通光敏不育系相似的水平。本文还讨论了选育籼型紫稻光敏不育系设想的可行性。
Abstract:The inheritance of a purple rice in crosses to green rices was investigated.A group of dominant and interactive genes,C,A and Pl,was found to control the expression of the trait and the other independent inhibitor I-Pl-1 to inhibit the effect of the gene Pl.Because of the wide existence of the gene I-Pl-1 in indica rice,most of F1 plants of the crosses between purple rice and green cultivars were green.The primary study indicated that,for the purple photoperiod-sensitive genic male-sterile lines,the degrees of the sterility and its stability in the sterile stage,and of the fertility in the fertile stage,and the combining ability levels of the purple rice were as high as the degrees and levels for the green rice.A tentative idea on breeding purple photoperiod-sensitive male-sterile lines of indica rice was suggested and its feasibility and advantage was discussed. 相似文献
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水稻染色体形体较小, 很难从形态上区分各别染色体。尽管有许多学者报道了水稻核型的研究结果, 但不同学者之间的分歧依然存在。本文对不同学者所作核型进行了分析, 从而对现行的水稻染色体编号系统提出了新的看法。
Abstract As rice chromosome is very small, it is difficult to identify morphologically the individual chromosome from rice chromosome complement. Although many studies have been conducted on the karyotype of rice, controversies still exist among different researchers. The present paper summerized the data of rice karyotype given by different researchers, and some suggestions were also proposed on rice chromosome numbering system. 相似文献
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遗传异常分离既是自然界非常普遍的现象, 也是生物进化的动力之一。产生异常分离的原因可能与配子体或孢子体的选择有关。利用6个以类病变(lmi)和矮杆突变体(d6)为亲本的杂交组合(F2或F3), 对该类病变和矮杆基因的遗传规律及异常分离现象作初步的分析。结果显示, lmi×02428和d6×93-11的F2群体以及F3株系中存在极端异常分离的现象; LMI基因附近的分子标记ST8-1和D6基因附近的ST7-1、ST7-2、RM5490的带型分离同样也极显著偏离期望比; 偏分离因子与类病斑LMI和矮杆基因D6紧密连锁, 分别位于第8染色体分子标记ST8和ST8-2之间以及第7染色体分子标记ST7-1和ST7-3之间。异常分离现象还与杂交的组合有直接的关系。 相似文献