卡前列素氨丁三醇注射液联合缩宫素对剖宫产出血患者FIB与D-二聚体的影响

目的:探讨卡前列素氨丁三醇注射液联合缩宫素用于剖宫产产后出血的预防效果及对纤维蛋白原(FBI)、D-二聚体(D-D)水平的影响。方法:选择2014年1月至2016年10月我院接诊的92例剖宫产产妇,通过随机数表法分为观察组(n=46)和对照组(n=46)。对照组在胎儿娩出后给予缩宫素的宫内注射及静脉滴注,观察组在胎儿娩出后,先给予缩宫素的宫内注射,再在子宫肌壁注射卡前列素氨丁三醇注射液。比较两组产后出血情况、宫缩持续时间、子宫底下降速度、恶露持续时间、FIB、D-D水平及不良反应的发生情况。结果:观察组产后2 h、24 h出血量及产后出血率明显低于对照组(P0.05),宫缩持续时间长于对照组(P0.05),子宫底下降速度快于对照组(P0.05),恶露持续时间明显短于对照组组(P0.05)。两组治疗前FIB、D-D水平比较差异无统计学意义(P0.05)。治疗后,两组FIB、D-D水平均较治疗前显著降低(P0.05),且观察组FIB、D-D水平均明显低于对照组(P0.05);两组恶心呕吐、面部潮红、胸闷、头痛发生率比较差异无统计学意义(P0.05)。结论:在剖宫产产妇中应用卡前列素氨丁三醇注射液联合缩宫素可降低产后出血发生率,且安全性高,可能与有效减少FIB、D-D表达有关。     

负压封闭引流技术联合前列地尔注射液对重度烧伤患者的疗效观察

目的:研究负压封闭引流技术联合前列地尔注射液对重度烧伤患者的疗效观察。方法:选取2017年2月至2018年2月的67例患者。按照随机数表法分为观察组(n=36)和对照组(n=31),对照组采用负压封闭引流技术治疗,观察组采用负压封闭引流技术联合前列地尔注射液治疗。观察两组治疗疗效情况,临床治疗指标(创面愈合时间、创面愈合率、VAS评分、住院时间),C反应蛋白(CRP)、降钙素原(PCT)水平,不良反应发生率。结果:治疗后,观察组总有效率显著高于对照组[86.11%(31/36)vs 61.29%(19/31)](P0.05);创面愈合时间、创面愈合率、VAS评分、住院时间均显著低于对照组[(16.93±4.19)天vs(24.27±6.01)天,(92.72±5.03)%vs(81.34±2.48)%,(2.93±0.52)分vs(6.70±1.30)分,(24.09±7.43)天vs(46.98±10.72)天](P0.05);CRP、PCT水平均显著低于对照组[(27.93±3.81)mg/L vs(83.86±7.43)mg/L,(0.10±0.02)ng/L vs(0.43±0.06)ng/L](P0.05);不良反应总发生率显著低于对照组[8.33%(3/36)vs 38.70%(12/31)](P0.05)。结论:负压封闭引流技术联合前列地尔注射液对重度烧伤患者的疗效显著,可促进创面恢复,避免感染,并发症少,安全可靠。     

The contrary functions of lncRNA HOTAIR/miR‐17‐5p/PTEN axis and Shenqifuzheng injection on chemosensitivity of gastric cancer cells

This study was implemented to figure out whether lncRNA HOTAIR/miR‐17‐5p/PTEN axis played a role that was opposite to Shenqifuzheng (SQFZ) injection in regulating the chemosensitivity of gastric cancer cells. The gastric cancer tissues were gathered and four gastric cancer cell lines were prepared, including BGC‐823, MGC‐803, SGC‐7901, and MKN28. Moreover, cisplatin, adriamycin, mitomycin, and 5‐fluoroura were managed as the chemo‐therapeutics, and SQFZ was prepared as a Chinese medicine. Striking distinctions of HOTAIR, miR‐17‐5p, and PTEN expressions were observed between gastric cancer tissues and para‐carcinoma normal tissues (P < 0.05). MKN28 was associated with the highest resistance to cisplatin, adriamycin, mitomycin, and 5‐fluoroura among all the cell types, and SQFZ significantly improved the MKN28 cells’ sensitivity to the drugs (P < 0.05). The over‐expressed HOTAIR and miR‐17‐5p, as well as under‐expressed PTEN tended to significantly facilitate the viability, EMT process and proliferation of MKN28 cells that were subject to treatment of chemo‐therapies (P < 0.05). SQFZ could amplify the effects of si‐HOTAIR, miR‐17‐5p inhibitor, and pcDNA‐PTEN on boosting the chemosensitivity of gastric cancer cells (P < 0.05). In addition, HOTAIR was also found to directly target miR‐17‐5p, and PTEN appeared to be subject to the modification of HOTAIR and miR‐17‐5p in its acting on the viability, proliferation, EMT process, and apoptosis of gastric cancer cells. The HOTAIR/miR‐17‐5p/PTEN axis could be regarded as the potential treatment targets for gastric cancer, and adjuvant therapy of SQFZ injection could assist in further improving the treatment efficacy of chemo‐therapies for gastric cancer.     

