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91.
92.
M. H. D. LARMUSEAU B. HELLEMANS J. K. J. VAN HOUDT F. A. M. VOLCKAERT 《Molecular ecology resources》2007,7(1):147-149
A microsatellite‐enriched genomic library was constructed for the sand goby, Pomatoschistus minutus (Pallas 1770), and nine polymorphic DNA microsatellite markers of high quality were successfully optimized. Characterization of 96 individuals from the Vaccarès lagoon (France) showed moderate to high levels of polymorphism (two to 54 alleles). All the markers conformed to Hardy–Weinberg equilibrium and showed no evidence of null alleles, large allele dropout, stuttering and linkage disequilibrium between pairs of loci. These markers successfully amplify in three closely related species and can be employed to investigate population genetic structure and to clarify paternity in Pomatoschistus species. 相似文献
93.
METTE SERINE WESMAJERVI TEKLE TAFESE J
RGEN STENVIK KJERSTI TURID FJALESTAD B
RGE DAMSGRD MADJID DELGHANDI 《Molecular ecology resources》2007,7(1):138-140
One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di‐, tri‐, tetra‐ and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo‐G13 and Gmo‐G14 had a significant excess of homozygotes. All loci conformed to the Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci. 相似文献
94.
N. B. RODRIGUES M. R. SILVA M. M. PUCCI D. J. MINCHELLA R. SORENSEN P. T. LOVERDE A. J. ROMANHA G. OLIVEIRA 《Molecular ecology resources》2007,7(2):263-265
Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite‐enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively. 相似文献
95.
The selection of antibody fragments from libraries using in vitro screening technologies has proven to be a very good alternative to the classical hybridoma technology, and has overcome the laborious process of antibody humanization. However, the complexity of the library is critical in the probability of being able to directly isolate a high affinity antibody specific to a target. We report a method to make hyperdiversified antibody fragment libraries, based on human immunoglobulin variable genes mimicking the somatic hypermutation process. This mutagenesis technology, MutaGen, was used for the first time on the entire variable domain (frameworks and CDRs) of large repertoires of human variable antibody domains. Our MutaGen process uses low-fidelity human polymerases, known as mutases, suggested to be involved in the somatic hypermutation process of immunoglobulin genes. Depending on the mutases used, we generated complementary mutation patterns with randomly distributed mutations. The libraries were generated with an average of 1.8 mutations per 100 amino acids. The hyperdiversified antibody fragment libraries constructed with our process should enable the selection of antibody fragments specific to virtually any target. 相似文献
96.
Characteristics of the microbial community in rhizosphere of Camptotheca acuminata cultured with exotic invasive plant Eupatorium adenophorum 总被引:1,自引:0,他引:1
ZU YuanGang GAO ChongYang WANG WenJie YANG FengJian LIU Ying WANG Min ZHAO YangGuo 《中国科学:生命科学英文版》2007,50(1):22-30
The traditional culture-dependent plate counting and culture-independent small-subunit-ribosomal RNA gene-targeted molecular techniques, Single-Strand Conformation Polymorphism (SSCP) and ter-minal Restriction Fragment Length Polymorphism (tRFLP) combined with 16S rDNA clone library were adopted to investigate the impacts of secretion from Camptotheca acuminata (abbreviated to Ca) roots on the quantities and structure of eukaryotic microbes and bacteria in the rhizosphere, and the possi-bility that Ca controls exotic invasive plant Eupatorium adenophorum (Ea). The counting results indi-cated that the number of bacteria increased in turn in rhizospheres of Ea, Ca-Ea mixed culture and Ca, while that of eukaryotic microbes decreased. PCR-SSCP profiles showed eukaryotic microbial bands (corresponding to biodiversity) in rhizosphere of Ea were more complex than those of Ca and CE. Meristolohmannia sp., Termitomyces sp. and Rhodophyllus sp. were the dominant populations in the rhizosphere of Ca. Bacterial terminal restriction fragments (TRFs) profiles showed no difference among three kinds of rhizospheres, and the sequences of the 16S rDNA clone library from Ca rhizospheres were distributed in 10 known phyla, in which phylum Proteobacteria were the absolute dominant group and accounted for 24.71% of the cloned sequences (δ-Proteobacteria accounted for up to 17.65%), and phyla Acidobacteria and Bacteroidetes accounted for 16.47% and 10.59% of the cloned sequences, respectively. In addition, high performance liquid chromatography detected a trace amount of camp-tothecin and hydroxycamptothecin in the rhizospheric soil of Ca and CE, but examined neither camp-tothecin nor hydroxycamptothecin in rhizospheric soil of Ea. Therefore, invasion and diffusion of Ea evidently depended on distinguishing the eukaryotic community structure, but not on that of the bac-terial pattern. Ca was able to alter the eukaryotic community structure of invasive Ea by secreting camptothecin and hydroxycamptothecin into rhizospheres, and may benefit the control of overspread of Ea. This study provided theoretical evidence for rhizospheric microbial aspects on substituting Ca for Ea. 相似文献
97.
98.
Michael Adsetts Edberg Hansen Jørn Smedsgaard 《Metabolomics : Official journal of the Metabolomic Society》2007,3(1):41-54
The use of mass spectrometry (MS) is pivotal in analyses of the metabolome and presents a major challenge for subsequent data
processing. While the last few years have given new high performance instruments, there has not been a comparable development
in data processing. In this paper we discuss an automated data processing pipeline to compare large numbers of fingerprint
spectra from direct infusion experiments analyzed by high resolution MS. We describe some of the intriguing problems that
have to be addressed, starting with the conversion and pre-processing of the raw data to the final data analysis. Illustrated
on the direct infusion analysis (ESI-TOF-MS) of complex mixtures the method exploits the full quality of the high-resolution
present in the mass spectra. Although the method is illustrated as a new library search method for high resolution MS, we
demonstrate that the output of the preprocessing is applicable to cluster-, discriminant analysis, and related multivariate
methods applied directly to mass spectra from direct infusion analysis of crude extracts. This is done to find the relationship
between several terverticillate Penicillium species and identify the ions responsible for the segregation. 相似文献
99.
Davide Perini Marilena Meloni Kirsten Wolff Giorgio Binelli 《Conservation Genetics》2007,8(5):1263-1266
The hexaploid herbaceous species Primula glaucescens Moretti and Primula spectabilis Tratt. (2n = 6x = 66) are two endemics of the Italian Pre-Alps protected by national and international laws. In order to plan conservation
strategies for natural populations and to study the influence of the latest glaciation on them we developed a set of microsatellites
markers for the endangered Primula species: ten primer pairs allowed analysis of polymorphic disomic loci in P. glaucescens samples and seven of them also amplified polymorphic disomic markers in P. spectabilis.
Kirsten Wolff and Giorgio Binelli contributed equally to the paper 相似文献
100.
图书馆开展大学生信息素养教育的优势与途径 总被引:3,自引:0,他引:3
论述了对大学生进行信息素养教育的意义,阐明了图书馆在开展大学生信息素养教育过程中起到的优势作用,提出了利用图书馆进行大学生信息素养教育的6种途径。 相似文献