CDC42 抑制剂ML141对喉癌Hep-2 细胞增殖的抑制作用

目的:探讨CDC42抑制剂ML141对喉癌细胞增殖的抑制作用,为喉癌的分子治疗提供新的靶点。方法:体外培养人喉癌Hep-2细胞。实时定量聚合酶链反应(Real-time PCR)检测CDC42在Hep-2细胞中的表达。利用GLISA法检测ML141对CDC42活性的抑制效果。利用CCK8法检测ML141对Hep-2细胞增殖能力的抑制效果。结果:1Real-time PCR结果显示在人喉癌Hep-2细胞中CDC42显著高表达(P0.001),证明全基因组的结果准确。2GLISA结果显示表皮生长因子作用的Hep-2细胞中CDC42的活性明显高表达,但加入ML141的Hep-2细胞中CDC42的活性受到明显抑制。(P0.001)。3CCK8结果显示24 h,48h和72 h时,ML141处理的Hep-2细胞的增殖能力与对照组相比均受到明显抑制。(P0.001)。结论:促癌基因CDC42抑制剂ML141能够抑制人喉癌Hep-2细胞增殖,具有成为抗喉癌新药的潜力,为喉癌的分子治疗提供新的切入点。     

Synthesis and characterization of novel Na15(SO4)5F4Cl:Ce3+ halosulfate phosphors

A series of Na₁₅(SO₄)₅F₄Cl phosphors doped with Ce³⁺ ions was prepared using the wet chemical method. X‐Ray diffraction studies were used to determine their phase formation and purity. Fourier transform infrared spectroscopy effectively identified the chemical bonds present in the molecule. The photoluminescence properties of the as‐prepared phosphors were investigated and the Ce³⁺ ions in these hosts were found to give broadband emission in the UV range. For the thermoluminescence study, phosphors were irradiated with a 5 Gy dose of γ‐rays from a ⁶⁰Co source. Chen’s half‐width method was employed to calculate the trapping parameters from the thermoluminescence glow curve. Copyright © 2016 John Wiley & Sons, Ltd.     

^60Coγ辐射对亚洲玉米螟体内保护酶活力的影响

作研究了亚洲玉米螟Ostrinia furnacalis(Guen．)不同虫态(3～4龄幼虫、1～2d龄蛹、当天羽化成虫)体内过氧化物醇(POD)和过氧化氢醇(CAT)活力在不同^60Coγ辐照条件下的变化。结果表明：亚洲玉米螟不同虫杰的POD活力顺序为;雌成虫>1～2d蛹>雄成虫>3～4龄幼虫;CAT活力为：3～4龄幼虫>雄成虫>雌成虫>1～2d龄蛹。辐照处理后,POD活力随辐照剂量的增加而呈上升趋势,三种虫态均以40Krad处理后曲活力最高;CAT的话力变化较大．3～4龄幼虫、1～2d龄蛹和雄威虫都以30Krad处理曲活力最高,雌戚虫以20Krad处理的最高。本试验结果有助于阐明辐射对昆虫生理生化的效应。     

Isolation of ScFv antibodies of rP27~(Kip1) from phage display libraries constructed from immunized and non-immunized repertoires

Through mRNA extract, RT and a series of PCR, phage antibody libraries were constructed from rP27~(Kipl)immunized and non-immunized mice. After only one round of selection with rP27~(Kipl), clones from each library were chosen randomly and digested by Taq I and Hinf I. 11 of 64 clones from the immunized animal had consistent restriction pattern, while none of the 64 clones from the non-immunized animal had, except that one had the same fragments pattern as that of the 11 clones. The 12 fragments were expressed in E. coli BL21(DE3)/pET-28b( ) system. ELISA showed that some of the fragments could bind to rP27~(Kipl) specifically. All these results implied that specific antibody can be obtained by genetic engineering without hybridoma or many rounds of growth and panning selection.     

脲酶-硝化抑制剂对减缓尿素转化产物氧化及淋溶的作用

利用原状土柱模拟试验,研究了脲酶抑制剂氢醌(HQ),硝化抑制剂包被碳化钙(ECC)和双氰胺(DCD)以及它们的不同组合对尿素转化产物土壤持留、氧化以及淋溶的影响.结果表明,与其它抑制剂处理相比,HQ+DCD组合能有效抑制尿素水解产物的氧化,使其以交换态NH₄⁺的形式在土壤中长时间持留;氧化作用的抑制不仅减少了氧化产物NO₃^-的累积,也降低了NO₃^-淋溶潜势,使其淋入下层土壤的深度仅限在5～10 cm范围内,且淋溶量显著降低.     