Assessment of cerebral hemodynamics during neurosurgical procedures using laser speckle image analysis

Aneurysmal subarachnoid hemorrhage (aSAH) is a severe medical condition associated with a significant cause of mortality throughout the world. Cisterna magna injection model is accepted widely to mimic clinical aSAH and is performed on small animal models to study aSAH during neurosurgery. Coherent light scattered from the surface of the rat brain is used to infer information about the variations in blood flow during this condition. We obtained speckle images from the exposed cortex during the entire experiment using an external tissue imaging system. Contrast and fractal analyses are carried out for the recorded speckle pattern time series. Correlation analysis based on Hurst exponent for these images is found to be a more sensitive tool in studying aSAH as compared to routinely used laser speckle contrast analysis for assessing the changes in blood flow velocity. Additionally, our studies provide improved blood flow detection sensitivity with image Hurst exponent in combination with computed fractal dimension, during an event of aSAH.     

Efficient generation of transgenic mice by direct intraovarian injection of plasmid DNA

We established a rapid procedure for obtaining transgenic mice by directly injecting an enhanced green fluorescent protein (EGFP)-expressing plasmid (pIRES-EGFP) into the ovaries of fertile mice. The frequency of transgenic mouse production was determined by pair-mating, and by polymerase chain reaction (PCR) and sequence analysis of DNA taken from the tails of the offspring. The mice that received the EGFP gene transmitted it to their offspring (F(1)). Genetic and PCR analyses of F(1) progeny confirmed that the inserted EGFP was stably inherited. Of six female F(1) mice, all were able to pass the foreign DNA on to the next generation (F(2)). In situ hybridization using paraffin-embedded sections of ovarian and testicular tissues from the F(1) and F(2) progeny showed that the introduced gene was expressed in the gonads of the animals. The chromosomal location of the injected DNA was determined by fluorescence in situ hybridization, and the frequency of multiple site versus single site insertions is 85.71% (18/21) analyzed by FISH. We anticipate great progress in murine genetic engineering using this technique.     

Temperate phages TP901-1 and phiLC3, belonging to the P335 species, apparently use different pathways for DNA injection in Lactococcus lactis subsp. cremoris 3107

Five mutants of Lactococcus lactis subsp. cremoris 3107 resistant to phage TP901-1 were obtained after treatment with ethyl methanesulfonate. Two of the mutants were also resistant to phage phiLC3. The remaining three mutants were as sensitive as 3107. Mutants E46 and E100 did not adsorb the two phages. Mutants E119, E121 and E126 adsorbed phage phiLC3 as well as 3107 but phage TP901-1 with significantly reduced efficiency. All, except E46, could be lysogenized with phage TP901-BC1034, a derivative of TP901-1 harboring an erythromycin-resistance marker. However, the lysogenization frequency was 10(3)-10(4) fold higher for 3107 than for the mutants. Mitomycin C induction of lysogenized mutants 3107 indicated that phage propagation was not affected in these four mutants. Electron microscopy and analysis of total DNA of infected cells showed that DNA was liberated from the phage particle during infection of strain 3107 with TP901-1 and that intracellular phage DNA replication occurred. This was not the case for mutants E121 and E126. This strongly suggests that some step starting with triggering DNA release and ending with DNA injection is impaired during infection with TP901-1. As such impairment was not seen when infecting E119, E121 and E126 with phiLC3, we conclude that TP901-1 and phiLC3 either are differently triggered by their receptor or utilize different pathways of injection.     

杏芎氯化钠注射液联合依达拉奉对急性脑梗死患者神经功能、脑血流灌注及氧化应激水平的影响

摘要 目的：探讨杏芎氯化钠注射液联合依达拉奉对急性脑梗死（ACI）患者神经功能、脑血流灌注及氧化应激水平的影响。方法：随机将2018年1月至2019年7月我院收治的120例ACI患者分为两组,均给予常规神经内科治疗,对照组（60例）在常规治疗基础上给予依达拉奉静脉滴注2周,观察组（60例）在对照组基础上加用杏芎氯化钠注射液滴注两周。观察两组临床疗效和安全性,并比较治疗前后神经功能、认知功能、残疾程度、脑血流灌注以及氧化应激水平的差异。结果：观察组治疗总有效率均高于对照组（P＜0.05）。观察组治疗后美国国立研究院脑卒中评定量表（NIHSS）、改良RANKIN量表（MRS）评分、脑缺血区平均通过时间(MTT)、峰值时间 (TTP)、丙二醛(MDA)、晚期氧化蛋白产物(AOPP)低于对照组（P＜0.05）,简易智能精神状态检查量表(MMSE)评分、脑血流量(CBF)、脑血容量(CBV)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC )高于对照组（P＜0.05）。两组不良反应率比较无统计学差异（P＞0.05）。结论：杏芎氯化钠注射液联合依达拉奉治疗可有效地改善ACI患者神经功能,增加缺血区脑血流灌注量,改善氧化应激,提高临床疗效。     

