全文获取类型
收费全文 | 722篇 |
免费 | 60篇 |
国内免费 | 214篇 |
出版年
2023年 | 14篇 |
2022年 | 29篇 |
2021年 | 28篇 |
2020年 | 26篇 |
2019年 | 42篇 |
2018年 | 28篇 |
2017年 | 31篇 |
2016年 | 26篇 |
2015年 | 36篇 |
2014年 | 55篇 |
2013年 | 72篇 |
2012年 | 37篇 |
2011年 | 55篇 |
2010年 | 37篇 |
2009年 | 34篇 |
2008年 | 39篇 |
2007年 | 43篇 |
2006年 | 34篇 |
2005年 | 44篇 |
2004年 | 34篇 |
2003年 | 35篇 |
2002年 | 32篇 |
2001年 | 24篇 |
2000年 | 24篇 |
1999年 | 14篇 |
1998年 | 14篇 |
1997年 | 14篇 |
1996年 | 6篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1958年 | 1篇 |
1950年 | 1篇 |
排序方式: 共有996条查询结果,搜索用时 15 毫秒
91.
92.
胜利河连续系统中蜉蝣优势种的生产量动态和营养基础 总被引:1,自引:0,他引:1
大型底栖动物在河流生态系统中发挥着重要作用, 2009 年3月至2010 年3月间对长江中游支流巴河流域的胜利河大型底栖动物群落优势种类的生产力进行为期1 周年的调查研究, 结果表明, 主要蜉蝣优势种扁蜉、等蜉和红斑蜉的生活史为3代/年、3代/年和 2代/年。现存量呈现出1-3级河流增加,而4级又较3级有所下降的趋势。采用龄期频率法( instar-frequency method) 测算的平均周年生产量分别为扁蜉, 200.13 g/(m2.a) , P/B 为23.69; 等蜉, 82.06 g/(m2.a), P/B 为18.12;红斑蜉, 12.30 g/(m2.a), P/B 为8.78。三种蜉蝣的生产量动态在时间上与现存量动态较一致,但彼此各不相同。扁蜉的日均产量于2009年3月在二级河流中达到最大(363.56 mg/m2.d),等蜉于2010年3月在三级河流中到达最大(282.76 mg/m2.d),而红斑蜉于2009年3月在一级河流中到达最大(33.36 mg/m2.d)。生产量的营养基础分析结果表明,扁蜉前肠内含物中无形态碎屑、动物组织、植物纤维、丝状藻类、硅藻所占平均比例为74.37%,4.19%,17.11%,4.29%,0.04%,对生产量的贡献率分别为77.15%,11.27%,6.57%,4.95% ,0.04%;等蜉前肠内含物中无形态碎屑、动物组织、植物纤维、真菌、丝状藻类和硅藻所占平均比例为65.64%,6.17%,23.04%,0.54%,4.53%,对生产量的贡献率分别为: 68.16%,16.61%,8.86%,1.03%,5.23%,0.09%,0.10%;红斑蜉前肠内含物中,无形态碎屑、动物组织、植物纤维、真菌、丝状藻类和硅藻所占平均比例为41.14%,5.96%,38.04%,1.34%,11.21%,2.31%,对生产量的贡献率分别为46.67%,17.52%,15.98%,2.81%,14.13%,2.91%。这与我们在黑竹冲和叹气沟的研究结果存在一定差异,可能与这些溪流自身环境和地区分布有关。 相似文献
93.
黔西北铅锌矿区植物群落分布及其对重金属的迁移特征 总被引:9,自引:0,他引:9
重金属耐性植物和超富集植物的筛选、鉴定和驯化是植物修复技术研究与发展的关键。以黔西北4个不同恢复年限的铅锌矿为研究对象,通过群落生态调查利用聚类分析方法筛选出研究区域中重金属耐性植物优势种,并分析其对重金属Pb、Zn、Cu、Cd的迁移富集能力。结果表明:4个矿区共发现高等植物22种,分属13科21属,筛选出9种重金属耐性植物优势种,其中转运系数大于1的植物有:黄花蒿(Cu)、珠光香青(Zn)、大叶醉鱼草(Zn/Pb/Cd)、野艾蒿(Cu/Zn/Pb/Cd);没有富集系数大于1的植物。其中大叶醉鱼草具有耐贫瘠、耐旱、生物量大等优势,可将其作为典型的重金属耐性先锋植物,用于矿区废弃地的植物修复。 相似文献
94.
95.
