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1.
Madjid Delghandi Mette Serine Wesmajervi Saskia Mennen Frank Nilsen 《Conservation Genetics》2008,9(5):1395-1398
Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide
motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to
10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed
significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These
microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage
maps of Atlantic cod. 相似文献
2.
Madjid Delghandi Mette Serine Wesmajervi Saskia Mennen Frank Nilsen 《Conservation Genetics》2009,10(4):1037-1040
Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of
Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96
unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed
and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed
significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers.
These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing
linkage maps of Atlantic cod. 相似文献
3.
Jon-Ivar Westgaard Tekle Tafese Mette Serine Wesmajervi Frank Nilsen Kjersti Turid Fjalestad Børge Damsgård Madjid Delghandi 《Conservation Genetics》2007,8(6):1503-1506
Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic
cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs
and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus
and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of
the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers.
The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
Jon-Ivar Westgaard and Tekle Tafese have contributed equally to the work. 相似文献
4.
Jon-Ivar Westgaard Tekle Tafese Mette Serine Wesmajervi Jørgen Stenvik Kjersti Turid Fjalestad Børge Damsgård Madjid Delghandi 《Conservation Genetics》2007,8(3):749-751
A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population.
The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122–0.907 and
0.673–0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy–Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination
of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing
linkage maps of Atlantic cod.
Jon-Ivar Westgaard and Tekle Tafese have contributed equally to the work. 相似文献
5.
C. E. EDWARDS M. ARAKAKI P. F. QUINTANA‐ASCENCIO D. E. SOLTIS P. S. SOLTIS 《Molecular ecology resources》2007,7(6):1135-1137
We report the isolation of 19 primer pairs for amplification of polymorphic microsatellite loci for Hypericum cumulicola. These markers were evaluated in 24 individuals from one population; two to four alleles were detected per locus, and observed heterozygosity ranged from 0 to 0.5. Two loci demonstrated significant heterozygote deficiencies, possibly due to null alleles, and significant linkage disequilibrium was found between six pairs of loci. The remaining microsatellite loci will help determine if genetic differentiation is responsible for life‐history differences between natural and anthropogenically disturbed populations of H. cumulicola. 相似文献
6.
A microsatellite‐enriched genomic library was obtained for the threatened brook lamprey, Lethenteron sp. N, and from it 16 dinucleotide markers were successfully isolated and characterized. These markers were found to have between 1 and 6 alleles with heterozygosity ranging from zero to 0.79. Cross‐species amplification was successful, with eight loci amplifying products in the counterpart cryptic species, Lethenteron sp. S, as well as 15 loci in Lethenteron japonicum. One out of 10 primer pairs previously reported also amplified products in all the three species. 相似文献
7.
We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty‐two primer pairs cross‐amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross‐amplified in longfin smelt (Spirinchus thaleichthys). 相似文献
8.
Ten primer pairs were designed from two genomic libraries enriched for (GACA)4 and (GACA)7 in the sailfin silverside Telmatherina antoniae. Characterization with 57 T. antoniae individuals revealed between three and 30 alleles, with observed and expected heterozygosity values ranging from 0.47 to 0.98 and from 0.46 to 0.93, respectively. Eight of the 10 loci conformed to Hardy–Weinberg equilibrium, with no significant linkage detected between loci pairs. These microsatellite markers are intended for use in population genetic studies of T. antoniae and related fishes of the family Telmatherinidae. 相似文献
9.
Jing-Feng Yang Li-Li Zhao Chang-Wei Shao Xiang-Shan Ji Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(5):1541-1543
Black snapper Sebastodes fuscescens (Houttuyn) is an economocally important species. Fourty-five microsatellite loci were isolated from an enriched genomic library
of S. fuscescens. Ten of these loci were polymorphic in a test population with alleles per locus ranging from 2 to 5, and observed and expected
heterozygosities per locus from 0.21 to 1.00 and from 0.19 to 0.86, respectively. Three loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic
microsatellite loci would be useful for investigating genetic diversity of S. fuscescens and other related species. 相似文献
10.