参芪扶正注射液联合多西他赛对乳腺癌患者外周血象、免疫功能及血清肿瘤标志物的影响

摘要 目的：观察参芪扶正注射液联合多西他赛对乳腺癌患者外周血象、免疫功能及血清肿瘤标志物的影响。方法：选取2018年1月~2019年12月我院收治的64例乳腺癌患者,根据信封抽签法将患者分为对照组（n=32,多西他赛治疗）和研究组（n=32,参芪扶正注射液联合多西他赛治疗）。对比两组疗效,对比两组治疗前、治疗2个疗程后的外周血象、免疫功能及血清肿瘤标志物,记录两组治疗期间不良反应情况。结果：比较两组不良反应无差异（P>0.05）。治疗2个疗程后,对照组的总有效率为43.75%（14/32）,研究组的总有效率为68.75%（22/32）,研究组的总有效率高于对照组（P<0.05）。治疗2个疗程后,两组血红蛋白、血小板、白细胞水平均较治疗前下降,但研究组高于对照组（P<0.05）。两组CD3⁺、CD4⁺/CD8⁺、CD80、CD86水平均较治疗前下降,但研究组高于对照组（P<0.05）。治疗2个疗程后,两组癌胚抗原（CEA）、糖蛋白 125（CA125）、糖蛋白 153（CA153）水平均较治疗前下降,且研究组低于对照组（P<0.05）。结论：参芪扶正注射液联合多西他赛治疗乳腺癌患者,可有效减少肿瘤标志物分泌,抑制肿瘤生长,减轻机体免疫抑制,改善外周血象,且安全可靠。     

双歧杆菌三联活菌胶囊与血必净注射液联合治疗术后早期炎性肠梗阻的疗效及对肠黏膜功能的影响

目的:研究双歧杆菌三联活菌胶囊与血必净注射液联合治疗术后早期炎性肠梗阻(EPISBO)的疗效及对肠黏膜功能和肠壁黏膜炎症的影响。方法:选择从2018年10月到2020年8月在医院接受治疗的EPISBO患者100例作为观察对象,按随机数表法将其分成观察组及对照组各50例,两组均常规治疗,对照组另给予血必净注射液,观察组在对照组的用药基础上再增用双歧杆菌三联活菌胶囊。两组均治疗7d,对比两组疗效,胃肠功能相关指标,肠黏膜功能相关指标,肠壁黏膜炎症指标,以及不良反应。结果:观察组的总有效率为98.00%,明显高于对照组的84.00%(均P<0.05)。观察组的胃肠功能相关指标均分别明显优于对照组(均P<0.05)。治疗7d后,两组的丙二醛(MDA)、二胺氧化酶(DAO)及乳酸(D-Lac)水平均明显低于治疗前,且观察组又低于对照组(P<0.05)。两组的超氧化物歧化酶(SOD)水平明显高于治疗前,且观察组又高于对照组(P<0.05)。治疗7d后两组的内毒素(LPS)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及IL-1β水平均明显低于治疗前,且观察组又低于对照组(P<0.05)。两组的不良反应相比,差异不显著(P>0.05)。结论:应用双歧杆菌三联活菌胶囊联合血必净注射液治疗EPISBO的疗效和安全性均较好,且对肠黏膜功能和肠壁黏膜炎症具有较好的改善作用,值得推广。     

舒血宁注射液联合丹参滴丸对急性冠脉综合征患者血脂和心功能的影响

摘要 目的：探讨舒血宁注射液联合丹参滴丸对急性冠脉综合征（ACS）患者血脂和心功能的影响。方法：选取2015年2月~2019年12月期间我院收治的ACS患者90例,按照随机数字表法将上述患者分为对照组（n=45,常规基础治疗）和研究组（n=45,增加舒血宁注射液联合丹参滴丸治疗）,比较两组患者疗效、血脂、心功能及不良反应。结果：研究组治疗2个疗程后的临床总有效率为91.11%（41/45）,高于对照组的73.33%（33/45）（P<0.05）。两组治疗2个疗程后总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）均较治疗前降低,且研究组低于对照组（P<0.05）;高密度脂蛋白胆固醇（HDL-C）较治疗前升高,且研究组高于对照组（P<0.05）。两组治疗2个疗程后左心室射血分数（LVEF）、心脏指数（CI）、心排血量（CO）均较治疗前升高,且研究组高于对照组（P<0.05）;左室舒张末期内径（LVEDD）较治疗前降低,且研究组低于对照组（P<0.05）。两组治疗期间不良反应发生率比较差异无统计学意义（P>0.05）。结论：舒血宁注射液联合丹参滴丸治疗ACS,疗效显著,可有效改善患者血脂及心功能,且不增加不良反应发生率,安全可靠。