Relationships among species assigned to the yeast genera Pichia, Issatchenkia and Williopsis , which are characterized by the ubiquinone CoQ-7 and inability to utilize methanol, were phylogenetically analyzed from nucleotide sequence divergence in the genes coding for large and small subunit rRNAs and for translation elongation factor-1α. From this analysis, the species separated into five clades. Species of Issatchenkia are members of the Pichia membranifaciens clade and are proposed for transfer to Pichia . Pichia dryadoides and Pichia quercuum are basal members of the genus Starmera . Williopsis species are dispersed among hat-spored taxa in each of the remaining three clades, which are proposed as the new genera Barnettozyma, Lindnera and Wickerhamomyces . Lineages previously classified as varieties of Pichia kluyveri , ' Issatchenkia ' scutulata, Starmera amethionina and ' Williopsis ' saturnus are elevated to species rank based on sequence comparisons. 相似文献
96.
TRPP2 (transient receptor potential polycystin-2) channels function in a range of cells where they are localized to specific subcellular regions including the endoplasmic reticulum (ER) and primary cilium. In humans, TRPP2/PC-2 mutations severely compromise kidney function and cause autosomal dominant polycystic kidney disease (ADPKD). The Caenorhabditis elegans TRPP2 homolog, PKD-2, is restricted to the somatodendritic (cell body and dendrite) and ciliary compartments of male specific sensory neurons. Within these neurons PKD-2 function is required for sensation. To understand the mechanisms regulating TRPP2 subcellular distribution and activity, we performed in vivo structure-function-localization studies using C. elegans as a model system. Our data demonstrate that somatodendritic and ciliary targeting requires the transmembrane (TM) region of PKD-2 and that the PKD-2 cytosolic termini regulate subcellular distribution and function. Within neuronal cell bodies, PKD-2 colocalizes with the OSM-9 TRP vanilloid (TRPV) channel, suggesting that these TRPP and TRPV channels may function in a common process. When human TRPP2/PC-2 is heterologously expressed in transgenic C. elegans animals, PC-2 does not visibly localize to cilia but does partially rescue pkd-2 null mutant defects, suggesting that human PC-2 and PKD-2 are functional homologs. 相似文献
97.
Dysfunction of E-cadherins often results in metastasis of cancerous cells. Here we show that p35, a critical regulator of cyclin-dependent kinase 5 (CDK5), specifically depletes the precursor form of E-cadherin, but not the mature form, by using a precursor-specific antibody. Most intriguingly, this downregulation of precursor E-cadherin by p35 is unequivocally independent of CDK5. Moreover, we found that p35 forms complexes with E-cadherin proteins. We also found that p35 co-expression can target E-cadherin to lysosomes and that p35-triggered disappearance of E-cadherin precursor can be blocked specifically by lysosomal protease inhibitors, indicating that p35 induces endocytosis and subsequent degradation of precursor E-cadherin. 相似文献
98.
99.
100.
Humphrey GW Wang YH Hirai T Padmanabhan R Panchision DM Newell LF McKay RD Howard BH 《Differentiation; research in biological diversity》2008,76(4):348-356
Abstract In eukaryotic cells, covalent modifications to core histones contribute to the establishment and maintenance of cellular phenotype via regulation of gene expression. Histone acetyltransferases (HATs) cooperate with histone deacetylases (HDACs) to establish and maintain specific patterns of histone acetylation. HDAC inhibitors can cause pluripotent stem cells to cease proliferating and enter terminal differentiation pathways in culture. To better define the roles of individual HDACs in stem cell differentiation, we have constructed "dominant-negative" stem cell lines expressing mutant, Flag-tagged HDACs with reduced enzymatic activity. Replacement of a single residue (His→Ala) in the catalytic center reduced the activity of HDACs 1 and 2 by 80%, and abolished HDAC3 activity; the mutant HDACs were expressed at similar levels and in the same multiprotein complexes as wild-type HDACs. Hexamethylene bisacetamide-induced MEL cell differentiation was potentiated by the individual mutant HDACs, but only to 2%, versus 60% for an HDAC inhibitor, sodium butyrate, suggesting that inhibition of multiple HDACs is required for full potentiation. Cultured E14.5 cortical stem cells differentiate to neurons, astrocytes, and oligodendrocytes upon withdrawal of basic fibroblast growth factor. Transduction of stem cells with mutant HDACs 1, 2, or 3 shifted cell fate choice toward oligodendrocytes. Mutant HDAC2 also increased differentiation to astrocytes, while mutant HDAC1 reduced differentiation to neurons by 50%. These results indicate that HDAC activity inhibits differentiation to oligodendrocytes, and that HDAC2 activity specifically inhibits differentiation to astrocytes, while HDAC1 activity is required for differentiation to neurons. 相似文献