Isolation and characterization of polymorphic microsatellite loci from fat greenling (Hexagrammos otakii) 总被引:2,自引:0,他引:2
Song-Lin Chen Gui-Dong Miao Chang-Wei Shao Yong-Sheng Tian Xiaolin Liao 《Conservation Genetics》2009,10(5):1429-1431
Fat greenling (Hexagrammos otakii) is an economocally important marine fish species. Thirtyfive microsatellite loci were isolated from two enriched genomic
library of Hexagrammos otakii. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.2581 to 1.0000 and from 0.2892 to 0.7726, respectively. Four loci significantly
deviated from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between
pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Hexagrammos otakii. 相似文献
11.
Song-Lin Chen Shi-Chao Xing Gen-Bo Xu Xiaolin Liao Jing-Feng Yang 《Conservation Genetics》2009,10(5):1469-1471
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library
of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis. 相似文献
12.
We report the isolation and characterization of polymorphic microsatellite loci for the Siberian flying squirrel Pteromys volans. The seven most useful loci had between six and 11 alleles and expected heterozygosities ranging from 0.477 to 0.866. We also tested the utility of these loci in other squirrel species, northern flying squirrels (Glaucomys sabrinus and G. volans) and the common red squirrel (Sciurus vulgaris). Three of the Siberian flying squirrel loci were polymorphic in other squirrel species, suggesting a limited potential for cross‐species use. 相似文献
13.
Yanhong Sun Dongmei Yu Guangjian Zhu Xusheng Liu Shuyi Zhang Jinping Chen 《Conservation Genetics》2009,10(6):1857-1859
We used enriched genomic library method to isolate and characterize 11 di-nucleotide microsatellite loci in the Lesser Asiatic
Yellow House Bat, Scotophilus kuhlii. The polymorphism of these loci was tested on a natural population of 36 individuals from Hainan Province, South China. All
loci revealed the polymorphism ranging from 5 to 18 alleles. The observed heterozygosity values were from 0.694 to 1.000 and
expected heterozygosity values were from 0.558 to 0.929. No significant linkage disequilibrium was detected between any pairs
of loci. These polymorphic markers will be used to assess population structure in S. kuhlii. 相似文献
14.
Isolation and characterization of Ligustrum micranthum (Oleaceae) microsatellite loci using paired‐end Illumina reads 下载免费PDF全文
Twenty‐six microsatellite loci were developed and characterized for Ligustrum micranthum, a species endemic to the Ogasawara Islands, Japan. The genetic structure of this species must be clarified in order to restore the island's ecosystem. A total of 8511 primer pairs were designed from de novo sequencing. Of the 48 primer pairs selected, amplification and polymorphisms were tested using one population each from the Chichijima and Hahajima Islands of the Ogasawara Islands. Twenty‐six microsatellite loci were successfully amplified and the number of alleles for these loci ranged from five to 31 per locus, and the mean expected heterozygosities were 0.858 and 0.849, respectively. No significant deviation from the Hardy–Weinberg equilibrium was observed in either population, and no significant linkage disequilibrium was detected between any locus pair. The microsatellite loci reported in this study can be used in future studies to evaluate the genetic structure and mating system of L. micranthum. 相似文献
15.
G. Pellegrino D. Cafasso A. Widmer M. Soliva A. Musacchio S. Cozzolino 《Molecular ecology resources》2001,1(4):279-280
In this paper we describe the isolation and characterization of six polymorphic microsatellite loci from the orchid Serapias vomeracea. This species is widely distributed in the Mediterranean region. Microsatellite loci were isolated from an enriched library and primer pairs were designed for 18 loci. Primer pairs for six loci amplified well and were tested on samples from southern Italy. Levels of genetic variability detected at these six loci are high, with numbers of alleles per locus ranging from 3 to 6, and observed heterozygosity (HO) ranging from 0.35 to 0.86. All primer pairs tested amplified DNA from four other Serapias species, indicating that the primers are useful for population genetic studies throughout the genus. 相似文献
16.