苏南太湖水系农业非点源氮污染及其控制对策研究

控制农业非点源对水体的污染是实现区域水质管理的必要组成部分。为此,设计了一系列水田、旱地田间试验和室内模拟实验。试验结果表明,渗漏水硝态氮污染与氮肥施用量呈正相关,同时通过统计处理获得农业非点源氮素污染负荷量,并提出了农业非点源氮素污染的几种有效的控制对策。     

A Single-Domain Llama Antibody Potently Inhibits the Enzymatic Activity of Botulinum Neurotoxin by Binding to the Non-Catalytic α-Exosite Binding Region

Ingestion or inhalation of botulinum neurotoxin (BoNT) results in botulism, a severe and frequently fatal disease. Current treatments rely on antitoxins, which, while effective, cannot reverse symptoms once BoNT has entered the neuron. For treatments that can reverse intoxication, interest has focused on developing inhibitors of the enzymatic BoNT light chain (BoNT Lc). Such inhibitors typically mimic substrate and bind in or around the substrate cleavage pocket. To explore the full range of binding sites for serotype A light chain (BoNT/A Lc) inhibitors, we created a library of non-immune llama single-domain VHH (camelid heavy-chain variable region derived from heavy-chain-only antibody) antibodies displayed on the surface of the yeast Saccharomyces cerevisiae. Library selection on BoNT/A Lc yielded 15 yeast-displayed VHH with equilibrium dissociation constants (K_d) from 230 to 0.03 nM measured by flow cytometry. Eight of 15 VHH inhibited the cleavage of substrate SNAP25 (synaptosome-associated protein of 25,000 Da) by BoNT/A Lc. The most potent VHH (Aa1) had a solution K_d for BoNT/A Lc of 1.47 × 10^− 10 M and an IC₅₀ (50% inhibitory concentration) of 4.7 × 10^− 10 M and was resistant to heat denaturation and reducing conditions. To understand the mechanism by which Aa1 inhibited catalysis, we solved the X-ray crystal structure of the BoNT/A Lc-Aa1 VHH complex at 2.6 Å resolution. The structure reveals that the Aa1 VHH binds in the α-exosite of the BoNT/A Lc, far from the active site for catalysis. The study validates the utility of non-immune llama VHH libraries as a source of enzyme inhibitors and identifies the BoNT/A Lc α-exosite as a target for inhibitor development.     

利用137Cs技术研究黑土坡耕地土壤再分布特征

利用¹³⁷Cs示踪技术和不同的理论模型研究典型的东北漫岗地形的黑土土壤再分布状况.通过野外采样和模型分析,得出研究区¹³⁷Cs背景值为2 232.75 Bq·m^-2,介于长江三角洲和黄土高原背景值之间,表明¹³⁷Cs沉降与纬度和降水相关.研究区各地貌部位¹³⁷Cs含量在水平方向和深度分布上有很大的分异.坡肩部位¹³⁷Cs含量最低,土壤侵蚀最为强烈;坡顶和坡背侵蚀较为微弱;坡脚和坡足基本上表现土壤沉积.¹³⁷Cs分布深度从坡肩20 cm到坡足80 cm土层,表现出该区经历了强烈的侵蚀和沉积过程.文中采用4种常用的¹³⁷Cs土壤侵蚀模型估计研究区的土壤侵蚀速率,结果表明,PM模型明显低估了土壤侵蚀速率,MBM-1明显高估了土壤侵蚀速率,MBM-2和MBM-3估计的结果较为相近的合理结果.     

^125I-PLGA标记物的制备与鉴定

目的开发一种^125I-PLGA标记物合成的新技术,并对新合成标记物的性质进行鉴定。方法在密闭容器中,应用重复加热的方法,使Na^125I与PLGA氯仿溶液反应,实施PLGA的^125I放射性标记。并对^125I-PLGA标记物的放射性活度进行检测。结果经过上述标记过程,成功合成了^125I-PLGA标记物,此标记物释放γ射线,放射性活度和材料质量成正比。结论^125I-PLGA标记物与传统的放射性核素标记物相比,具有合成工艺简单、放射性污染易于控制、放射性活度易于检测等优点。