J. A. GALARZA G. F. TURNER E. MACPHERSON J. CARRERAS‐CARBONELL C. RICO 《Molecular ecology resources》2007,7(2):230-232
Ten polymorphic dinucleotide microsatellite loci were isolated and characterized for the red mullet (Mullus barbatus). Allele variability was tested on both the red mullet and its congener the striped red mullet (Mullus surmuletus). Characterization of 30 individuals of both species from the western Mediterranean showed moderate to high allelic diversity ranging from two to 26 alleles per locus (mean 10.9). Three loci showed departures from Hardy–Weinberg proportions. No evidence of significant association between genotypes at pairs of loci was observed. These polymorphic loci could be suitable for population genetic assessments of both species. 相似文献
17.
Ying Y. Zhao Jiao Li Xiao W. Sun Li Q. Liang Xiao D. Zhu Hong S. Ou-yang 《Conservation Genetics》2009,10(4):1005-1007
Ten polymorphic microsatellite markers were developed to examine the population structure of the burbot (Lota Lota). The number of alleles ranging from 2 to 14 and the expected heterozygosity ranging from 0.306 to 0.926 were screened in
the wild samples from Wusuli River in Heilongjiang Province, China. Eight loci significantly deviated from Hardy–Weinberg
equilibrium. No evidence showed significant linkage disequilibrium between pairs of loci. These newly isolated markers would
also enrich the available molecular resources of the burbot. 相似文献
18.
Weiwei Dong Huiwu Tian Dengqiang Wang Lixiong Yu Xinbin Duan Shaoping Liu Daqing Chen 《Zeitschrift fur angewandte Ichthyologie》2019,35(6):1295-1299
Gobiobotia filifer is a small benthic fish distributed in Yangtze River Basin. The abundance of G. filifer increased after impoundment of Xiluodu Dam and Xiangjiaba Dam. The state of population structure and changes of genetic diversity before and after impoundment of Xiluodu Dam and Xiangjiaba Dam were interesting issues. However, efficient molecular markers were rare, which will limit us to solve above problems. Twenty‐eight expressed sequence tag SSRs (EST‐SSRs) were successfully identified and verified as stable amplification and polymorphic loci by polyacrylamide gel electrophoresis (PAGE) and capillary electrophoresis. The number of alleles at these EST‐SSR loci ranged from 3 to 14, the polymorphism information content values were 0.125–0.897, and the observed and expected heterozygosities were 0.0–0.857 and 0.132–0.928, respectively. Cross‐species amplification of the 28 loci developed in this study was examined in seven individuals of each of the 7 taxa. The amplification efficiency of 28 EST‐SSRs primer pairs is related to the distance of genetic relationship between cross‐species with G. filifer, and same subfamily species (Xenophysogobio boulengeri and Xenophysogobio nudicorpa) showed the highest (50%) amplification efficiency. These EST‐SSR markers could be used to analyse genetic diversity and population structure of G. filifer and related species. 相似文献
19.
Seven-band grouper (Epinephelus septemfasciatus) is a commercially important fishery species. Sixty-six microsatellite loci were isolated from a dinucleotide-enriched genomic
library of E. septemfasciatus. Twelve of these loci were polymorphic in a test population with alleles per locus ranging from two to five, and observed
and expected heterozygosities per locus from 0.04 to 1.00 and from 0.28 to 0.76, respectively. Three loci significantly deviated
from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci after Bonferroni
correction. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of E. septemfasciatus and other related species.
Lili Zhao and Changwei Shao have contributed equally. 相似文献
20.
Song-Lin Chen Lili Zhao Chang-Wei Shao Xiaolin Liao Yong-Sheng Tian 《Conservation Genetics》2009,10(4):1101-1103
Black sea bass (Centropristis striata) is an economically important serranid species. A number of 39 microsatellite loci were isolated from two enriched genomic
library of C. striata. Eleven of these loci were polymorphic in a test population with alleles per locus ranging from 3 to 8, and observed and
expected heterozygosities per locus from 0.26 to 1.00 and from 0.61 to 0.84, respectively. Four loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of C. striata and other related species. 相似文献